##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BcgI BclI BfrI
BglII BlnI BsaAI BsaHI BsaI
BsiWI BsmBI BspDI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraIII Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MflI
MluI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 76 enzymes) #
###################################
AccII 61 CGCG CG^CG
AccIII 52 TCCGGA T^CCGGA
AflIII 1102 ACGTGT A^C[GA][CT]GT
AseI 351 ATTAAT AT^TAAT
AsnI 351 ATTAAT AT^TAAT
AspI 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 144 CCCGGG C^[CT]CG[GA]G
BalI 763 TGGCCA TGG^CCA
BbsI 150 GTCTTC GAAGAC(2/6)
BbvCI 13 GCTGAGG CCTCAGC(-5/-2)
BciVI 842 GGATAC GTATCC(6/5)
BfaI 633 CTAG C^TAG
BglI 366 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1008 GCTGAGC GC^T[ACGT]AGC
BpmI 314 CTCCAG CTGGAG(16/14)
Bpu1102I 1008 GCTGAGC GC^T[ACGT]AGC
BpuAI 150 GTCTTC GAAGAC(2/6)
BsaBI 843 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 52 TCCGGA [AT]^CCGG[AT]
BseAI 52 TCCGGA T^CCGGA
BseRI 576 GAGGAG GAGGAG(10/8)
BsgI 1118 CTGCAC GTGCAG(16/14)
BsiEI 24 CGGCCG CG[GA][CT]^CG
BsmFI 96 GGGAC GGGAC(10/14)
BsmI 1058 GAATGC GAATGC(1/-1)
BsoBI 144 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1107 TGTACA T^GTACA
BspEI 52 TCCGGA T^CCGGA
BsrDI 109 GCAATG GCAATG(2/0)
BsrFI 26 GCCGGT [GA]^CCGG[CT]
BsrGI 1107 TGTACA T^GTACA
BstF5I 581 GGATG GGATG(2/0)
BstUI 61 CGCG CG^CG
CelII 1008 GCTGAGC GC^T[ACGT]AGC
CfoI 60 GCGC GCG^C
Cfr10I 26 GCCGGT [GA]^CCGG[CT]
DpnII 849 GATC ^GATC
DraI 441 TTTAAA TTT^AAA
DrdI 141 GACCCCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 838 CCATGG C^C[GA][CT]GG
EagI 24 CGGCCG C^GGCCG
EclXI 24 CGGCCG C^GGCCG
Eco52I 24 CGGCCG C^GGCCG
EcoT14I 838 CCATGG C^C[AT][AT]GG
EcoT22I 1220 ATGCAT ATGCA^T
FokI 581 GGATG GGATG(9/13)
HhaI 60 GCGC GCG^C
HinP1I 60 GCGC G^CGC
HphI 154 TCACC GGTGA(8/7)
MaeI 633 CTAG C^TAG
MamI 843 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 849 GATC ^GATC
MfeI 655 CAATTG C^AATTG
MluNI 763 TGGCCA TGG^CCA
MroI 52 TCCGGA T^CCGGA
MscI 763 TGGCCA TGG^CCA
MunI 655 CAATTG C^AATTG
MvnI 61 CGCG CG^CG
NcoI 838 CCATGG C^CATGG
NdeII 849 GATC ^GATC
NsiI 1220 ATGCAT ATGCA^T
PflFI 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 351 ATTAAT AT^TAAT
PstI 694 CTGCAG CTGCA^G
SacI 159 GAGCTC GAGCT^C
Sau3AI 849 GATC ^GATC
SfcI 694 CTGCAG C^T[GA][CT]AG
SmaI 144 CCCGGG CCC^GGG
SphI 669 GCATGC GCATG^C
SspBI 1107 TGTACA T^GTACA
SspI 329 AATATT AAT^ATT
StyI 838 CCATGG C^C[AT][AT]GG
Tsp45I 1283 GTGAC ^GT[GC]AC
Tth111I 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 144 CCCGGG C^CCGGG
XmaI 144 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 516 GTGCAC G^TGCAC
1182 GTGCAC
ApaI 187 GGGCCC GGGCC^C
650 GGGCCC
ApaLI 516 GTGCAC G^TGCAC
1182 GTGCAC
AvaII 97 GGACC G^G[AT]CC
305 GGTCC
BsmAI 426 GAGAC GTCTC(1/5)
127 GTCTC
BstAPI 33 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1259 GCATGTAGTGC
DraII 186 GGGGCCC [GA]G^G[ACGT]CC[CT]
649 AGGGCCC
EaeI 24 CGGCCG [CT]^GGCC[GA]
763 TGGCCA
EarI 915 GAAGAG CTCTTC(1/4)
1087 GAAGAG
EcoO109I 186 GGGGCCC [GA]G^G[ACGT]CC[CT]
649 AGGGCCC
Ksp632I 915 GAAGAG CTCTTC(1/4)
1087 GAAGAG
MslI 405 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1254 CATGGGCATG
NspI 669 GCATGC [GA]CATG^[CT]
1259 GCATGT
PflMI 243 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
609 CCAGTCAATGG
PleI 312 GACTC GAGTC(4/5)
1176 GACTC
Van91I 243 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
609 CCAGTCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I