################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AflII AgeI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BcgI BclI BfrI BlnI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MfeI MluI MunI NdeI NotI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SfiI SgrAI SpeI SphI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AatII 2265 GACGTC GACGT^C AccI 1443 GTCTAC GT^[AC][GT]AC AccII 1305 CGCG CG^CG AccIII 410 TCCGGA T^CCGGA AclI 2217 AACGTT AA^CGTT AcsI 973 GAATTT [GA]^AATT[CT] AhdI 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1768 AGCGCT AGC^GCT ApaI 2056 GGGCCC GGGCC^C ApoI 973 GAATTT [GA]^AATT[CT] Asp700I 1674 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1906 TGGCCA TGG^CCA BbeI 27 GGCGCC GGCGC^C BbsI 126 GAAGAC GAAGAC(2/6) BbvCI 2958 GCTGAGG CCTCAGC(-5/-2) BciVI 1147 GGATAC GTATCC(6/5) BglI 1908 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1930 AGATCT A^GATCT BlpI 2396 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2396 GCTCAGC GC^T[ACGT]AGC BpuAI 126 GAAGAC GAAGAC(2/6) BsaAI 978 TACGTA [CT]AC^GT[GA] BsaBI 414 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 410 TCCGGA [AT]^CCGG[AT] BseAI 410 TCCGGA T^CCGGA BspEI 410 TCCGGA T^CCGGA BssSI 54 CTCGTG CACGAG(-5/-1) BstBI 1353 TTCGAA TT^CGAA BstUI 1305 CGCG CG^CG CelII 2396 GCTCAGC GC^T[ACGT]AGC DraI 2919 TTTAAA TTT^AAA Eam1105I 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1768 AGCGCT AGC^GCT KasI 27 GGCGCC G^GCGCC MamI 414 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1906 TGGCCA TGG^CCA MroI 410 TCCGGA T^CCGGA MscI 1906 TGGCCA TGG^CCA MspA1I 1800 CAGCTG C[AC]G^C[GT]G MvnI 1305 CGCG CG^CG NarI 27 GGCGCC GG^CGCC NheI 2765 GCTAGC G^CTAGC NruI 1304 TCGCGA TCG^CGA NspV 1353 TTCGAA TT^CGAA PaeR7I 874 CTCGAG C^TCGAG Psp1406I 2217 AACGTT AA^CGTT PvuII 1800 CAGCTG CAG^CTG SapI 1674 GAAGAGC GCTCTTC(1/4) SexAI 2002 ACCTGGT A^CC[AT]GGT SfoI 27 GGCGCC GGC^GCC SfuI 1353 TTCGAA TT^CGAA SmaI 41 CCCGGG CCC^GGG SnaBI 978 TACGTA TAC^GTA XhoI 874 CTCGAG C^TCGAG XmaCI 41 CCCGGG C^CCGGG XmaI 41 CCCGGG C^CCGGG XmnI 1674 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 1716 GTGCAC G^TGCAC 2274 GTGCAC AlwNI 880 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 1953 CAGGCCCTG ApaLI 1716 GTGCAC G^TGCAC 2274 GTGCAC BamHI 1401 GGATCC G^GATCC 2992 GGATCC BanI 27 GGCGCC G^G[CT][GA]CC 1347 GGCACC BsaI 1258 GAGACC GGTCTC(1/5) 181 GGTCTC BsmI 2761 GAATGC GAATGC(1/-1) 869 GCATTC BspHI 389 TCATGA T^CATGA 1009 TCATGA BspLU11I 307 ACATGT A^CATGT 2131 ACATGT BspMI 1735 ACCTGC ACCTGC(4/8) 2636 GCAGGT BsrFI 33 GCCGGC [GA]^CCGG[CT] 1966 GCCGGC Cfr10I 33 GCCGGC [GA]^CCGG[CT] 1966 GCCGGC DsaI 2035 CCATGG C^C[GA][CT]GG 2458 CCATGG EaeI 1586 TGGCCG [CT]^GGCC[GA] 1906 TGGCCA FauI 3019 CCCGC CCCGC(4/6) 17 GCGGG HaeII 27 GGCGCC [GA]GCGC^[CT] 1768 AGCGCT NaeI 33 GCCGGC GCC^GGC 1966 GCCGGC NcoI 2035 CCATGG C^CATGG 2458 CCATGG NgoMIV 33 GCCGGC G^CCGGC 1966 GCCGGC NspI 307 ACATGT [GA]CATG^[CT] 2131 ACATGT RcaI 389 TCATGA T^CATGA 1009 TCATGA SacI 2394 GAGCTC GAGCT^C 2586 GAGCTC SbfI 1327 CCTGCAGG CCTGCA^GG 2672 CCTGCAGG ScaI 759 AGTACT AGT^ACT 2098 AGTACT Sse8387I 1327 CCTGCAGG CCTGCA^GG 2672 CCTGCAGG XcmI 1909 CCAACATGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2647 CCATCTTCAGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AocI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII