##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII BbrPI BciVI BclI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FbaI FseI FspI KpnI
KspI MamI MfeI MroI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 3377 GGCGCC G[GA]^CG[CT]C
Alw44I 1568 GTGCAC G^TGCAC
ApaLI 1568 GTGCAC G^TGCAC
BbeI 3377 GGCGCC GGCGC^C
BbvCI 902 CCTCAGC CCTCAGC(-5/-2)
BcgI 325 CGACTTATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 73 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 177 AGATCT A^GATCT
BsaHI 3377 GGCGCC G[GA]^CG[CT]C
BsmBI 321 GAGACG CGTCTC(1/5)
BsrFI 5 GCCGGC [GA]^CCGG[CT]
BssSI 2219 CTCGTG CACGAG(-5/-1)
BstEII 3075 GGTTACC G^GT[ACGT]ACC
BstPI 3075 GGTTACC G^GT[ACGT]ACC
BstXI 2251 CCAACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
EcoNI 1093 CCTCTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3075 GGTTACC G^GT[ACGT]ACC
EcoRI 3783 GAATTC G^AATTC
EcoRV 3809 GATATC GAT^ATC
HgaI 1993 GCGTC GACGC(5/10)
Hin1I 3377 GGCGCC G[GA]^CG[CT]C
HpaI 2237 GTTAAC GTT^AAC
KasI 3377 GGCGCC G^GCGCC
NaeI 5 GCCGGC GCC^GGC
NarI 3377 GGCGCC GG^CGCC
NcoI 3530 CCATGG C^CATGG
NdeI 2603 CATATG CA^TATG
NgoMIV 5 GCCGGC G^CCGGC
SfoI 3377 GGCGCC GGC^GCC
SphI 1067 GCATGC GCATG^C
SspI 3190 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 653 CTTAAG C^TTAAG
1450 CTTAAG
AflIII 703 ACGCGT A^C[GA][CT]GT
1991 ACGCGT
AlwNI 448 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
3393 CAGTACCTG
AvaI 74 CCCGGG C^[CT]CG[GA]G
3373 CCCGGG
AvrII 982 CCTAGG C^CTAGG
1890 CCTAGG
BamHI 2361 GGATCC G^GATCC
2892 GGATCC
BanI 421 GGTGCC G^G[CT][GA]CC
3377 GGCGCC
BanII 71 GAGCCC G[GA]GC[CT]^C
3401 GAGCCC
BfrI 653 CTTAAG C^TTAAG
1450 CTTAAG
BlnI 982 CCTAGG C^CTAGG
1890 CCTAGG
BsaI 3822 GAGACC GGTCTC(1/5)
1386 GGTCTC
BsgI 3489 CTGCAC GTGCAG(16/14)
2222 GTGCAG
BsoBI 74 CCCGGG C^[CT]CG[GA]G
3373 CCCGGG
BspHI 1798 TCATGA T^CATGA
2614 TCATGA
BstAPI 1422 GCAAGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3512 GCAAGATGTGC
EarI 1641 CTCTTC CTCTTC(1/4)
399 GAAGAG
FauI 67 GCGGG CCCGC(4/6)
86 GCGGG
HaeII 2851 GGCGCT [GA]GCGC^[CT]
3377 GGCGCC
Ksp632I 1641 CTCTTC CTCTTC(1/4)
399 GAAGAG
MluI 703 ACGCGT A^CGCGT
1991 ACGCGT
RcaI 1798 TCATGA T^CATGA
2614 TCATGA
SmaI 74 CCCGGG CCC^GGG
3373 CCCGGG
XbaI 3026 TCTAGA T^CTAGA
3605 TCTAGA
XmaCI 74 CCCGGG C^CCGGG
3373 CCCGGG
XmaI 74 CCCGGG C^CCGGG
3373 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BalI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI