################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaHI BsaI BsiWI BsmBI BspDI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MflI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I StuI SwaI XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 76 enzymes) # ################################### AccII 61 CGCG CG^CG AccIII 52 TCCGGA T^CCGGA AflIII 1102 ACGTGT A^C[GA][CT]GT AseI 351 ATTAAT AT^TAAT AsnI 351 ATTAAT AT^TAAT AspI 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 144 CCCGGG C^[CT]CG[GA]G BalI 763 TGGCCA TGG^CCA BbsI 150 GTCTTC GAAGAC(2/6) BbvCI 13 GCTGAGG CCTCAGC(-5/-2) BciVI 842 GGATAC GTATCC(6/5) BfaI 633 CTAG C^TAG BglI 366 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1008 GCTGAGC GC^T[ACGT]AGC BpmI 314 CTCCAG CTGGAG(16/14) Bpu1102I 1008 GCTGAGC GC^T[ACGT]AGC BpuAI 150 GTCTTC GAAGAC(2/6) BsaBI 843 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 52 TCCGGA [AT]^CCGG[AT] BseAI 52 TCCGGA T^CCGGA BseRI 576 GAGGAG GAGGAG(10/8) BsgI 1118 CTGCAC GTGCAG(16/14) BsiEI 24 CGGCCG CG[GA][CT]^CG BsmFI 96 GGGAC GGGAC(10/14) BsmI 1058 GAATGC GAATGC(1/-1) BsoBI 144 CCCGGG C^[CT]CG[GA]G Bsp1407I 1107 TGTACA T^GTACA BspEI 52 TCCGGA T^CCGGA BsrDI 109 GCAATG GCAATG(2/0) BsrFI 26 GCCGGT [GA]^CCGG[CT] BsrGI 1107 TGTACA T^GTACA BstF5I 581 GGATG GGATG(2/0) BstUI 61 CGCG CG^CG CelII 1008 GCTGAGC GC^T[ACGT]AGC CfoI 60 GCGC GCG^C Cfr10I 26 GCCGGT [GA]^CCGG[CT] DpnII 849 GATC ^GATC DraI 441 TTTAAA TTT^AAA DrdI 141 GACCCCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 838 CCATGG C^C[GA][CT]GG EagI 24 CGGCCG C^GGCCG EclXI 24 CGGCCG C^GGCCG Eco52I 24 CGGCCG C^GGCCG EcoT14I 838 CCATGG C^C[AT][AT]GG EcoT22I 1220 ATGCAT ATGCA^T FokI 581 GGATG GGATG(9/13) HhaI 60 GCGC GCG^C HinP1I 60 GCGC G^CGC HphI 154 TCACC GGTGA(8/7) MaeI 633 CTAG C^TAG MamI 843 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 849 GATC ^GATC MfeI 655 CAATTG C^AATTG MluNI 763 TGGCCA TGG^CCA MroI 52 TCCGGA T^CCGGA MscI 763 TGGCCA TGG^CCA MunI 655 CAATTG C^AATTG MvnI 61 CGCG CG^CG NcoI 838 CCATGG C^CATGG NdeII 849 GATC ^GATC NsiI 1220 ATGCAT ATGCA^T PflFI 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 351 ATTAAT AT^TAAT PstI 694 CTGCAG CTGCA^G SacI 159 GAGCTC GAGCT^C Sau3AI 849 GATC ^GATC SfcI 694 CTGCAG C^T[GA][CT]AG SmaI 144 CCCGGG CCC^GGG SphI 669 GCATGC GCATG^C SspBI 1107 TGTACA T^GTACA SspI 329 AATATT AAT^ATT StyI 838 CCATGG C^C[AT][AT]GG Tsp45I 1283 GTGAC ^GT[GC]AC Tth111I 98 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 144 CCCGGG C^CCGGG XmaI 144 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### Alw44I 516 GTGCAC G^TGCAC 1182 GTGCAC ApaI 187 GGGCCC GGGCC^C 650 GGGCCC ApaLI 516 GTGCAC G^TGCAC 1182 GTGCAC AvaII 97 GGACC G^G[AT]CC 305 GGTCC BsmAI 426 GAGAC GTCTC(1/5) 127 GTCTC BstAPI 33 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1259 GCATGTAGTGC DraII 186 GGGGCCC [GA]G^G[ACGT]CC[CT] 649 AGGGCCC EaeI 24 CGGCCG [CT]^GGCC[GA] 763 TGGCCA EarI 915 GAAGAG CTCTTC(1/4) 1087 GAAGAG EcoO109I 186 GGGGCCC [GA]G^G[ACGT]CC[CT] 649 AGGGCCC Ksp632I 915 GAAGAG CTCTTC(1/4) 1087 GAAGAG MslI 405 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1254 CATGGGCATG NspI 669 GCATGC [GA]CATG^[CT] 1259 GCATGT PflMI 243 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 609 CCAGTCAATGG PleI 312 GACTC GAGTC(4/5) 1176 GACTC Van91I 243 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 609 CCAGTCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboII MnlI MseI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I