################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AccII AclI AcsI AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI AviII AvrII BbeI BbrPI BclI BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HpaI KasI Ksp632I KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI SwaI TfiI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### AatII 851 GACGTC GACGT^C Acc65I 1034 GGTACC G^GTACC AccI 12 GTCTAC GT^[AC][GT]AC AccIII 122 TCCGGA T^CCGGA AcyI 851 GACGTC G[GA]^CG[CT]C AflII 562 CTTAAG C^TTAAG AflIII 809 ACATGT A^C[GA][CT]GT AhdI 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 703 GTGCAC G^TGCAC AlwNI 160 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 703 GTGCAC G^TGCAC Asp700I 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1034 GGTACC G^GTACC AspEI 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 726 CCCGGG C^[CT]CG[GA]G BalI 74 TGGCCA TGG^CCA BamHI 1014 GGATCC G^GATCC BanI 1034 GGTACC G^G[CT][GA]CC BbvCI 129 CCTCAGC CCTCAGC(-5/-2) BciVI 263 GGATAC GTATCC(6/5) BfrI 562 CTTAAG C^TTAAG BglI 631 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 969 AGATCT A^GATCT BsaAI 15 TACGTA [CT]AC^GT[GA] BsaHI 851 GACGTC G[GA]^CG[CT]C BsaI 772 GGTCTC GGTCTC(1/5) BsaWI 122 TCCGGA [AT]^CCGG[AT] BseAI 122 TCCGGA T^CCGGA BsoBI 726 CCCGGG C^[CT]CG[GA]G BspEI 122 TCCGGA T^CCGGA BspLU11I 809 ACATGT A^CATGT BspMI 608 ACCTGC ACCTGC(4/8) BsrI 389 ACTGG ACTGG(1/-1) BssSI 844 CACGAG CACGAG(-5/-1) CfoI 68 GCGC GCG^C DraI 1145 TTTAAA TTT^AAA DraII 446 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 818 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 74 TGGCCA [CT]^GGCC[GA] Eam1105I 388 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 438 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 446 AGGGCCT [GA]G^G[ACGT]CC[CT] HaeII 67 AGCGCC [GA]GCGC^[CT] HhaI 68 GCGC GCG^C Hin1I 851 GACGTC G[GA]^CG[CT]C HinP1I 68 GCGC G^CGC HincII 813 GTTGAC GT[CT]^[GA]AC HindII 813 GTTGAC GT[CT]^[GA]AC KpnI 1034 GGTACC GGTAC^C MluNI 74 TGGCCA TGG^CCA MroI 122 TCCGGA T^CCGGA MscI 74 TGGCCA TGG^CCA MslI 589 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 632 CCATGG C^CATGG PflFI 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1067 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 788 CTGCAG CTGCA^G SacI 32 GAGCTC GAGCT^C SexAI 1081 ACCTGGT A^CC[AT]GGT SfcI 788 CTGCAG C^T[GA][CT]AG SmaI 726 CCCGGG CCC^GGG SmlI 562 CTTAAG C^T[CT][GA]AG SnaBI 15 TACGTA TAC^GTA Tsp509I 501 AATT ^AATT Tth111I 1080 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1067 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 726 CCCGGG C^CCGGG XmaI 726 CCCGGG C^CCGGG XmnI 507 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 32 GAGCTC G[AT]GC[AT]^C 703 GTGCAC BanII 32 GAGCTC G[GA]GC[CT]^C 352 GGGCTC BbsI 364 GTCTTC GAAGAC(2/6) 511 GTCTTC BcgI 377 GCAGGATGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 473 GCATCTTGATCG BcnI 726 CCCGG CC^[GC]GG 727 CCGGG BfaI 9 CTAG C^TAG 869 CTAG BpmI 682 CTGGAG CTGGAG(16/14) 947 CTGGAG BpuAI 364 GTCTTC GAAGAC(2/6) 511 GTCTTC BsgI 375 GTGCAG GTGCAG(16/14) 1028 GTGCAG BsiHKAI 32 GAGCTC G[AT]GC[AT]^C 703 GTGCAC BsmFI 670 GGGAC GGGAC(10/14) 1086 GTCCC BsrDI 432 CATTGC GCAATG(2/0) 134 GCAATG BstXI 65 CCAGCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 674 CCAGCTGCCTGG BstYI 969 AGATCT [GA]^GATC[CT] 1014 GGATCC DsaI 632 CCATGG C^C[GA][CT]GG 1018 CCGTGG HapII 123 CCGG C^CGG 727 CCGG HinfI 154 GACTC G^A[ACGT]TC 658 GACTC HpaII 123 CCGG C^CGG 727 CCGG HphI 963 GGTGA GGTGA(8/7) 1062 TCACC MaeI 9 CTAG C^TAG 869 CTAG MaeII 16 ACGT A^CGT 852 ACGT MflI 969 AGATCT [GA]^GATC[CT] 1014 GGATCC MspA1I 675 CAGCTG C[AC]G^C[GT]G 688 CAGCTG MspI 123 CCGG C^CGG 727 CCGG NciI 726 CCCGG CC^[GC]GG 727 CCGGG PleI 154 GACTC GAGTC(4/5) 658 GACTC PvuII 675 CAGCTG CAG^CTG 688 CAGCTG StuI 5 AGGCCT AGG^CCT 599 AGGCCT XhoII 969 AGATCT [GA]^GATC[CT] 1014 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI FokI HaeIII ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I