################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BbvCI BcgI BclI BfrI BglII BlnI BsaBI BseAI BsiWI BspDI BspEI BspHI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI MamI MfeI MluI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 758 GTAGAC GT^[AC][GT]AC ApaI 1021 GGGCCC GGGCC^C BamHI 2388 GGATCC G^GATCC BbrPI 352 CACGTG CAC^GTG BglI 1429 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 745 GCTCAGC GC^T[ACGT]AGC Bpu1102I 745 GCTCAGC GC^T[ACGT]AGC BsaWI 262 TCCGGT [AT]^CCGG[AT] BsiEI 157 CGGCCG CG[GA][CT]^CG BstEII 319 GGTCACC G^GT[ACGT]ACC BstPI 319 GGTCACC G^GT[ACGT]ACC BstXI 1466 CCAGAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2388 GGATCC [GA]^GATC[CT] CelII 745 GCTCAGC GC^T[ACGT]AGC EagI 157 CGGCCG C^GGCCG EclXI 157 CGGCCG C^GGCCG Eco52I 157 CGGCCG C^GGCCG EcoO65I 319 GGTCACC G^GT[ACGT]ACC EcoRV 1667 GATATC GAT^ATC HincII 1205 GTCAAC GT[CT]^[GA]AC HindII 1205 GTCAAC GT[CT]^[GA]AC KspI 154 CCGCGG CCGC^GG MflI 2388 GGATCC [GA]^GATC[CT] MseI 1305 TTAA T^TAA NspI 1366 GCATGT [GA]CATG^[CT] PmaCI 352 CACGTG CAC^GTG PmlI 352 CACGTG CAC^GTG SacII 154 CCGCGG CCGC^GG SapI 1850 GAAGAGC GCTCTTC(1/4) SmlI 1439 CTTGAG C^T[CT][GA]AG TfiI 1809 GAATC G^A[AT]TC Tru9I 1305 TTAA T^TAA Tsp509I 1497 AATT ^AATT XbaI 1279 TCTAGA T^CTAGA XhoII 2388 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AhdI 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1976 GACAAGTTGTC AspEI 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1976 GACAAGTTGTC BalI 510 TGGCCA TGG^CCA 2212 TGGCCA BbeI 174 GGCGCC GGCGC^C 800 GGCGCC BciVI 1565 GGATAC GTATCC(6/5) 697 GTATCC BsaAI 352 CACGTG [CT]AC^GT[GA] 647 TACGTG BsaI 218 GAGACC GGTCTC(1/5) 1251 GGTCTC BsmBI 2107 CGTCTC CGTCTC(1/5) 930 GAGACG BsmI 463 GAATGC GAATGC(1/-1) 2338 GAATGC Bsp1407I 856 TGTACA T^GTACA 1194 TGTACA BsrBI 83 CCGCTC CCGCTC(-3/-3) 2071 CCGCTC BsrDI 1897 CATTGC GCAATG(2/0) 2477 GCAATG BsrGI 856 TGTACA T^GTACA 1194 TGTACA Eam1105I 1244 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1976 GACAAGTTGTC KasI 174 GGCGCC G^GCGCC 800 GGCGCC MaeII 353 ACGT A^CGT 648 ACGT MluNI 510 TGGCCA TGG^CCA 2212 TGGCCA MscI 510 TGGCCA TGG^CCA 2212 TGGCCA NaeI 159 GCCGGC GCC^GGC 726 GCCGGC NarI 174 GGCGCC GG^CGCC 800 GGCGCC NcoI 44 CCATGG C^CATGG 2413 CCATGG NgoMIV 159 GCCGGC G^CCGGC 726 GCCGGC PflMI 920 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2044 CCAGCGTATGG SacI 743 GAGCTC GAGCT^C 1461 GAGCTC SfoI 174 GGCGCC GGC^GCC 800 GGCGCC SmaI 169 CCCGGG CCC^GGG 687 CCCGGG SspBI 856 TGTACA T^GTACA 1194 TGTACA StuI 411 AGGCCT AGG^CCT 1525 AGGCCT Van91I 920 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2044 CCAGCGTATGG XcmI 2116 CCAGTTCAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2439 CCAGCCCCATTATGG XmaCI 169 CCCGGG C^CCGGG 687 CCCGGG XmaI 169 CCCGGG C^CCGGG 687 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI AlwI AlwNI Asp718I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI