##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbrPI BcgI BclI BfrI BlnI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MfeI MluI MunI NdeI
NotI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SfiI
SgrAI SpeI SphI SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 2265 GACGTC GACGT^C
AccI 1443 GTCTAC GT^[AC][GT]AC
AccII 1305 CGCG CG^CG
AccIII 410 TCCGGA T^CCGGA
AclI 2217 AACGTT AA^CGTT
AcsI 973 GAATTT [GA]^AATT[CT]
AhdI 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1768 AGCGCT AGC^GCT
ApaI 2056 GGGCCC GGGCC^C
ApoI 973 GAATTT [GA]^AATT[CT]
Asp700I 1674 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1906 TGGCCA TGG^CCA
BbeI 27 GGCGCC GGCGC^C
BbsI 126 GAAGAC GAAGAC(2/6)
BbvCI 2958 GCTGAGG CCTCAGC(-5/-2)
BciVI 1147 GGATAC GTATCC(6/5)
BglI 1908 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1930 AGATCT A^GATCT
BlpI 2396 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2396 GCTCAGC GC^T[ACGT]AGC
BpuAI 126 GAAGAC GAAGAC(2/6)
BsaAI 978 TACGTA [CT]AC^GT[GA]
BsaBI 414 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 410 TCCGGA [AT]^CCGG[AT]
BseAI 410 TCCGGA T^CCGGA
BspEI 410 TCCGGA T^CCGGA
BssSI 54 CTCGTG CACGAG(-5/-1)
BstBI 1353 TTCGAA TT^CGAA
BstUI 1305 CGCG CG^CG
CelII 2396 GCTCAGC GC^T[ACGT]AGC
DraI 2919 TTTAAA TTT^AAA
Eam1105I 249 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1768 AGCGCT AGC^GCT
KasI 27 GGCGCC G^GCGCC
MamI 414 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1906 TGGCCA TGG^CCA
MroI 410 TCCGGA T^CCGGA
MscI 1906 TGGCCA TGG^CCA
MspA1I 1800 CAGCTG C[AC]G^C[GT]G
MvnI 1305 CGCG CG^CG
NarI 27 GGCGCC GG^CGCC
NheI 2765 GCTAGC G^CTAGC
NruI 1304 TCGCGA TCG^CGA
NspV 1353 TTCGAA TT^CGAA
PaeR7I 874 CTCGAG C^TCGAG
Psp1406I 2217 AACGTT AA^CGTT
PvuII 1800 CAGCTG CAG^CTG
SapI 1674 GAAGAGC GCTCTTC(1/4)
SexAI 2002 ACCTGGT A^CC[AT]GGT
SfoI 27 GGCGCC GGC^GCC
SfuI 1353 TTCGAA TT^CGAA
SmaI 41 CCCGGG CCC^GGG
SnaBI 978 TACGTA TAC^GTA
XhoI 874 CTCGAG C^TCGAG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
XmnI 1674 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 1716 GTGCAC G^TGCAC
2274 GTGCAC
AlwNI 880 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1953 CAGGCCCTG
ApaLI 1716 GTGCAC G^TGCAC
2274 GTGCAC
BamHI 1401 GGATCC G^GATCC
2992 GGATCC
BanI 27 GGCGCC G^G[CT][GA]CC
1347 GGCACC
BsaI 1258 GAGACC GGTCTC(1/5)
181 GGTCTC
BsmI 2761 GAATGC GAATGC(1/-1)
869 GCATTC
BspHI 389 TCATGA T^CATGA
1009 TCATGA
BspLU11I 307 ACATGT A^CATGT
2131 ACATGT
BspMI 1735 ACCTGC ACCTGC(4/8)
2636 GCAGGT
BsrFI 33 GCCGGC [GA]^CCGG[CT]
1966 GCCGGC
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
1966 GCCGGC
DsaI 2035 CCATGG C^C[GA][CT]GG
2458 CCATGG
EaeI 1586 TGGCCG [CT]^GGCC[GA]
1906 TGGCCA
FauI 3019 CCCGC CCCGC(4/6)
17 GCGGG
HaeII 27 GGCGCC [GA]GCGC^[CT]
1768 AGCGCT
NaeI 33 GCCGGC GCC^GGC
1966 GCCGGC
NcoI 2035 CCATGG C^CATGG
2458 CCATGG
NgoMIV 33 GCCGGC G^CCGGC
1966 GCCGGC
NspI 307 ACATGT [GA]CATG^[CT]
2131 ACATGT
RcaI 389 TCATGA T^CATGA
1009 TCATGA
SacI 2394 GAGCTC GAGCT^C
2586 GAGCTC
SbfI 1327 CCTGCAGG CCTGCA^GG
2672 CCTGCAGG
ScaI 759 AGTACT AGT^ACT
2098 AGTACT
Sse8387I 1327 CCTGCAGG CCTGCA^GG
2672 CCTGCAGG
XcmI 1909 CCAACATGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2647 CCATCTTCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AocI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII