##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccIII AclI AcsI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BglI BglII BlnI BlpI
Bpu1102I BsaI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI PvuII RsrII
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 44 GACGTC GACGT^C
Acc65I 1424 GGTACC G^GTACC
AccI 53 GTCTAC GT^[AC][GT]AC
AcyI 44 GACGTC G[GA]^CG[CT]C
AflII 2257 CTTAAG C^TTAAG
AlwNI 1877 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 531 CCTCAGG CC^T[ACGT]AGG
AseI 1580 ATTAAT AT^TAAT
AsnI 1580 ATTAAT AT^TAAT
Asp700I 141 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1424 GGTACC G^GTACC
BamHI 282 GGATCC G^GATCC
BbsI 179 GAAGAC GAAGAC(2/6)
BciVI 226 GTATCC GTATCC(6/5)
BclI 276 TGATCA T^GATCA
BfrI 2257 CTTAAG C^TTAAG
BpuAI 179 GAAGAC GAAGAC(2/6)
BsaAI 1128 TACGTA [CT]AC^GT[GA]
BsaBI 277 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 44 GACGTC G[GA]^CG[CT]C
BsaWI 57 ACCGGA [AT]^CCGG[AT]
BsgI 1451 GTGCAG GTGCAG(16/14)
BsmI 959 GCATTC GAATGC(1/-1)
BspHI 1161 TCATGA T^CATGA
BspMI 1999 ACCTGC ACCTGC(4/8)
BstAPI 1344 GCATCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 282 GGATCC [GA]^GATC[CT]
Bsu36I 531 CCTCAGG CC^T[ACGT]AGG
DraI 2368 TTTAAA TTT^AAA
DraIII 1593 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 44 GACGTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 531 CCTCAGG CC^T[ACGT]AGG
FauI 70 GCGGG CCCGC(4/6)
FbaI 276 TGATCA T^GATCA
HgaI 51 GCGTC GACGC(5/10)
Hin1I 44 GACGTC G[GA]^CG[CT]C
KpnI 1424 GGTACC GGTAC^C
MamI 277 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 282 GGATCC [GA]^GATC[CT]
MslI 1524 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 869 CTCGAG C^TCGAG
PpuMI 2030 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1580 ATTAAT AT^TAAT
RcaI 1161 TCATGA T^CATGA
SapI 1911 GAAGAGC GCTCTTC(1/4)
SmaI 525 CCCGGG CCC^GGG
SnaBI 1128 TACGTA TAC^GTA
SpeI 2197 ACTAGT A^CTAGT
StuI 1313 AGGCCT AGG^CCT
XhoI 869 CTCGAG C^TCGAG
XhoII 282 GGATCC [GA]^GATC[CT]
XmaCI 525 CCCGGG C^CCGGG
XmaI 525 CCCGGG C^CCGGG
XmnI 141 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 197 GAGCTC G[AT]GC[AT]^C
1914 GAGCTC
AvaI 525 CCCGGG C^[CT]CG[GA]G
869 CTCGAG
BseRI 601 CTCCTC GAGGAG(10/8)
2107 CTCCTC
BsiHKAI 197 GAGCTC G[AT]GC[AT]^C
1914 GAGCTC
BsoBI 525 CCCGGG C^[CT]CG[GA]G
869 CTCGAG
BsrDI 2101 CATTGC GCAATG(2/0)
741 GCAATG
EcoNI 1316 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1799 CCTGTGACAGG
EcoRV 674 GATATC GAT^ATC
1843 GATATC
EcoT14I 655 CCATGG C^C[AT][AT]GG
2143 CCATGG
KspI 68 CCGCGG CCGC^GG
82 CCGCGG
MaeII 45 ACGT A^CGT
1129 ACGT
MspA1I 68 CCGCGG C[AC]G^C[GT]G
82 CCGCGG
NcoI 655 CCATGG C^CATGG
2143 CCATGG
SacI 197 GAGCTC GAGCT^C
1914 GAGCTC
SacII 68 CCGCGG CCGC^GG
82 CCGCGG
SmlI 869 CTCGAG C^T[CT][GA]AG
2257 CTTAAG
SspI 491 AATATT AAT^ATT
1472 AATATT
StyI 655 CCATGG C^C[AT][AT]GG
2143 CCATGG
TaqI 348 TCGA T^CGA
870 TCGA
TthHB8I 348 TCGA T^CGA
870 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI