##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

Acc65I      AflII       AgeI        AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BbrPI       BcgI        BclI        BfrI        BlnI        
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BsrBI       BsrGI       BssHII      Bst1107I    BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        EagI        EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KpnI        
KspI        MfeI        MluI        MunI        NdeI        
NotI        NsiI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SfiI        
SgrAI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AatII        2265  GACGTC               GACGT^C
AccI         1443  GTCTAC               GT^[AC][GT]AC
AccII        1305  CGCG                 CG^CG
AccIII        410  TCCGGA               T^CCGGA
AclI         2217  AACGTT               AA^CGTT
AcsI          973  GAATTT               [GA]^AATT[CT]
AhdI          249  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1768  AGCGCT               AGC^GCT
ApaI         2056  GGGCCC               GGGCC^C
ApoI          973  GAATTT               [GA]^AATT[CT]
Asp700I      1674  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         249  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1906  TGGCCA               TGG^CCA
BbeI           27  GGCGCC               GGCGC^C
BbsI          126  GAAGAC               GAAGAC(2/6)
BbvCI        2958  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1147  GGATAC               GTATCC(6/5)
BglI         1908  GCCAACATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1930  AGATCT               A^GATCT
BlpI         2396  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2396  GCTCAGC              GC^T[ACGT]AGC
BpuAI         126  GAAGAC               GAAGAC(2/6)
BsaAI         978  TACGTA               [CT]AC^GT[GA]
BsaBI         414  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         410  TCCGGA               [AT]^CCGG[AT]
BseAI         410  TCCGGA               T^CCGGA
BspEI         410  TCCGGA               T^CCGGA
BssSI          54  CTCGTG               CACGAG(-5/-1)
BstBI        1353  TTCGAA               TT^CGAA
BstUI        1305  CGCG                 CG^CG
CelII        2396  GCTCAGC              GC^T[ACGT]AGC
DraI         2919  TTTAAA               TTT^AAA
Eam1105I      249  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1768  AGCGCT               AGC^GCT
KasI           27  GGCGCC               G^GCGCC
MamI          414  GATGGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1906  TGGCCA               TGG^CCA
MroI          410  TCCGGA               T^CCGGA
MscI         1906  TGGCCA               TGG^CCA
MspA1I       1800  CAGCTG               C[AC]G^C[GT]G
MvnI         1305  CGCG                 CG^CG
NarI           27  GGCGCC               GG^CGCC
NheI         2765  GCTAGC               G^CTAGC
NruI         1304  TCGCGA               TCG^CGA
NspV         1353  TTCGAA               TT^CGAA
PaeR7I        874  CTCGAG               C^TCGAG
Psp1406I     2217  AACGTT               AA^CGTT
PvuII        1800  CAGCTG               CAG^CTG
SapI         1674  GAAGAGC              GCTCTTC(1/4)
SexAI        2002  ACCTGGT              A^CC[AT]GGT
SfoI           27  GGCGCC               GGC^GCC
SfuI         1353  TTCGAA               TT^CGAA
SmaI           41  CCCGGG               CCC^GGG
SnaBI         978  TACGTA               TAC^GTA
XhoI          874  CTCGAG               C^TCGAG
XmaCI          41  CCCGGG               C^CCGGG
XmaI           41  CCCGGG               C^CCGGG
XmnI         1674  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Alw44I       1716  GTGCAC               G^TGCAC
             2274  GTGCAC              
AlwNI         880  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1953  CAGGCCCTG           
ApaLI        1716  GTGCAC               G^TGCAC
             2274  GTGCAC              
BamHI        1401  GGATCC               G^GATCC
             2992  GGATCC              
BanI           27  GGCGCC               G^G[CT][GA]CC
             1347  GGCACC              
BsaI         1258  GAGACC               GGTCTC(1/5)
              181  GGTCTC              
BsmI         2761  GAATGC               GAATGC(1/-1)
              869  GCATTC              
BspHI         389  TCATGA               T^CATGA
             1009  TCATGA              
BspLU11I      307  ACATGT               A^CATGT
             2131  ACATGT              
BspMI        1735  ACCTGC               ACCTGC(4/8)
             2636  GCAGGT              
BsrFI          33  GCCGGC               [GA]^CCGG[CT]
             1966  GCCGGC              
Cfr10I         33  GCCGGC               [GA]^CCGG[CT]
             1966  GCCGGC              
DsaI         2035  CCATGG               C^C[GA][CT]GG
             2458  CCATGG              
EaeI         1586  TGGCCG               [CT]^GGCC[GA]
             1906  TGGCCA              
FauI         3019  CCCGC                CCCGC(4/6)
               17  GCGGG               
HaeII          27  GGCGCC               [GA]GCGC^[CT]
             1768  AGCGCT              
NaeI           33  GCCGGC               GCC^GGC
             1966  GCCGGC              
NcoI         2035  CCATGG               C^CATGG
             2458  CCATGG              
NgoMIV         33  GCCGGC               G^CCGGC
             1966  GCCGGC              
NspI          307  ACATGT               [GA]CATG^[CT]
             2131  ACATGT              
RcaI          389  TCATGA               T^CATGA
             1009  TCATGA              
SacI         2394  GAGCTC               GAGCT^C
             2586  GAGCTC              
SbfI         1327  CCTGCAGG             CCTGCA^GG
             2672  CCTGCAGG            
ScaI          759  AGTACT               AGT^ACT
             2098  AGTACT              
Sse8387I     1327  CCTGCAGG             CCTGCA^GG
             2672  CCTGCAGG            
XcmI         1909  CCAACATGGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2647  CCATCTTCAGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        AocI        AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII