##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvrII BalI BbeI BbrPI BbsI
BbvCI BcgI BcnI BglI BglII
BlnI BpmI BpuAI BsaBI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII BstAPI
BstBI ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I FauI FseI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MboII MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I SspI StuI StyI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AccII 930 CGCG CG^CG
AflII 1070 CTTAAG C^TTAAG
AgeI 237 ACCGGT A^CCGGT
Alw44I 904 GTGCAC G^TGCAC
AlwNI 1020 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 998 CCTCAGG CC^T[ACGT]AGG
ApaI 279 GGGCCC GGGCC^C
ApaLI 904 GTGCAC G^TGCAC
Asp700I 1060 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 217 GGACC G^G[AT]CC
AviII 324 TGCGCA TGC^GCA
BamHI 166 GGATCC G^GATCC
BanI 727 GGTGCC G^G[CT][GA]CC
BanII 279 GGGCCC G[GA]GC[CT]^C
BclI 1272 TGATCA T^GATCA
BfrI 1070 CTTAAG C^TTAAG
BlpI 965 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 965 GCTGAGC GC^T[ACGT]AGC
BsaAI 371 TACGTG [CT]AC^GT[GA]
BsaWI 237 ACCGGT [AT]^CCGG[AT]
BseRI 920 GAGGAG GAGGAG(10/8)
BsgI 138 CTGCAC GTGCAG(16/14)
BsiYI 973 CCCAGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 973 CCCAGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 923 GAGAC GTCTC(1/5)
BsmI 1166 GCATTC GAATGC(1/-1)
BsrFI 237 ACCGGT [GA]^CCGG[CT]
BssSI 893 CTCGTG CACGAG(-5/-1)
Bst1107I 243 GTATAC GTA^TAC
BstF5I 941 CATCC GGATG(2/0)
BstUI 930 CGCG CG^CG
BstXI 269 CCACTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 166 GGATCC [GA]^GATC[CT]
BstZ17I 243 GTATAC GTA^TAC
Bsu36I 998 CCTCAGG CC^T[ACGT]AGG
CelII 965 GCTGAGC GC^T[ACGT]AGC
Cfr10I 237 ACCGGT [GA]^CCGG[CT]
DraII 935 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 173 CGGCCA [CT]^GGCC[GA]
Eco81I 998 CCTCAGG CC^T[ACGT]AGG
EcoO109I 935 AGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 1272 TGATCA T^GATCA
FokI 941 CATCC GGATG(9/13)
FspI 324 TGCGCA TGC^GCA
HapII 238 CCGG C^CGG
HincII 540 GTCAAC GT[CT]^[GA]AC
HindII 540 GTCAAC GT[CT]^[GA]AC
HpaII 238 CCGG C^CGG
MaeII 372 ACGT A^CGT
MflI 166 GGATCC [GA]^GATC[CT]
MslI 1167 CATTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 238 CCGG C^CGG
MvnI 930 CGCG CG^CG
PinAI 237 ACCGGT A^CCGGT
PleI 983 GACTC GAGTC(4/5)
PstI 732 CTGCAG CTGCA^G
PvuII 105 CAGCTG CAG^CTG
ScaI 1008 AGTACT AGT^ACT
SfcI 732 CTGCAG C^T[GA][CT]AG
SgrAI 236 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1070 CTTAAG C^T[CT][GA]AG
SpeI 806 ACTAGT A^CTAGT
SphI 321 GCATGC GCATG^C
SwaI 570 ATTTAAAT ATTT^AAAT
TfiI 94 GAATC G^A[AT]TC
XcmI 1163 CCAGCATTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 166 GGATCC [GA]^GATC[CT]
XmnI 1060 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 243 GTATAC GT^[AC][GT]AC
714 GTAGAC
AciI 721 GCGG CCGC(-3/-1)
931 GCGG
AcsI 568 AAATTT [GA]^AATT[CT]
650 AAATTT
AlwI 167 GATCC GGATC(4/5)
166 GGATC
ApoI 568 AAATTT [GA]^AATT[CT]
650 AAATTT
AspHI 904 GTGCAC G[AT]GC[AT]^C
968 GAGCAC
BbvI 306 GCAGC GCAGC(8/12)
994 GCAGC
BciVI 1028 GGATAC GTATCC(6/5)
477 GTATCC
BsiHKAI 904 GTGCAC G[AT]GC[AT]^C
968 GAGCAC
Bsp1407I 1110 TGTACA T^GTACA
1144 TGTACA
BspMI 116 ACCTGC ACCTGC(4/8)
136 ACCTGC
BsrGI 1110 TGTACA T^GTACA
1144 TGTACA
BstEII 112 GGTCACC G^GT[ACGT]ACC
429 GGTGACC
BstPI 112 GGTCACC G^GT[ACGT]ACC
429 GGTGACC
CfoI 325 GCGC GCG^C
929 GCGC
EcoO65I 112 GGTCACC G^GT[ACGT]ACC
429 GGTGACC
EcoT22I 152 ATGCAT ATGCA^T
453 ATGCAT
Fnu4HI 306 GCAGC GC^[ACGT]GC
994 GCAGC
HhaI 325 GCGC GCG^C
929 GCGC
HinP1I 325 GCGC G^CGC
929 GCGC
HinfI 94 GAATC G^A[ACGT]TC
983 GACTC
ItaI 306 GCAGC GC^[ACGT]GC
994 GCAGC
MspA1I 105 CAGCTG C[AC]G^C[GT]G
719 CAGCGG
MwoI 22 GCCAGGCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
721 GCGGTAGGTGC
NsiI 152 ATGCAT ATGCA^T
453 ATGCAT
NspI 321 GCATGC [GA]CATG^[CT]
455 GCATGT
SspBI 1110 TGTACA T^GTACA
1144 TGTACA
TseI 306 GCAGC G^C[AT]GC
994 GCAGC
Tsp45I 113 GTCAC ^GT[GC]AC
430 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BfaI BmyI BsaJI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
HaeIII HphI MaeI MaeIII MboI
MnlI MseI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I Tsp509I TspRI