################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII BcgI BfrI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 3158 GGTACC G^GTACC AccI 3041 GTCTAC GT^[AC][GT]AC ApaI 2246 GGGCCC GGGCC^C Asp718I 3158 GGTACC G^GTACC AvrII 2250 CCTAGG C^CTAGG BamHI 2585 GGATCC G^GATCC BbrPI 817 CACGTG CAC^GTG BciVI 1855 GTATCC GTATCC(6/5) BglI 1558 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2250 CCTAGG C^CTAGG BsgI 632 GTGCAG GTGCAG(16/14) BsmBI 2259 CGTCTC CGTCTC(1/5) Bsp1407I 1909 TGTACA T^GTACA BsrGI 1909 TGTACA T^GTACA BssSI 1905 CTCGTG CACGAG(-5/-1) BstXI 2336 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 1738 CCATGG C^C[GA][CT]GG EcoNI 1619 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 320 CCCGC CCCGC(4/6) HgaI 42 GACGC GACGC(5/10) KpnI 3158 GGTACC GGTAC^C NcoI 1738 CCATGG C^CATGG PleI 2223 GACTC GAGTC(4/5) PmaCI 817 CACGTG CAC^GTG PmlI 817 CACGTG CAC^GTG PshAI 42 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2508 GAGCTC GAGCT^C SapI 756 GAAGAGC GCTCTTC(1/4) ScaI 3243 AGTACT AGT^ACT SexAI 343 ACCTGGT A^CC[AT]GGT SpeI 2388 ACTAGT A^CTAGT SphI 1400 GCATGC GCATG^C SspBI 1909 TGTACA T^GTACA StuI 1914 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 765 CCTGAGG CC^T[ACGT]AGG 1623 CCTAAGG AvaI 1328 CCCGAG C^[CT]CG[GA]G 1587 CTCGGG BanII 2246 GGGCCC G[GA]GC[CT]^C 2508 GAGCTC BbsI 959 GTCTTC GAAGAC(2/6) 1700 GTCTTC BbvCI 32 CCTCAGC CCTCAGC(-5/-2) 193 CCTCAGC BclI 864 TGATCA T^GATCA 1838 TGATCA BcnI 909 CCGGG CC^[GC]GG 2097 CCCGG BglII 2126 AGATCT A^GATCT 2691 AGATCT BpuAI 959 GTCTTC GAAGAC(2/6) 1700 GTCTTC BsoBI 1328 CCCGAG C^[CT]CG[GA]G 1587 CTCGGG BspMI 1537 GCAGGT ACCTGC(4/8) 1687 GCAGGT BstEII 1690 GGTAACC G^GT[ACGT]ACC 1947 GGTGACC BstPI 1690 GGTAACC G^GT[ACGT]ACC 1947 GGTGACC Bsu36I 765 CCTGAGG CC^T[ACGT]AGG 1623 CCTAAGG DraI 1981 TTTAAA TTT^AAA 2887 TTTAAA Eco81I 765 CCTGAGG CC^T[ACGT]AGG 1623 CCTAAGG EcoO65I 1690 GGTAACC G^GT[ACGT]ACC 1947 GGTGACC FbaI 864 TGATCA T^GATCA 1838 TGATCA NciI 909 CCGGG CC^[GC]GG 2097 CCCGG SfcI 926 CTATAG C^T[GA][CT]AG 2122 CTACAG SmlI 201 CTTGAG C^T[CT][GA]AG 2267 CTCAAG TaqI 487 TCGA T^CGA 1223 TCGA Tsp509I 1439 AATT ^AATT 1956 AATT TthHB8I 487 TCGA T^CGA 1223 TCGA XcmI 1002 CCAGGTGAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1760 CCATCTTTACTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaII BalI BanI BbeI BbvI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NarI NdeII NlaIII NlaIV NspI PflMI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XhoII