##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AflII AflIII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AviII AvrII BalI
BbrPI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KspI MluI MluNI
MscI NaeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SnaBI SpeI SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1595 TCCGGA T^CCGGA
AspI 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1331 GTATCC GTATCC(6/5)
BclI 442 TGATCA T^GATCA
BglII 2544 AGATCT A^GATCT
BsaBI 2668 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1595 TCCGGA T^CCGGA
BspEI 1595 TCCGGA T^CCGGA
BssHII 199 GCGCGC G^CGCGC
BstBI 2240 TTCGAA TT^CGAA
BstEII 351 GGTGACC G^GT[ACGT]ACC
BstPI 351 GGTGACC G^GT[ACGT]ACC
DraIII 1484 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1632 TGGCCG [CT]^GGCC[GA]
EcoO65I 351 GGTGACC G^GT[ACGT]ACC
FbaI 442 TGATCA T^GATCA
HindIII 2615 AAGCTT A^AGCTT
MamI 2668 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2160 CAATTG C^AATTG
MroI 1595 TCCGGA T^CCGGA
MunI 2160 CAATTG C^AATTG
NspV 2240 TTCGAA TT^CGAA
PflFI 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1801 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 298 CAGCTG CAG^CTG
SbfI 3053 CCTGCAGG CCTGCA^GG
SfuI 2240 TTCGAA TT^CGAA
SgrAI 1926 CACCGGTG C[GA]^CCGG[CT]G
SmlI 374 CTCAAG C^T[CT][GA]AG
Sse8387I 3053 CCTGCAGG CCTGCA^GG
Tth111I 2105 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2518 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 2064 GGTACC G^GTACC
2249 GGTACC
AcyI 46 GGCGCC G[GA]^CG[CT]C
111 GGCGCC
AgeI 1437 ACCGGT A^CCGGT
1927 ACCGGT
ApaI 326 GGGCCC GGGCC^C
2984 GGGCCC
Asp718I 2064 GGTACC G^GTACC
2249 GGTACC
BamHI 1039 GGATCC G^GATCC
2933 GGATCC
BbeI 46 GGCGCC GGCGC^C
111 GGCGCC
BbvCI 602 CCTCAGC CCTCAGC(-5/-2)
2488 CCTCAGC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
111 GGCGCC
BsgI 91 CTGCAC GTGCAG(16/14)
1013 CTGCAC
BsmBI 944 CGTCTC CGTCTC(1/5)
1591 CGTCTC
BspMI 1761 ACCTGC ACCTGC(4/8)
2273 ACCTGC
BstAPI 1245 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1687 GCATCCCCTGC
EcoNI 1805 CCTACACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2882 CCTTCCAGAGG
EcoRI 932 GAATTC G^AATTC
1740 GAATTC
HgaI 1684 GACGC GACGC(5/10)
2448 GCGTC
Hin1I 46 GGCGCC G[GA]^CG[CT]C
111 GGCGCC
KasI 46 GGCGCC G^GCGCC
111 GGCGCC
KpnI 2064 GGTACC GGTAC^C
2249 GGTACC
MaeII 1017 ACGT A^CGT
1505 ACGT
MspA1I 298 CAGCTG C[AC]G^C[GT]G
1978 CCGCTG
NarI 46 GGCGCC GG^CGCC
111 GGCGCC
NdeI 1582 CATATG CA^TATG
2177 CATATG
PflMI 1024 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2391 CCAATCTCTGG
PinAI 1437 ACCGGT A^CCGGT
1927 ACCGGT
PstI 810 CTGCAG CTGCA^G
3054 CTGCAG
SacI 1049 GAGCTC GAGCT^C
3151 GAGCTC
SfoI 46 GGCGCC GGC^GCC
111 GGCGCC
SphI 1204 GCATGC GCATG^C
1399 GCATGC
StuI 744 AGGCCT AGG^CCT
1285 AGGCCT
TaqI 1529 TCGA T^CGA
2241 TCGA
TthHB8I 1529 TCGA T^CGA
2241 TCGA
Van91I 1024 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2391 CCAATCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmaCI XmaI
XmnI