##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
AspI AviII AvrII BamHI BbeI
BfrI BglI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FseI FspI HaeII HindIII
HpaI KasI KspI MfeI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 615 GGTACC G^GTACC
AcyI 5 GACGCC G[GA]^CG[CT]C
AocI 1078 CCTGAGG CC^T[ACGT]AGG
Asp718I 615 GGTACC G^GTACC
AvaII 1207 GGACC G^G[AT]CC
BalI 717 TGGCCA TGG^CCA
BanI 615 GGTACC G^G[CT][GA]CC
BbrPI 575 CACGTG CAC^GTG
BbsI 580 GAAGAC GAAGAC(2/6)
BbvCI 1471 CCTCAGC CCTCAGC(-5/-2)
BcgI 92 CGACAACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 337 GGATAC GTATCC(6/5)
BclI 1194 TGATCA T^GATCA
BglII 633 AGATCT A^GATCT
BpuAI 580 GAAGAC GAAGAC(2/6)
BsaHI 5 GACGCC G[GA]^CG[CT]C
BsaI 595 GGTCTC GGTCTC(1/5)
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BsgI 1184 GTGCAG GTGCAG(16/14)
BsmBI 398 CGTCTC CGTCTC(1/5)
BsmI 421 GAATGC GAATGC(1/-1)
BsrBI 75 CCGCTC CCGCTC(-3/-3)
Bsu36I 1078 CCTGAGG CC^T[ACGT]AGG
EaeI 717 TGGCCA [CT]^GGCC[GA]
Eco81I 1078 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1285 CCTTGG C^C[AT][AT]GG
EcoT22I 966 ATGCAT ATGCA^T
FbaI 1194 TGATCA T^GATCA
Hin1I 5 GACGCC G[GA]^CG[CT]C
HincII 1403 GTTGAC GT[CT]^[GA]AC
HindII 1403 GTTGAC GT[CT]^[GA]AC
KpnI 615 GGTACC GGTAC^C
MaeIII 524 GTGAC ^GT[ACGT]AC
MluNI 717 TGGCCA TGG^CCA
MscI 717 TGGCCA TGG^CCA
NsiI 966 ATGCAT ATGCA^T
NspI 964 ACATGC [GA]CATG^[CT]
PaeR7I 1223 CTCGAG C^TCGAG
PmaCI 575 CACGTG CAC^GTG
PmlI 575 CACGTG CAC^GTG
PstI 1097 CTGCAG CTGCA^G
PvuII 1115 CAGCTG CAG^CTG
SacI 1560 GAGCTC GAGCT^C
SmaI 53 CCCGGG CCC^GGG
StyI 1285 CCTTGG C^C[AT][AT]GG
TfiI 301 GATTC G^A[AT]TC
Tsp45I 524 GTGAC ^GT[GC]AC
XbaI 856 TCTAGA T^CTAGA
XcmI 1377 CCAAACAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1223 CTCGAG C^TCGAG
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1484 GACATGAAGTC
ApaI 19 GGGCCC GGGCC^C
71 GGGCCC
Asp700I 1007 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAAGCTGTTC
AspEI 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1484 GACATGAAGTC
AspHI 1253 GAGCAC G[AT]GC[AT]^C
1560 GAGCTC
AvaI 53 CCCGGG C^[CT]CG[GA]G
1223 CTCGAG
BsaAI 575 CACGTG [CT]AC^GT[GA]
1198 CACGTA
BsaBI 701 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1189 GATCCTGATC
BsiHKAI 1253 GAGCAC G[AT]GC[AT]^C
1560 GAGCTC
BsoBI 53 CCCGGG C^[CT]CG[GA]G
1223 CTCGAG
BsrI 1456 ACTGG ACTGG(1/-1)
141 CCAGT
DraII 18 GGGGCCC [GA]G^G[ACGT]CC[CT]
474 AGGCCCT
Eam1105I 391 GACAGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1484 GACATGAAGTC
EcoO109I 18 GGGGCCC [GA]G^G[ACGT]CC[CT]
474 AGGCCCT
EcoRV 356 GATATC GAT^ATC
911 GATATC
MamI 701 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1189 GATCCTGATC
MseI 462 TTAA T^TAA
1625 TTAA
PleI 329 GACTC GAGTC(4/5)
1129 GAGTC
SfcI 234 CTATAG C^T[GA][CT]AG
1097 CTGCAG
TaqI 444 TCGA T^CGA
1224 TCGA
Tru9I 462 TTAA T^TAA
1625 TTAA
TthHB8I 444 TCGA T^CGA
1224 TCGA
XmnI 1007 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1234 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TseI Tsp509I TspRI XhoII