##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
ClaI CpoI DraI DraII DrdI
Eam1105I Eco47III EcoO109I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HaeII
Hin1I HincII HindII HpaI KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 289 AACGTT AA^CGTT
AcsI 340 AAATTC [GA]^AATT[CT]
ApoI 340 AAATTC [GA]^AATT[CT]
Asp700I 1253 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 746 GGACC G^G[AT]CC
AviII 94 TGCGCA TGC^GCA
BcnI 179 CCGGG CC^[GC]GG
BlpI 119 GCTTAGC GC^T[ACGT]AGC
BpmI 306 CTGGAG CTGGAG(16/14)
Bpu1102I 119 GCTTAGC GC^T[ACGT]AGC
BsaI 1107 GAGACC GGTCTC(1/5)
BsiEI 172 CGGCCG CG[GA][CT]^CG
BsmI 837 GAATGC GAATGC(1/-1)
BspLU11I 1207 ACATGT A^CATGT
BspMI 91 ACCTGC ACCTGC(4/8)
BsrDI 351 GCAATG GCAATG(2/0)
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BstEII 447 GGTCACC G^GT[ACGT]ACC
BstPI 447 GGTCACC G^GT[ACGT]ACC
CelII 119 GCTTAGC GC^T[ACGT]AGC
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
DraIII 1272 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 137 CCGCGG C^C[GA][CT]GG
EaeI 172 CGGCCG [CT]^GGCC[GA]
EagI 172 CGGCCG C^GGCCG
EclXI 172 CGGCCG C^GGCCG
Eco52I 172 CGGCCG C^GGCCG
EcoO65I 447 GGTCACC G^GT[ACGT]ACC
FauI 139 GCGGG CCCGC(4/6)
FspI 94 TGCGCA TGC^GCA
HgaI 144 GACGC GACGC(5/10)
KspI 137 CCGCGG CCGC^GG
NaeI 24 GCCGGC GCC^GGC
NciI 179 CCGGG CC^[GC]GG
NgoMIV 24 GCCGGC G^CCGGC
NspI 1207 ACATGT [GA]CATG^[CT]
PflMI 959 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 289 AACGTT AA^CGTT
SacII 137 CCGCGG CCGC^GG
SfcI 37 CTGTAG C^T[GA][CT]AG
Van91I 959 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1253 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1207 ACATGT A^C[GA][CT]GT
1227 ACGTGT
AocI 476 CCTAAGG CC^T[ACGT]AGG
749 CCTCAGG
BanII 639 GAGCTC G[GA]GC[CT]^C
678 GAGCTC
BbsI 1066 GAAGAC GAAGAC(2/6)
799 GTCTTC
BfaI 51 CTAG C^TAG
60 CTAG
BpuAI 1066 GAAGAC GAAGAC(2/6)
799 GTCTTC
BseRI 159 GAGGAG GAGGAG(10/8)
300 GAGGAG
BstF5I 296 GGATG GGATG(2/0)
311 GGATG
BstYI 245 GGATCT [GA]^GATC[CT]
778 AGATCC
Bsu36I 476 CCTAAGG CC^T[ACGT]AGG
749 CCTCAGG
Cfr13I 440 GGCCC G^G[ACGT]CC
746 GGACC
EarI 66 GAAGAG CTCTTC(1/4)
279 GAAGAG
Eco81I 476 CCTAAGG CC^T[ACGT]AGG
749 CCTCAGG
EcoNI 542 CCTGATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
733 CCTTAGAAAGG
FokI 296 GGATG GGATG(9/13)
311 GGATG
HindIII 1006 AAGCTT A^AGCTT
1254 AAGCTT
HphI 387 GGTGA GGTGA(8/7)
449 TCACC
Ksp632I 66 GAAGAG CTCTTC(1/4)
279 GAAGAG
MaeI 51 CTAG C^TAG
60 CTAG
MflI 245 GGATCT [GA]^GATC[CT]
778 AGATCC
NlaIV 27 GGCTCC GG[ACGT]^[ACGT]CC
194 GGCTCC
PleI 487 GACTC GAGTC(4/5)
1069 GACTC
PvuII 105 CAGCTG CAG^CTG
960 CAGCTG
SacI 639 GAGCTC GAGCT^C
678 GAGCTC
Sau96I 440 GGCCC G^G[ACGT]CC
746 GGACC
XhoII 245 GGATCT [GA]^GATC[CT]
778 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI BbvI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII RsaI Sau3AI ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I