################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BfrI BglI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 615 GGTACC G^GTACC AcyI 5 GACGCC G[GA]^CG[CT]C AocI 1078 CCTGAGG CC^T[ACGT]AGG Asp718I 615 GGTACC G^GTACC AvaII 1207 GGACC G^G[AT]CC BalI 717 TGGCCA TGG^CCA BanI 615 GGTACC G^G[CT][GA]CC BbrPI 575 CACGTG CAC^GTG BbsI 580 GAAGAC GAAGAC(2/6) BbvCI 1471 CCTCAGC CCTCAGC(-5/-2) BcgI 92 CGACAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 337 GGATAC GTATCC(6/5) BclI 1194 TGATCA T^GATCA BglII 633 AGATCT A^GATCT BpuAI 580 GAAGAC GAAGAC(2/6) BsaHI 5 GACGCC G[GA]^CG[CT]C BsaI 595 GGTCTC GGTCTC(1/5) BsaWI 584 ACCGGA [AT]^CCGG[AT] BsgI 1184 GTGCAG GTGCAG(16/14) BsmBI 398 CGTCTC CGTCTC(1/5) BsmI 421 GAATGC GAATGC(1/-1) BsrBI 75 CCGCTC CCGCTC(-3/-3) Bsu36I 1078 CCTGAGG CC^T[ACGT]AGG EaeI 717 TGGCCA [CT]^GGCC[GA] Eco81I 1078 CCTGAGG CC^T[ACGT]AGG EcoT14I 1285 CCTTGG C^C[AT][AT]GG EcoT22I 966 ATGCAT ATGCA^T FbaI 1194 TGATCA T^GATCA Hin1I 5 GACGCC G[GA]^CG[CT]C HincII 1403 GTTGAC GT[CT]^[GA]AC HindII 1403 GTTGAC GT[CT]^[GA]AC KpnI 615 GGTACC GGTAC^C MaeIII 524 GTGAC ^GT[ACGT]AC MluNI 717 TGGCCA TGG^CCA MscI 717 TGGCCA TGG^CCA NsiI 966 ATGCAT ATGCA^T NspI 964 ACATGC [GA]CATG^[CT] PaeR7I 1223 CTCGAG C^TCGAG PmaCI 575 CACGTG CAC^GTG PmlI 575 CACGTG CAC^GTG PstI 1097 CTGCAG CTGCA^G PvuII 1115 CAGCTG CAG^CTG SacI 1560 GAGCTC GAGCT^C SmaI 53 CCCGGG CCC^GGG StyI 1285 CCTTGG C^C[AT][AT]GG TfiI 301 GATTC G^A[AT]TC Tsp45I 524 GTGAC ^GT[GC]AC XbaI 856 TCTAGA T^CTAGA XcmI 1377 CCAAACAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1223 CTCGAG C^TCGAG XmaCI 53 CCCGGG C^CCGGG XmaI 53 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AhdI 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1484 GACATGAAGTC ApaI 19 GGGCCC GGGCC^C 71 GGGCCC Asp700I 1007 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1234 GAAGCTGTTC AspEI 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1484 GACATGAAGTC AspHI 1253 GAGCAC G[AT]GC[AT]^C 1560 GAGCTC AvaI 53 CCCGGG C^[CT]CG[GA]G 1223 CTCGAG BsaAI 575 CACGTG [CT]AC^GT[GA] 1198 CACGTA BsaBI 701 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1189 GATCCTGATC BsiHKAI 1253 GAGCAC G[AT]GC[AT]^C 1560 GAGCTC BsoBI 53 CCCGGG C^[CT]CG[GA]G 1223 CTCGAG BsrI 1456 ACTGG ACTGG(1/-1) 141 CCAGT DraII 18 GGGGCCC [GA]G^G[ACGT]CC[CT] 474 AGGCCCT Eam1105I 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1484 GACATGAAGTC EcoO109I 18 GGGGCCC [GA]G^G[ACGT]CC[CT] 474 AGGCCCT EcoRV 356 GATATC GAT^ATC 911 GATATC MamI 701 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1189 GATCCTGATC MseI 462 TTAA T^TAA 1625 TTAA PleI 329 GACTC GAGTC(4/5) 1129 GAGTC SfcI 234 CTATAG C^T[GA][CT]AG 1097 CTGCAG TaqI 444 TCGA T^CGA 1224 TCGA Tru9I 462 TTAA T^TAA 1625 TTAA TthHB8I 444 TCGA T^CGA 1224 TCGA XmnI 1007 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1234 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TseI Tsp509I TspRI XhoII