################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AclI AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AviII AvrII BalI BbrPI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HincII HindII HpaI KspI MluI MluNI MscI NaeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SnaBI SpeI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 1595 TCCGGA T^CCGGA AspI 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 1331 GTATCC GTATCC(6/5) BclI 442 TGATCA T^GATCA BglII 2544 AGATCT A^GATCT BsaBI 2668 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1595 TCCGGA T^CCGGA BspEI 1595 TCCGGA T^CCGGA BssHII 199 GCGCGC G^CGCGC BstBI 2240 TTCGAA TT^CGAA BstEII 351 GGTGACC G^GT[ACGT]ACC BstPI 351 GGTGACC G^GT[ACGT]ACC DraIII 1484 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1632 TGGCCG [CT]^GGCC[GA] EcoO65I 351 GGTGACC G^GT[ACGT]ACC FbaI 442 TGATCA T^GATCA HindIII 2615 AAGCTT A^AGCTT MamI 2668 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2160 CAATTG C^AATTG MroI 1595 TCCGGA T^CCGGA MunI 2160 CAATTG C^AATTG NspV 2240 TTCGAA TT^CGAA PflFI 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1801 GGGACCT [GA]G^G[AT]CC[CT] PvuII 298 CAGCTG CAG^CTG SbfI 3053 CCTGCAGG CCTGCA^GG SfuI 2240 TTCGAA TT^CGAA SgrAI 1926 CACCGGTG C[GA]^CCGG[CT]G SmlI 374 CTCAAG C^T[CT][GA]AG Sse8387I 3053 CCTGCAGG CCTGCA^GG Tth111I 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2518 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 2064 GGTACC G^GTACC 2249 GGTACC AcyI 46 GGCGCC G[GA]^CG[CT]C 111 GGCGCC AgeI 1437 ACCGGT A^CCGGT 1927 ACCGGT ApaI 326 GGGCCC GGGCC^C 2984 GGGCCC Asp718I 2064 GGTACC G^GTACC 2249 GGTACC BamHI 1039 GGATCC G^GATCC 2933 GGATCC BbeI 46 GGCGCC GGCGC^C 111 GGCGCC BbvCI 602 CCTCAGC CCTCAGC(-5/-2) 2488 CCTCAGC BsaHI 46 GGCGCC G[GA]^CG[CT]C 111 GGCGCC BsgI 91 CTGCAC GTGCAG(16/14) 1013 CTGCAC BsmBI 944 CGTCTC CGTCTC(1/5) 1591 CGTCTC BspMI 1761 ACCTGC ACCTGC(4/8) 2273 ACCTGC BstAPI 1245 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1687 GCATCCCCTGC EcoNI 1805 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2882 CCTTCCAGAGG EcoRI 932 GAATTC G^AATTC 1740 GAATTC HgaI 1684 GACGC GACGC(5/10) 2448 GCGTC Hin1I 46 GGCGCC G[GA]^CG[CT]C 111 GGCGCC KasI 46 GGCGCC G^GCGCC 111 GGCGCC KpnI 2064 GGTACC GGTAC^C 2249 GGTACC MaeII 1017 ACGT A^CGT 1505 ACGT MspA1I 298 CAGCTG C[AC]G^C[GT]G 1978 CCGCTG NarI 46 GGCGCC GG^CGCC 111 GGCGCC NdeI 1582 CATATG CA^TATG 2177 CATATG PflMI 1024 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2391 CCAATCTCTGG PinAI 1437 ACCGGT A^CCGGT 1927 ACCGGT PstI 810 CTGCAG CTGCA^G 3054 CTGCAG SacI 1049 GAGCTC GAGCT^C 3151 GAGCTC SfoI 46 GGCGCC GGC^GCC 111 GGCGCC SphI 1204 GCATGC GCATG^C 1399 GCATGC StuI 744 AGGCCT AGG^CCT 1285 AGGCCT TaqI 1529 TCGA T^CGA 2241 TCGA TthHB8I 1529 TCGA T^CGA 2241 TCGA Van91I 1024 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2391 CCAATCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI XmnI