##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII BbeI BbrPI
BbvCI BclI BglI BglII BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoNI FauI FbaI FseI FspI
HaeII HgaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2009 GACGTC GACGT^C
AccI 1171 GTCTAC GT^[AC][GT]AC
AccII 345 CGCG CG^CG
AcyI 2009 GACGTC G[GA]^CG[CT]C
AflII 2640 CTTAAG C^TTAAG
AhdI 2665 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 168 GTGCAC G^TGCAC
AocI 283 CCTGAGG CC^T[ACGT]AGG
ApaLI 168 GTGCAC G^TGCAC
AspEI 2665 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2797 CCTAGG C^CTAGG
BamHI 50 GGATCC G^GATCC
BanI 2378 GGCACC G^G[CT][GA]CC
BbsI 2425 GTCTTC GAAGAC(2/6)
BcgI 1421 CGAACTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1540 GGATAC GTATCC(6/5)
BcnI 2604 CCCGG CC^[GC]GG
BfrI 2640 CTTAAG C^TTAAG
BlnI 2797 CCTAGG C^CTAGG
BpuAI 2425 GTCTTC GAAGAC(2/6)
BsaHI 2009 GACGTC G[GA]^CG[CT]C
BsmBI 2431 CGTCTC CGTCTC(1/5)
BsmI 3562 GAATGC GAATGC(1/-1)
Bsp1407I 809 TGTACA T^GTACA
BspLU11I 618 ACATGT A^CATGT
BspMI 1902 ACCTGC ACCTGC(4/8)
BsrGI 809 TGTACA T^GTACA
BssSI 1286 CACGAG CACGAG(-5/-1)
BstUI 345 CGCG CG^CG
BstYI 50 GGATCC [GA]^GATC[CT]
Bsu36I 283 CCTGAGG CC^T[ACGT]AGG
CfoI 1925 GCGC GCG^C
Eam1105I 2665 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 283 CCTGAGG CC^T[ACGT]AGG
EcoRI 1060 GAATTC G^AATTC
EcoRV 3499 GATATC GAT^ATC
HhaI 1925 GCGC GCG^C
Hin1I 2009 GACGTC G[GA]^CG[CT]C
HinP1I 1925 GCGC G^CGC
HpaI 605 GTTAAC GTT^AAC
MflI 50 GGATCC [GA]^GATC[CT]
MvnI 345 CGCG CG^CG
NciI 2604 CCCGG CC^[GC]GG
PshAI 635 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2126 GAAGAGC GCTCTTC(1/4)
SspBI 809 TGTACA T^GTACA
SspI 1590 AATATT AAT^ATT
XhoII 50 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 618 ACATGT A^C[GA][CT]GT
2421 ACACGT
BalI 835 TGGCCA TGG^CCA
2190 TGGCCA
BanII 2861 GAGCTC G[GA]GC[CT]^C
3057 GAGCTC
BlpI 1247 GCTGAGC GC^T[ACGT]AGC
1824 GCTGAGC
Bpu1102I 1247 GCTGAGC GC^T[ACGT]AGC
1824 GCTGAGC
BseRI 1952 CTCCTC GAGGAG(10/8)
2284 CTCCTC
BspHI 994 TCATGA T^CATGA
1377 TCATGA
BsrDI 228 GCAATG GCAATG(2/0)
3147 GCAATG
BstEII 20 GGTTACC G^GT[ACGT]ACC
3004 GGTAACC
BstPI 20 GGTTACC G^GT[ACGT]ACC
3004 GGTAACC
CelII 1247 GCTGAGC GC^T[ACGT]AGC
1824 GCTGAGC
DraI 3520 TTTAAA TTT^AAA
3617 TTTAAA
DrdI 215 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2900 GACATGCCTGTC
EarI 2126 GAAGAG CTCTTC(1/4)
2895 GAAGAG
EcoO65I 20 GGTTACC G^GT[ACGT]ACC
3004 GGTAACC
HapII 923 CCGG C^CGG
2605 CCGG
HindIII 2203 AAGCTT A^AGCTT
2358 AAGCTT
HpaII 923 CCGG C^CGG
2605 CCGG
Ksp632I 2126 GAAGAG CTCTTC(1/4)
2895 GAAGAG
MaeII 2010 ACGT A^CGT
2423 ACGT
MluNI 835 TGGCCA TGG^CCA
2190 TGGCCA
MscI 835 TGGCCA TGG^CCA
2190 TGGCCA
MspI 923 CCGG C^CGG
2605 CCGG
NcoI 742 CCATGG C^CATGG
2962 CCATGG
NdeI 9 CATATG CA^TATG
3567 CATATG
RcaI 994 TCATGA T^CATGA
1377 TCATGA
SacI 2861 GAGCTC GAGCT^C
3057 GAGCTC
XbaI 1126 TCTAGA T^CTAGA
3137 TCTAGA
XcmI 45 CCATTGGATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
742 CCATGGACTCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI