##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaAI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BstBI ClaI DrdI Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SalI
SapI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AatII 3406 GACGTC GACGT^C
BamHI 3613 GGATCC G^GATCC
BsmBI 990 GAGACG CGTCTC(1/5)
BsrDI 715 GCAATG GCAATG(2/0)
BssSI 2278 CACGAG CACGAG(-5/-1)
Bst1107I 1346 GTATAC GTA^TAC
BstEII 2723 GGTCACC G^GT[ACGT]ACC
BstPI 2723 GGTCACC G^GT[ACGT]ACC
BstZ17I 1346 GTATAC GTA^TAC
CpoI 211 CGGTCCG CG^G[AT]CCG
EcoO65I 2723 GGTCACC G^GT[ACGT]ACC
HaeII 320 GGCGCT [GA]GCGC^[CT]
NaeI 711 GCCGGC GCC^GGC
NgoMIV 711 GCCGGC G^CCGGC
NheI 1851 GCTAGC G^CTAGC
PaeR7I 1807 CTCGAG C^TCGAG
RsrII 211 CGGTCCG CG^G[AT]CCG
SbfI 1840 CCTGCAGG CCTGCA^GG
SexAI 1360 ACCAGGT A^CC[AT]GGT
SfiI 449 GGCCCGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1405 CCCGGG CCC^GGG
Sse8387I 1840 CCTGCAGG CCTGCA^GG
StuI 1297 AGGCCT AGG^CCT
XcmI 772 CCACGGCTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1807 CTCGAG C^TCGAG
XmaCI 1405 CCCGGG C^CCGGG
XmaI 1405 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1346 GTATAC GT^[AC][GT]AC
3494 GTAGAC
AcyI 194 GACGCC G[GA]^CG[CT]C
3406 GACGTC
Asp700I 2336 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2423 GAAACTCTTC
BalI 2038 TGGCCA TGG^CCA
2191 TGGCCA
BbvCI 482 CCTCAGC CCTCAGC(-5/-2)
3122 GCTGAGG
BglII 970 AGATCT A^GATCT
1663 AGATCT
BlpI 416 GCTGAGC GC^T[ACGT]AGC
1110 GCTCAGC
Bpu1102I 416 GCTGAGC GC^T[ACGT]AGC
1110 GCTCAGC
BsaHI 194 GACGCC G[GA]^CG[CT]C
3406 GACGTC
Bsp1407I 799 TGTACA T^GTACA
1312 TGTACA
BsrBI 248 CCGCTC CCGCTC(-3/-3)
1071 GAGCGG
BsrGI 799 TGTACA T^GTACA
1312 TGTACA
BssHII 266 GCGCGC G^CGCGC
268 GCGCGC
BstAPI 1547 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1688 GCAGAACCTGC
BstXI 772 CCACGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2243 CCAGCGTGATGG
CelII 416 GCTGAGC GC^T[ACGT]AGC
1110 GCTCAGC
DraI 2943 TTTAAA TTT^AAA
3742 TTTAAA
DraIII 1359 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1548 CACCGTGTG
EagI 646 CGGCCG C^GGCCG
1377 CGGCCG
EarI 2427 CTCTTC CTCTTC(1/4)
2603 GAAGAG
EclXI 646 CGGCCG C^GGCCG
1377 CGGCCG
Eco52I 646 CGGCCG C^GGCCG
1377 CGGCCG
EcoNI 2432 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3295 CCTGTATCAGG
EcoRI 1931 GAATTC G^AATTC
2340 GAATTC
Hin1I 194 GACGCC G[GA]^CG[CT]C
3406 GACGTC
Ksp632I 2427 CTCTTC CTCTTC(1/4)
2603 GAAGAG
KspI 139 CCGCGG CCGC^GG
1374 CCGCGG
MluNI 2038 TGGCCA TGG^CCA
2191 TGGCCA
MscI 2038 TGGCCA TGG^CCA
2191 TGGCCA
NcoI 1709 CCATGG C^CATGG
1884 CCATGG
SacII 139 CCGCGG CCGC^GG
1374 CCGCGG
SspBI 799 TGTACA T^GTACA
1312 TGTACA
XmnI 2336 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2423 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp718I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII