##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BcgI
BfrI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BsrBI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 3158 GGTACC G^GTACC
AccI 3041 GTCTAC GT^[AC][GT]AC
ApaI 2246 GGGCCC GGGCC^C
Asp718I 3158 GGTACC G^GTACC
AvrII 2250 CCTAGG C^CTAGG
BamHI 2585 GGATCC G^GATCC
BbrPI 817 CACGTG CAC^GTG
BciVI 1855 GTATCC GTATCC(6/5)
BglI 1558 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2250 CCTAGG C^CTAGG
BsgI 632 GTGCAG GTGCAG(16/14)
BsmBI 2259 CGTCTC CGTCTC(1/5)
Bsp1407I 1909 TGTACA T^GTACA
BsrGI 1909 TGTACA T^GTACA
BssSI 1905 CTCGTG CACGAG(-5/-1)
BstXI 2336 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1738 CCATGG C^C[GA][CT]GG
EcoNI 1619 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 320 CCCGC CCCGC(4/6)
HgaI 42 GACGC GACGC(5/10)
KpnI 3158 GGTACC GGTAC^C
NcoI 1738 CCATGG C^CATGG
PleI 2223 GACTC GAGTC(4/5)
PmaCI 817 CACGTG CAC^GTG
PmlI 817 CACGTG CAC^GTG
PshAI 42 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2508 GAGCTC GAGCT^C
SapI 756 GAAGAGC GCTCTTC(1/4)
ScaI 3243 AGTACT AGT^ACT
SexAI 343 ACCTGGT A^CC[AT]GGT
SpeI 2388 ACTAGT A^CTAGT
SphI 1400 GCATGC GCATG^C
SspBI 1909 TGTACA T^GTACA
StuI 1914 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 765 CCTGAGG CC^T[ACGT]AGG
1623 CCTAAGG
AvaI 1328 CCCGAG C^[CT]CG[GA]G
1587 CTCGGG
BanII 2246 GGGCCC G[GA]GC[CT]^C
2508 GAGCTC
BbsI 959 GTCTTC GAAGAC(2/6)
1700 GTCTTC
BbvCI 32 CCTCAGC CCTCAGC(-5/-2)
193 CCTCAGC
BclI 864 TGATCA T^GATCA
1838 TGATCA
BcnI 909 CCGGG CC^[GC]GG
2097 CCCGG
BglII 2126 AGATCT A^GATCT
2691 AGATCT
BpuAI 959 GTCTTC GAAGAC(2/6)
1700 GTCTTC
BsoBI 1328 CCCGAG C^[CT]CG[GA]G
1587 CTCGGG
BspMI 1537 GCAGGT ACCTGC(4/8)
1687 GCAGGT
BstEII 1690 GGTAACC G^GT[ACGT]ACC
1947 GGTGACC
BstPI 1690 GGTAACC G^GT[ACGT]ACC
1947 GGTGACC
Bsu36I 765 CCTGAGG CC^T[ACGT]AGG
1623 CCTAAGG
DraI 1981 TTTAAA TTT^AAA
2887 TTTAAA
Eco81I 765 CCTGAGG CC^T[ACGT]AGG
1623 CCTAAGG
EcoO65I 1690 GGTAACC G^GT[ACGT]ACC
1947 GGTGACC
FbaI 864 TGATCA T^GATCA
1838 TGATCA
NciI 909 CCGGG CC^[GC]GG
2097 CCCGG
SfcI 926 CTATAG C^T[GA][CT]AG
2122 CTACAG
SmlI 201 CTTGAG C^T[CT][GA]AG
2267 CTCAAG
TaqI 487 TCGA T^CGA
1223 TCGA
Tsp509I 1439 AATT ^AATT
1956 AATT
TthHB8I 487 TCGA T^CGA
1223 TCGA
XcmI 1002 CCAGGTGAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1760 CCATCTTTACTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaII BalI
BanI BbeI BbvI BfaI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NdeII
NlaIII NlaIV NspI PflMI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI StyI TfiI Tru9I
TseI Tsp45I TspRI Van91I XhoII