##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AccII AclI AcsI AgeI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI AviII AvrII BbeI BbrPI
BclI BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AatII 851 GACGTC GACGT^C
Acc65I 1034 GGTACC G^GTACC
AccI 12 GTCTAC GT^[AC][GT]AC
AccIII 122 TCCGGA T^CCGGA
AcyI 851 GACGTC G[GA]^CG[CT]C
AflII 562 CTTAAG C^TTAAG
AflIII 809 ACATGT A^C[GA][CT]GT
AhdI 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 703 GTGCAC G^TGCAC
AlwNI 160 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 703 GTGCAC G^TGCAC
Asp700I 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1034 GGTACC G^GTACC
AspEI 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 726 CCCGGG C^[CT]CG[GA]G
BalI 74 TGGCCA TGG^CCA
BamHI 1014 GGATCC G^GATCC
BanI 1034 GGTACC G^G[CT][GA]CC
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
BciVI 263 GGATAC GTATCC(6/5)
BfrI 562 CTTAAG C^TTAAG
BglI 631 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 969 AGATCT A^GATCT
BsaAI 15 TACGTA [CT]AC^GT[GA]
BsaHI 851 GACGTC G[GA]^CG[CT]C
BsaI 772 GGTCTC GGTCTC(1/5)
BsaWI 122 TCCGGA [AT]^CCGG[AT]
BseAI 122 TCCGGA T^CCGGA
BsoBI 726 CCCGGG C^[CT]CG[GA]G
BspEI 122 TCCGGA T^CCGGA
BspLU11I 809 ACATGT A^CATGT
BspMI 608 ACCTGC ACCTGC(4/8)
BsrI 389 ACTGG ACTGG(1/-1)
BssSI 844 CACGAG CACGAG(-5/-1)
CfoI 68 GCGC GCG^C
DraI 1145 TTTAAA TTT^AAA
DraII 446 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 818 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 74 TGGCCA [CT]^GGCC[GA]
Eam1105I 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 438 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 446 AGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 67 AGCGCC [GA]GCGC^[CT]
HhaI 68 GCGC GCG^C
Hin1I 851 GACGTC G[GA]^CG[CT]C
HinP1I 68 GCGC G^CGC
HincII 813 GTTGAC GT[CT]^[GA]AC
HindII 813 GTTGAC GT[CT]^[GA]AC
KpnI 1034 GGTACC GGTAC^C
MluNI 74 TGGCCA TGG^CCA
MroI 122 TCCGGA T^CCGGA
MscI 74 TGGCCA TGG^CCA
MslI 589 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 632 CCATGG C^CATGG
PflFI 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1067 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 788 CTGCAG CTGCA^G
SacI 32 GAGCTC GAGCT^C
SexAI 1081 ACCTGGT A^CC[AT]GGT
SfcI 788 CTGCAG C^T[GA][CT]AG
SmaI 726 CCCGGG CCC^GGG
SmlI 562 CTTAAG C^T[CT][GA]AG
SnaBI 15 TACGTA TAC^GTA
Tsp509I 501 AATT ^AATT
Tth111I 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1067 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 726 CCCGGG C^CCGGG
XmaI 726 CCCGGG C^CCGGG
XmnI 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 32 GAGCTC G[AT]GC[AT]^C
703 GTGCAC
BanII 32 GAGCTC G[GA]GC[CT]^C
352 GGGCTC
BbsI 364 GTCTTC GAAGAC(2/6)
511 GTCTTC
BcgI 377 GCAGGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
473 GCATCTTGATCG
BcnI 726 CCCGG CC^[GC]GG
727 CCGGG
BfaI 9 CTAG C^TAG
869 CTAG
BpmI 682 CTGGAG CTGGAG(16/14)
947 CTGGAG
BpuAI 364 GTCTTC GAAGAC(2/6)
511 GTCTTC
BsgI 375 GTGCAG GTGCAG(16/14)
1028 GTGCAG
BsiHKAI 32 GAGCTC G[AT]GC[AT]^C
703 GTGCAC
BsmFI 670 GGGAC GGGAC(10/14)
1086 GTCCC
BsrDI 432 CATTGC GCAATG(2/0)
134 GCAATG
BstXI 65 CCAGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
674 CCAGCTGCCTGG
BstYI 969 AGATCT [GA]^GATC[CT]
1014 GGATCC
DsaI 632 CCATGG C^C[GA][CT]GG
1018 CCGTGG
HapII 123 CCGG C^CGG
727 CCGG
HinfI 154 GACTC G^A[ACGT]TC
658 GACTC
HpaII 123 CCGG C^CGG
727 CCGG
HphI 963 GGTGA GGTGA(8/7)
1062 TCACC
MaeI 9 CTAG C^TAG
869 CTAG
MaeII 16 ACGT A^CGT
852 ACGT
MflI 969 AGATCT [GA]^GATC[CT]
1014 GGATCC
MspA1I 675 CAGCTG C[AC]G^C[GT]G
688 CAGCTG
MspI 123 CCGG C^CGG
727 CCGG
NciI 726 CCCGG CC^[GC]GG
727 CCGGG
PleI 154 GACTC GAGTC(4/5)
658 GACTC
PvuII 675 CAGCTG CAG^CTG
688 CAGCTG
StuI 5 AGGCCT AGG^CCT
599 AGGCCT
XhoII 969 AGATCT [GA]^GATC[CT]
1014 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII ItaI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I