##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I ClaI
CpoI DpnII DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MamI MboI MfeI
MflI MluI MroI MslI MunI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 1085 ACATGT A^C[GA][CT]GT
AhdI 73 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1630 CCTGAGG CC^T[ACGT]AGG
AspEI 73 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 737 GGTGCC G^G[CT][GA]CC
BbsI 241 GTCTTC GAAGAC(2/6)
BbvCI 54 CCTCAGC CCTCAGC(-5/-2)
BciVI 1721 GTATCC GTATCC(6/5)
BglI 1212 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1207 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1207 GCTCAGC GC^T[ACGT]AGC
BpuAI 241 GTCTTC GAAGAC(2/6)
BseRI 1740 GAGGAG GAGGAG(10/8)
BsgI 968 CTGCAC GTGCAG(16/14)
BsmBI 552 GAGACG CGTCTC(1/5)
BsmFI 344 GGGAC GGGAC(10/14)
Bsp1407I 123 TGTACA T^GTACA
BspLU11I 1085 ACATGT A^CATGT
BspMI 324 GCAGGT ACCTGC(4/8)
BsrDI 1163 CATTGC GCAATG(2/0)
BsrFI 582 GCCGGT [GA]^CCGG[CT]
BsrGI 123 TGTACA T^GTACA
BstAPI 1233 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1630 CCTGAGG CC^T[ACGT]AGG
CelII 1207 GCTCAGC GC^T[ACGT]AGC
Cfr10I 582 GCCGGT [GA]^CCGG[CT]
DraI 1512 TTTAAA TTT^AAA
DrdI 1675 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 73 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1630 CCTGAGG CC^T[ACGT]AGG
EcoRI 1503 GAATTC G^AATTC
EcoRV 304 GATATC GAT^ATC
EcoT14I 18 CCTTGG C^C[AT][AT]GG
FauI 1626 CCCGC CCCGC(4/6)
HgaI 50 GCGTC GACGC(5/10)
HpaI 1151 GTTAAC GTT^AAC
MaeII 717 ACGT A^CGT
PshAI 45 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1677 CTGCAG CTGCA^G
SfcI 1677 CTGCAG C^T[GA][CT]AG
SmlI 89 CTTGAG C^T[CT][GA]AG
SspBI 123 TGTACA T^GTACA
StyI 18 CCTTGG C^C[AT][AT]GG
TaqI 308 TCGA T^CGA
TthHB8I 308 TCGA T^CGA
XbaI 1026 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 138 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1680 CAGGGTCTG
AseI 799 ATTAAT AT^TAAT
905 ATTAAT
AsnI 799 ATTAAT AT^TAAT
905 ATTAAT
BanII 28 GAGCTC G[GA]GC[CT]^C
1171 GAGCTC
BpmI 470 CTCCAG CTGGAG(16/14)
1795 CTCCAG
BsaI 1260 GGTCTC GGTCTC(1/5)
1785 GGTCTC
BssSI 549 CACGAG CACGAG(-5/-1)
693 CACGAG
DraII 527 AGGACCT [GA]G^G[ACGT]CC[CT]
936 GGGTCCT
DsaI 2 CCACGG C^C[GA][CT]GG
278 CCACGG
EarI 1788 CTCTTC CTCTTC(1/4)
63 GAAGAG
EcoNI 592 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1733 CCTCCCAGAGG
EcoO109I 527 AGGACCT [GA]G^G[ACGT]CC[CT]
936 GGGTCCT
HaeII 625 GGCGCT [GA]GCGC^[CT]
988 GGCGCT
HapII 70 CCGG C^CGG
583 CCGG
HincII 1105 GTCAAC GT[CT]^[GA]AC
1151 GTTAAC
HindII 1105 GTCAAC GT[CT]^[GA]AC
1151 GTTAAC
HpaII 70 CCGG C^CGG
583 CCGG
Ksp632I 1788 CTCTTC CTCTTC(1/4)
63 GAAGAG
MspI 70 CCGG C^CGG
583 CCGG
NspI 226 ACATGC [GA]CATG^[CT]
1085 ACATGT
PpuMI 527 AGGACCT [GA]G^G[AT]CC[CT]
936 GGGTCCT
PshBI 799 ATTAAT AT^TAAT
905 ATTAAT
SacI 28 GAGCTC GAGCT^C
1171 GAGCTC
SfaNI 99 GCATC GCATC(5/9)
132 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BalI BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI EaeI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboII MluNI MnlI MscI MseI
MspA1I MvaI MvnI MwoI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI Tru9I TseI Tsp45I Tsp509I
TspRI