##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AccII AccIII AciI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT14I FauI FbaI FseI FspI
HaeII HgaI HhaI HinP1I HincII
HindII HindIII HpaI KasI Ksp632I
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 423 GACGTC GACGT^C
Acc65I 106 GGTACC G^GTACC
AccI 375 GTCTAC GT^[AC][GT]AC
AcyI 423 GACGTC G[GA]^CG[CT]C
AflIII 727 ACATGT A^C[GA][CT]GT
AocI 837 CCTCAGG CC^T[ACGT]AGG
Asp718I 106 GGTACC G^GTACC
AspHI 873 GAGCTC G[AT]GC[AT]^C
AspI 342 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 758 GGTCC G^G[AT]CC
BanI 106 GGTACC G^G[CT][GA]CC
BbvCI 785 GCTGAGG CCTCAGC(-5/-2)
BsaHI 423 GACGTC G[GA]^CG[CT]C
BsaI 455 GAGACC GGTCTC(1/5)
BsgI 142 GTGCAG GTGCAG(16/14)
BsiHKAI 873 GAGCTC G[AT]GC[AT]^C
BsmFI 576 GGGAC GGGAC(10/14)
Bsp1407I 1083 TGTACA T^GTACA
BspLU11I 727 ACATGT A^CATGT
BsrGI 1083 TGTACA T^GTACA
BsrI 608 ACTGG ACTGG(1/-1)
BstAPI 909 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 623 CCAGATAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 736 AGATCC [GA]^GATC[CT]
Bsu36I 837 CCTCAGG CC^T[ACGT]AGG
DraII 757 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 837 CCTCAGG CC^T[ACGT]AGG
EcoO109I 757 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 169 GATATC GAT^ATC
EcoT22I 93 ATGCAT ATGCA^T
Hin1I 423 GACGTC G[GA]^CG[CT]C
KpnI 106 GGTACC GGTAC^C
MflI 736 AGATCC [GA]^GATC[CT]
MspA1I 145 CAGCTG C[AC]G^C[GT]G
MwoI 909 GCAAAAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 843 GCTAGC G^CTAGC
NsiI 93 ATGCAT ATGCA^T
PflFI 342 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 708 GACTC GAGTC(4/5)
PpuMI 757 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 145 CAGCTG CAG^CTG
SacI 873 GAGCTC GAGCT^C
SphI 528 GCATGC GCATG^C
SspBI 1083 TGTACA T^GTACA
TfiI 24 GAATC G^A[AT]TC
Tsp45I 945 GTGAC ^GT[GC]AC
Tsp509I 361 AATT ^AATT
Tth111I 342 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 736 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 737 GATCC GGATC(4/5)
418 GGATC
AlwNI 574 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1076 CAGGCTCTG
BalI 277 TGGCCA TGG^CCA
1072 TGGCCA
BanII 50 GGGCTC G[GA]GC[CT]^C
873 GAGCTC
BbsI 705 GAAGAC GAAGAC(2/6)
348 GTCTTC
BpuAI 705 GAAGAC GAAGAC(2/6)
348 GTCTTC
BspHI 711 TCATGA T^CATGA
769 TCATGA
EaeI 277 TGGCCA [CT]^GGCC[GA]
1072 TGGCCA
HinfI 24 GAATC G^A[ACGT]TC
708 GACTC
HphI 447 TCACC GGTGA(8/7)
537 TCACC
MaeII 373 ACGT A^CGT
424 ACGT
MaeIII 298 GTTAC ^GT[ACGT]AC
945 GTGAC
MluNI 277 TGGCCA TGG^CCA
1072 TGGCCA
MscI 277 TGGCCA TGG^CCA
1072 TGGCCA
MslI 258 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
936 CATGCCCATG
RcaI 711 TCATGA T^CATGA
769 TCATGA
SfcI 831 CTGTAG C^T[GA][CT]AG
1056 CTATAG
TaqI 38 TCGA T^CGA
421 TCGA
TspRI 140 CAGTG CA[GC]TG[ACGT][ACGT]^
635 CACTG
TthHB8I 38 TCGA T^CGA
421 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI BbvI BcnI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeI MboI MboII MnlI
MseI MspI MvaI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI