##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaBI BsiWI BspDI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MluI
NcoI NdeI NheI NruI NsiI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1471 CGCG CG^CG
AccIII 952 TCCGGA T^CCGGA
AcsI 158 GAATTC [GA]^AATT[CT]
AcyI 1617 GGCGCC G[GA]^CG[CT]C
Alw44I 2352 GTGCAC G^TGCAC
ApaLI 2352 GTGCAC G^TGCAC
ApoI 158 GAATTC [GA]^AATT[CT]
AviII 1786 TGCGCA TGC^GCA
BanI 1617 GGCGCC G^G[CT][GA]CC
BbeI 1617 GGCGCC GGCGC^C
BbsI 1544 GAAGAC GAAGAC(2/6)
BglII 1484 AGATCT A^GATCT
BpuAI 1544 GAAGAC GAAGAC(2/6)
BsaHI 1617 GGCGCC G[GA]^CG[CT]C
BsaI 2034 GAGACC GGTCTC(1/5)
BseAI 952 TCCGGA T^CCGGA
BsiEI 1467 CGGCCG CG[GA][CT]^CG
BsmBI 2309 CGTCTC CGTCTC(1/5)
BsmI 67 GCATTC GAATGC(1/-1)
BspEI 952 TCCGGA T^CCGGA
BstUI 1471 CGCG CG^CG
BstXI 1913 CCAGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1467 CGGCCG C^GGCCG
EclXI 1467 CGGCCG C^GGCCG
Eco52I 1467 CGGCCG C^GGCCG
EcoNI 1391 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 158 GAATTC G^AATTC
FspI 1786 TGCGCA TGC^GCA
Hin1I 1617 GGCGCC G[GA]^CG[CT]C
KasI 1617 GGCGCC G^GCGCC
MfeI 298 CAATTG C^AATTG
MroI 952 TCCGGA T^CCGGA
MunI 298 CAATTG C^AATTG
MvnI 1471 CGCG CG^CG
NarI 1617 GGCGCC GG^CGCC
NotI 1466 GCGGCCGC GC^GGCCGC
PaeR7I 2031 CTCGAG C^TCGAG
PpuMI 1069 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1793 GCTCTTC GCTCTTC(1/4)
SfoI 1617 GGCGCC GGC^GCC
XhoI 2031 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 251 GTCTAC GT^[AC][GT]AC
500 GTAGAC
AlwI 636 GATCC GGATC(4/5)
1598 GATCC
AocI 90 CCTTAGG CC^T[ACGT]AGG
2087 CCTCAGG
BalI 1922 TGGCCA TGG^CCA
2405 TGGCCA
BlpI 716 GCTCAGC GC^T[ACGT]AGC
2578 GCTCAGC
Bpu1102I 716 GCTCAGC GC^T[ACGT]AGC
2578 GCTCAGC
BsaWI 952 TCCGGA [AT]^CCGG[AT]
2509 ACCGGA
Bsp1407I 539 TGTACA T^GTACA
2269 TGTACA
BsrGI 539 TGTACA T^GTACA
2269 TGTACA
Bsu36I 90 CCTTAGG CC^T[ACGT]AGG
2087 CCTCAGG
CelII 716 GCTCAGC GC^T[ACGT]AGC
2578 GCTCAGC
EarI 1632 CTCTTC CTCTTC(1/4)
1794 CTCTTC
Eco81I 90 CCTTAGG CC^T[ACGT]AGG
2087 CCTCAGG
EcoT14I 1697 CCAAGG C^C[AT][AT]GG
1919 CCTTGG
HinfI 917 GAATC G^A[ACGT]TC
1652 GAATC
Ksp632I 1632 CTCTTC CTCTTC(1/4)
1794 CTCTTC
MluNI 1922 TGGCCA TGG^CCA
2405 TGGCCA
MscI 1922 TGGCCA TGG^CCA
2405 TGGCCA
MslI 1313 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1374 CACAACCGTG
NaeI 1027 GCCGGC GCC^GGC
1770 GCCGGC
NgoMIV 1027 GCCGGC G^CCGGC
1770 GCCGGC
NspI 1427 GCATGT [GA]CATG^[CT]
1688 GCATGT
SbfI 623 CCTGCAGG CCTGCA^GG
863 CCTGCAGG
Sse8387I 623 CCTGCAGG CCTGCA^GG
863 CCTGCAGG
SspBI 539 TGTACA T^GTACA
2269 TGTACA
StyI 1697 CCAAGG C^C[AT][AT]GG
1919 CCTTGG
TfiI 917 GAATC G^A[AT]TC
1652 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII