##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BclI
BcnI BglI BglII BlnI BpuAI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MfeI MflI MluI
MroI MslI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 1088 GACGTC GACGT^C
AccI 627 GTCTAC GT^[AC][GT]AC
AccII 540 CGCG CG^CG
AciI 539 CCGC CCGC(-3/-1)
AcyI 1088 GACGTC G[GA]^CG[CT]C
AfaI 899 GTAC GT^AC
AflII 1228 CTTAAG C^TTAAG
AflIII 631 ACATGT A^C[GA][CT]GT
AhdI 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 426 GATCC GGATC(4/5)
AlwNI 968 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 767 ATTAAT AT^TAAT
AsnI 767 ATTAAT AT^TAAT
AspEI 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 543 GTGCTC G[AT]GC[AT]^C
BalI 566 TGGCCA TGG^CCA
BbvCI 927 CCTCAGC CCTCAGC(-5/-2)
BfrI 1228 CTTAAG C^TTAAG
BlpI 456 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 456 GCTCAGC GC^T[ACGT]AGC
BsaBI 420 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1088 GACGTC G[GA]^CG[CT]C
BsaI 936 GGTCTC GGTCTC(1/5)
BsaJI 907 CCCTGG C^C[ACGT][ACGT]GG
BsgI 153 GTGCAG GTGCAG(16/14)
BsiHKAI 543 GTGCTC G[AT]GC[AT]^C
BsmFI 619 GTCCC GGGAC(10/14)
Bsp1407I 898 TGTACA T^GTACA
BspLU11I 631 ACATGT A^CATGT
BsrGI 898 TGTACA T^GTACA
BstAPI 49 GCAGCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 452 GGATG GGATG(2/0)
BstUI 540 CGCG CG^CG
Cac8I 541 GCGTGC GC[ACGT]^[ACGT]GC
CelII 456 GCTCAGC GC^T[ACGT]AGC
DraI 1131 TTTAAA TTT^AAA
EaeI 566 TGGCCA [CT]^GGCC[GA]
Eam1105I 611 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1067 GAAGAG CTCTTC(1/4)
EcoRI 596 GAATTC G^AATTC
FokI 452 GGATG GGATG(9/13)
HgaI 625 GCGTC GACGC(5/10)
Hin1I 1088 GACGTC G[GA]^CG[CT]C
HincII 863 GTTGAC GT[CT]^[GA]AC
HindII 863 GTTGAC GT[CT]^[GA]AC
Ksp632I 1067 GAAGAG CTCTTC(1/4)
MaeII 1089 ACGT A^CGT
MamI 420 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MspA1I 614 CAGCTG C[AC]G^C[GT]G
MvnI 540 CGCG CG^CG
PleI 191 GACTC GAGTC(4/5)
PshAI 1088 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 767 ATTAAT AT^TAAT
PstI 773 CTGCAG CTGCA^G
PvuII 614 CAGCTG CAG^CTG
RsaI 899 GTAC GT^AC
SfcI 773 CTGCAG C^T[GA][CT]AG
SpeI 1222 ACTAGT A^CTAGT
SspBI 898 TGTACA T^GTACA
XcmI 569 CCACTAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 322 GGTCC G^G[AT]CC
610 GGACC
BssKI 521 CCAGG ^CC[ACGT]GG
908 CCTGG
BstNI 521 CCAGG CC^[AT]GG
908 CCTGG
Cfr13I 322 GGTCC G^G[ACGT]CC
610 GGACC
DpnII 426 GATC ^GATC
1240 GATC
EcoRII 521 CCAGG ^CC[AT]GG
908 CCTGG
HphI 396 GGTGA GGTGA(8/7)
918 GGTGA
MboI 426 GATC ^GATC
1240 GATC
MvaI 521 CCAGG CC^[AT]GG
908 CCTGG
MwoI 49 GCAGCCGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
616 GCTGTCCCAGC
NdeII 426 GATC ^GATC
1240 GATC
NlaIV 321 GGGTCC GG[ACGT]^[ACGT]CC
517 GGAGCC
NspI 631 ACATGT [GA]CATG^[CT]
953 ACATGC
Sau3AI 426 GATC ^GATC
1240 GATC
Sau96I 322 GGTCC G^G[ACGT]CC
610 GGACC
ScrFI 521 CCAGG CC^[ACGT]GG
908 CCTGG
SmlI 509 CTTGAG C^T[CT][GA]AG
1228 CTTAAG
StuI 494 AGGCCT AGG^CCT
871 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BmyI BpmI BseRI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI DdeI
Fnu4HI HaeIII HinfI ItaI MaeI
MaeIII MboII MnlI MseI NlaIII
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI