##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BbeI BbrPI
BbsI BbvCI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FauI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MslI MunI NarI
NciI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 123 CGCG CG^CG
AflIII 1152 ACACGT A^C[GA][CT]GT
AocI 265 CCTGAGG CC^T[ACGT]AGG
Aor51HI 356 AGCGCT AGC^GCT
AseI 250 ATTAAT AT^TAAT
AsnI 250 ATTAAT AT^TAAT
BalI 56 TGGCCA TGG^CCA
BamHI 492 GGATCC G^GATCC
BanI 589 GGCACC G^G[CT][GA]CC
BciVI 261 GTATCC GTATCC(6/5)
BclI 652 TGATCA T^GATCA
BsaBI 138 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 887 GCATTC GAATGC(1/-1)
BssSI 1277 CACGAG CACGAG(-5/-1)
BstUI 123 CGCG CG^CG
BstXI 1150 CCACACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 265 CCTGAGG CC^T[ACGT]AGG
DraI 1292 TTTAAA TTT^AAA
DraIII 159 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 356 AGCGCT AGC^GCT
Eco81I 265 CCTGAGG CC^T[ACGT]AGG
EcoT22I 885 ATGCAT ATGCA^T
FbaI 652 TGATCA T^GATCA
HaeII 356 AGCGCT [GA]GCGC^[CT]
HindIII 244 AAGCTT A^AGCTT
MamI 138 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 56 TGGCCA TGG^CCA
MscI 56 TGGCCA TGG^CCA
MvnI 123 CGCG CG^CG
NaeI 613 GCCGGC GCC^GGC
NcoI 1023 CCATGG C^CATGG
NgoMIV 613 GCCGGC G^CCGGC
NsiI 885 ATGCAT ATGCA^T
PleI 1006 GAGTC GAGTC(4/5)
PpuMI 66 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 250 ATTAAT AT^TAAT
SapI 480 GCTCTTC GCTCTTC(1/4)
SbfI 342 CCTGCAGG CCTGCA^GG
SmlI 220 CTCAAG C^T[CT][GA]AG
Sse8387I 342 CCTGCAGG CCTGCA^GG
TaqI 304 TCGA T^CGA
TfiI 1014 GATTC G^A[AT]TC
TthHB8I 304 TCGA T^CGA
XcmI 183 CCAGCCCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 124 GCGG CCGC(-3/-1)
432 GCGG
AfaI 117 GTAC GT^AC
1143 GTAC
AlwNI 337 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
798 CAGGGCCTG
BanII 478 GGGCTC G[GA]GC[CT]^C
734 GAGCCC
BfaI 1002 CTAG C^TAG
1172 CTAG
BglII 329 AGATCT A^GATCT
1303 AGATCT
BpmI 1124 CTCCAG CTGGAG(16/14)
130 CTGGAG
BsrFI 613 GCCGGC [GA]^CCGG[CT]
622 ACCGGC
CfoI 1 GCGC GCG^C
357 GCGC
Cfr10I 613 GCCGGC [GA]^CCGG[CT]
622 ACCGGC
EaeI 56 TGGCCA [CT]^GGCC[GA]
195 TGGCCG
HapII 614 CCGG C^CGG
623 CCGG
HgaI 23 GACGC GACGC(5/10)
121 GACGC
HhaI 1 GCGC GCG^C
357 GCGC
HinP1I 1 GCGC G^CGC
357 GCGC
HinfI 1006 GAGTC G^A[ACGT]TC
1014 GATTC
HpaII 614 CCGG C^CGG
623 CCGG
MaeI 1002 CTAG C^TAG
1172 CTAG
MaeIII 787 GTCAC ^GT[ACGT]AC
1120 GTCAC
MspI 614 CCGG C^CGG
623 CCGG
PstI 343 CTGCAG CTGCA^G
1083 CTGCAG
RsaI 117 GTAC GT^AC
1143 GTAC
StuI 296 AGGCCT AGG^CCT
1062 AGGCCT
Tsp45I 787 GTCAC ^GT[GC]AC
1120 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AluI AlwI AvaII BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PflMI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp509I TspRI Van91I XhoII