##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AviII BbrPI BciVI
BclI BfrI BglII BsaAI BsaBI
BsaWI BseAI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI FbaI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1630 GTCTAC GT^[AC][GT]AC
AhdI 2680 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2090 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1117 AGCGCT AGC^GCT
ApaI 1106 GGGCCC GGGCC^C
AseI 631 ATTAAT AT^TAAT
AsnI 631 ATTAAT AT^TAAT
AspEI 2680 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2585 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2199 CCTAGG C^CTAGG
BalI 407 TGGCCA TGG^CCA
BamHI 2417 GGATCC G^GATCC
BbvCI 2302 CCTCAGC CCTCAGC(-5/-2)
BcgI 1152 CGAGAGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2199 CCTAGG C^CTAGG
BsaI 589 GAGACC GGTCTC(1/5)
BsiWI 500 CGTACG C^GTACG
BsmI 1230 GAATGC GAATGC(1/-1)
BsrDI 2666 GCAATG GCAATG(2/0)
BssSI 1087 CACGAG CACGAG(-5/-1)
BstEII 2084 GGTGACC G^GT[ACGT]ACC
BstPI 2084 GGTGACC G^GT[ACGT]ACC
BstXI 410 CCAATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2090 CCTTAGG CC^T[ACGT]AGG
DraIII 1010 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2680 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1117 AGCGCT AGC^GCT
Eco81I 2090 CCTTAGG CC^T[ACGT]AGG
EcoNI 951 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2084 GGTGACC G^GT[ACGT]ACC
EcoRI 1291 GAATTC G^AATTC
EcoRV 1842 GATATC GAT^ATC
EcoT22I 2019 ATGCAT ATGCA^T
FseI 331 GGCCGGCC GGCCGG^CC
MaeII 1667 ACGT A^CGT
MluNI 407 TGGCCA TGG^CCA
MscI 407 TGGCCA TGG^CCA
NsiI 2019 ATGCAT ATGCA^T
NspI 310 GCATGC [GA]CATG^[CT]
PaeR7I 389 CTCGAG C^TCGAG
PflFI 2585 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1347 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 631 ATTAAT AT^TAAT
SacI 246 GAGCTC GAGCT^C
SexAI 1796 ACCTGGT A^CC[AT]GGT
SphI 310 GCATGC GCATG^C
StuI 2061 AGGCCT AGG^CCT
TfiI 2008 GATTC G^A[AT]TC
Tth111I 2585 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1347 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2250 TCTAGA T^CTAGA
XhoI 389 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1291 GAATTC [GA]^AATT[CT]
3078 GAATTT
ApoI 1291 GAATTC [GA]^AATT[CT]
3078 GAATTT
BbeI 139 GGCGCC GGCGC^C
368 GGCGCC
BbsI 1284 GTCTTC GAAGAC(2/6)
1608 GTCTTC
BglI 27 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
853 GCCGTGGTGGC
BpuAI 1284 GTCTTC GAAGAC(2/6)
1608 GTCTTC
BspHI 1575 TCATGA T^CATGA
1918 TCATGA
BspMI 482 ACCTGC ACCTGC(4/8)
946 ACCTGC
BsrFI 332 GCCGGC [GA]^CCGG[CT]
767 GCCGGC
Cfr10I 332 GCCGGC [GA]^CCGG[CT]
767 GCCGGC
KasI 139 GGCGCC G^GCGCC
368 GGCGCC
NaeI 332 GCCGGC GCC^GGC
767 GCCGGC
NarI 139 GGCGCC GG^CGCC
368 GGCGCC
NgoMIV 332 GCCGGC G^CCGGC
767 GCCGGC
PvuII 669 CAGCTG CAG^CTG
895 CAGCTG
RcaI 1575 TCATGA T^CATGA
1918 TCATGA
SapI 642 GAAGAGC GCTCTTC(1/4)
2337 GAAGAGC
SfoI 139 GGCGCC GGC^GCC
368 GGCGCC
SmaI 113 CCCGGG CCC^GGG
1032 CCCGGG
SmlI 389 CTCGAG C^T[CT][GA]AG
583 CTCAAG
XmaCI 113 CCCGGG C^CCGGG
1032 CCCGGG
XmaI 113 CCCGGG C^CCGGG
1032 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII