##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BamHI BbsI BbvCI BcgI BciVI
BclI BfrI BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MluI MroI
MseI MvnI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tru9I Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Alw44I 445 GTGCAC G^TGCAC
AlwI 1492 GGATC GGATC(4/5)
Aor51HI 1595 AGCGCT AGC^GCT
ApaLI 445 GTGCAC G^TGCAC
AvrII 345 CCTAGG C^CTAGG
BbeI 14 GGCGCC GGCGC^C
BbrPI 1278 CACGTG CAC^GTG
BglII 1288 AGATCT A^GATCT
BlnI 345 CCTAGG C^CTAGG
BlpI 1777 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1777 GCTGAGC GC^T[ACGT]AGC
BsaAI 1278 CACGTG [CT]AC^GT[GA]
BsmBI 1650 GAGACG CGTCTC(1/5)
Bsp1407I 480 TGTACA T^GTACA
BsrFI 2287 GCCGGC [GA]^CCGG[CT]
BsrGI 480 TGTACA T^GTACA
BstEII 2249 GGTGACC G^GT[ACGT]ACC
BstPI 2249 GGTGACC G^GT[ACGT]ACC
BstYI 1288 AGATCT [GA]^GATC[CT]
CelII 1777 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2287 GCCGGC [GA]^CCGG[CT]
DraIII 1278 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1595 AGCGCT AGC^GCT
EcoO65I 2249 GGTGACC G^GT[ACGT]ACC
HindIII 1999 AAGCTT A^AGCTT
KasI 14 GGCGCC G^GCGCC
MfeI 2093 CAATTG C^AATTG
MflI 1288 AGATCT [GA]^GATC[CT]
MslI 799 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2093 CAATTG C^AATTG
NaeI 2287 GCCGGC GCC^GGC
NarI 14 GGCGCC GG^CGCC
NgoMIV 2287 GCCGGC G^CCGGC
PaeR7I 1200 CTCGAG C^TCGAG
PmaCI 1278 CACGTG CAC^GTG
PmlI 1278 CACGTG CAC^GTG
SfoI 14 GGCGCC GGC^GCC
SspBI 480 TGTACA T^GTACA
SspI 122 AATATT AAT^ATT
StuI 989 AGGCCT AGG^CCT
TaqI 1201 TCGA T^CGA
TthHB8I 1201 TCGA T^CGA
XcmI 2070 CCAGCCTGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1200 CTCGAG C^TCGAG
XhoII 1288 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 14 GGCGCC G[GA]^CG[CT]C
2139 GGCGTC
BglI 6 GCCCCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1699 GCCCAAGAGGC
BsaHI 14 GGCGCC G[GA]^CG[CT]C
2139 GGCGTC
BsiEI 44 CGGCCG CG[GA][CT]^CG
94 CGGCCG
BspMI 2010 ACCTGC ACCTGC(4/8)
901 GCAGGT
BsrBI 63 GAGCGG CCGCTC(-3/-3)
1988 GAGCGG
EagI 44 CGGCCG C^GGCCG
94 CGGCCG
EclXI 44 CGGCCG C^GGCCG
94 CGGCCG
Eco52I 44 CGGCCG C^GGCCG
94 CGGCCG
Hin1I 14 GGCGCC G[GA]^CG[CT]C
2139 GGCGTC
MaeII 892 ACGT A^CGT
1279 ACGT
PleI 2397 GACTC GAGTC(4/5)
1908 GAGTC
SmaI 1413 CCCGGG CCC^GGG
1855 CCCGGG
TfiI 186 GAATC G^A[AT]TC
2184 GATTC
XbaI 1749 TCTAGA T^CTAGA
1881 TCTAGA
XmaCI 1413 CCCGGG C^CCGGG
1855 CCCGGG
XmaI 1413 CCCGGG C^CCGGG
1855 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TseI Tsp45I Tsp509I
TspRI Van91I