################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BglI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI HinP1I HindIII HpaI HpaII KasI KpnI KspI MfeI MflI MluI MroI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 1088 GACGTC GACGT^C AccI 627 GTCTAC GT^[AC][GT]AC AccII 540 CGCG CG^CG AciI 539 CCGC CCGC(-3/-1) AcyI 1088 GACGTC G[GA]^CG[CT]C AfaI 899 GTAC GT^AC AflII 1228 CTTAAG C^TTAAG AflIII 631 ACATGT A^C[GA][CT]GT AhdI 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 426 GATCC GGATC(4/5) AlwNI 968 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 767 ATTAAT AT^TAAT AsnI 767 ATTAAT AT^TAAT AspEI 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 543 GTGCTC G[AT]GC[AT]^C BalI 566 TGGCCA TGG^CCA BbvCI 927 CCTCAGC CCTCAGC(-5/-2) BfrI 1228 CTTAAG C^TTAAG BlpI 456 GCTCAGC GC^T[ACGT]AGC Bpu1102I 456 GCTCAGC GC^T[ACGT]AGC BsaBI 420 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1088 GACGTC G[GA]^CG[CT]C BsaI 936 GGTCTC GGTCTC(1/5) BsaJI 907 CCCTGG C^C[ACGT][ACGT]GG BsgI 153 GTGCAG GTGCAG(16/14) BsiHKAI 543 GTGCTC G[AT]GC[AT]^C BsmFI 619 GTCCC GGGAC(10/14) Bsp1407I 898 TGTACA T^GTACA BspLU11I 631 ACATGT A^CATGT BsrGI 898 TGTACA T^GTACA BstAPI 49 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 452 GGATG GGATG(2/0) BstUI 540 CGCG CG^CG Cac8I 541 GCGTGC GC[ACGT]^[ACGT]GC CelII 456 GCTCAGC GC^T[ACGT]AGC DraI 1131 TTTAAA TTT^AAA EaeI 566 TGGCCA [CT]^GGCC[GA] Eam1105I 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1067 GAAGAG CTCTTC(1/4) EcoRI 596 GAATTC G^AATTC FokI 452 GGATG GGATG(9/13) HgaI 625 GCGTC GACGC(5/10) Hin1I 1088 GACGTC G[GA]^CG[CT]C HincII 863 GTTGAC GT[CT]^[GA]AC HindII 863 GTTGAC GT[CT]^[GA]AC Ksp632I 1067 GAAGAG CTCTTC(1/4) MaeII 1089 ACGT A^CGT MamI 420 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 566 TGGCCA TGG^CCA MscI 566 TGGCCA TGG^CCA MspA1I 614 CAGCTG C[AC]G^C[GT]G MvnI 540 CGCG CG^CG PleI 191 GACTC GAGTC(4/5) PshAI 1088 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 767 ATTAAT AT^TAAT PstI 773 CTGCAG CTGCA^G PvuII 614 CAGCTG CAG^CTG RsaI 899 GTAC GT^AC SfcI 773 CTGCAG C^T[GA][CT]AG SpeI 1222 ACTAGT A^CTAGT SspBI 898 TGTACA T^GTACA XcmI 569 CCACTAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaII 322 GGTCC G^G[AT]CC 610 GGACC BssKI 521 CCAGG ^CC[ACGT]GG 908 CCTGG BstNI 521 CCAGG CC^[AT]GG 908 CCTGG Cfr13I 322 GGTCC G^G[ACGT]CC 610 GGACC DpnII 426 GATC ^GATC 1240 GATC EcoRII 521 CCAGG ^CC[AT]GG 908 CCTGG HphI 396 GGTGA GGTGA(8/7) 918 GGTGA MboI 426 GATC ^GATC 1240 GATC MvaI 521 CCAGG CC^[AT]GG 908 CCTGG MwoI 49 GCAGCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 616 GCTGTCCCAGC NdeII 426 GATC ^GATC 1240 GATC NlaIV 321 GGGTCC GG[ACGT]^[ACGT]CC 517 GGAGCC NspI 631 ACATGT [GA]CATG^[CT] 953 ACATGC Sau3AI 426 GATC ^GATC 1240 GATC Sau96I 322 GGTCC G^G[ACGT]CC 610 GGACC ScrFI 521 CCAGG CC^[ACGT]GG 908 CCTGG SmlI 509 CTTGAG C^T[CT][GA]AG 1228 CTTAAG StuI 494 AGGCCT AGG^CCT 871 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AcsI AluI ApoI BbvI BfaI BmyI BpmI BseRI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI DdeI Fnu4HI HaeIII HinfI ItaI MaeI MaeIII MboII MnlI MseI NlaIII SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI