##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BbvCI
BcgI BclI BfrI BglII BlnI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco47III Eco81I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MfeI MluI MroI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 758 GTAGAC GT^[AC][GT]AC
ApaI 1021 GGGCCC GGGCC^C
BamHI 2388 GGATCC G^GATCC
BbrPI 352 CACGTG CAC^GTG
BglI 1429 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 745 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 745 GCTCAGC GC^T[ACGT]AGC
BsaWI 262 TCCGGT [AT]^CCGG[AT]
BsiEI 157 CGGCCG CG[GA][CT]^CG
BstEII 319 GGTCACC G^GT[ACGT]ACC
BstPI 319 GGTCACC G^GT[ACGT]ACC
BstXI 1466 CCAGAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2388 GGATCC [GA]^GATC[CT]
CelII 745 GCTCAGC GC^T[ACGT]AGC
EagI 157 CGGCCG C^GGCCG
EclXI 157 CGGCCG C^GGCCG
Eco52I 157 CGGCCG C^GGCCG
EcoO65I 319 GGTCACC G^GT[ACGT]ACC
EcoRV 1667 GATATC GAT^ATC
HincII 1205 GTCAAC GT[CT]^[GA]AC
HindII 1205 GTCAAC GT[CT]^[GA]AC
KspI 154 CCGCGG CCGC^GG
MflI 2388 GGATCC [GA]^GATC[CT]
MseI 1305 TTAA T^TAA
NspI 1366 GCATGT [GA]CATG^[CT]
PmaCI 352 CACGTG CAC^GTG
PmlI 352 CACGTG CAC^GTG
SacII 154 CCGCGG CCGC^GG
SapI 1850 GAAGAGC GCTCTTC(1/4)
SmlI 1439 CTTGAG C^T[CT][GA]AG
TfiI 1809 GAATC G^A[AT]TC
Tru9I 1305 TTAA T^TAA
Tsp509I 1497 AATT ^AATT
XbaI 1279 TCTAGA T^CTAGA
XhoII 2388 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1976 GACAAGTTGTC
AspEI 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1976 GACAAGTTGTC
BalI 510 TGGCCA TGG^CCA
2212 TGGCCA
BbeI 174 GGCGCC GGCGC^C
800 GGCGCC
BciVI 1565 GGATAC GTATCC(6/5)
697 GTATCC
BsaAI 352 CACGTG [CT]AC^GT[GA]
647 TACGTG
BsaI 218 GAGACC GGTCTC(1/5)
1251 GGTCTC
BsmBI 2107 CGTCTC CGTCTC(1/5)
930 GAGACG
BsmI 463 GAATGC GAATGC(1/-1)
2338 GAATGC
Bsp1407I 856 TGTACA T^GTACA
1194 TGTACA
BsrBI 83 CCGCTC CCGCTC(-3/-3)
2071 CCGCTC
BsrDI 1897 CATTGC GCAATG(2/0)
2477 GCAATG
BsrGI 856 TGTACA T^GTACA
1194 TGTACA
Eam1105I 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1976 GACAAGTTGTC
KasI 174 GGCGCC G^GCGCC
800 GGCGCC
MaeII 353 ACGT A^CGT
648 ACGT
MluNI 510 TGGCCA TGG^CCA
2212 TGGCCA
MscI 510 TGGCCA TGG^CCA
2212 TGGCCA
NaeI 159 GCCGGC GCC^GGC
726 GCCGGC
NarI 174 GGCGCC GG^CGCC
800 GGCGCC
NcoI 44 CCATGG C^CATGG
2413 CCATGG
NgoMIV 159 GCCGGC G^CCGGC
726 GCCGGC
PflMI 920 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2044 CCAGCGTATGG
SacI 743 GAGCTC GAGCT^C
1461 GAGCTC
SfoI 174 GGCGCC GGC^GCC
800 GGCGCC
SmaI 169 CCCGGG CCC^GGG
687 CCCGGG
SspBI 856 TGTACA T^GTACA
1194 TGTACA
StuI 411 AGGCCT AGG^CCT
1525 AGGCCT
Van91I 920 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2044 CCAGCGTATGG
XcmI 2116 CCAGTTCAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2439 CCAGCCCCATTATGG
XmaCI 169 CCCGGG C^CCGGG
687 CCCGGG
XmaI 169 CCCGGG C^CCGGG
687 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI