##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI BbvCI BclI BfrI BglII
BlpI Bpu1102I BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII Eam1105I EcoO65I
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MamI MluI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 578 GACGTC GACGT^C
AccIII 2187 TCCGGA T^CCGGA
AflIII 3371 ACATGT A^C[GA][CT]GT
Alw44I 3324 GTGCAC G^TGCAC
AocI 3004 CCTCAGG CC^T[ACGT]AGG
Aor51HI 128 AGCGCT AGC^GCT
ApaLI 3324 GTGCAC G^TGCAC
AviII 2252 TGCGCA TGC^GCA
BamHI 1640 GGATCC G^GATCC
BbeI 1416 GGCGCC GGCGC^C
BbrPI 1663 CACGTG CAC^GTG
BbsI 660 GTCTTC GAAGAC(2/6)
BcgI 1704 CGACAGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1260 GTATCC GTATCC(6/5)
BpuAI 660 GTCTTC GAAGAC(2/6)
BseAI 2187 TCCGGA T^CCGGA
BspEI 2187 TCCGGA T^CCGGA
BspLU11I 3371 ACATGT A^CATGT
BssHII 269 GCGCGC G^CGCGC
BssSI 916 CACGAG CACGAG(-5/-1)
BstXI 897 CCATGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1640 GGATCC [GA]^GATC[CT]
Bsu36I 3004 CCTCAGG CC^T[ACGT]AGG
DpnII 1641 GATC ^GATC
DraI 2557 TTTAAA TTT^AAA
DrdI 940 GACCGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 128 AGCGCT AGC^GCT
Eco81I 3004 CCTCAGG CC^T[ACGT]AGG
EcoRI 1492 GAATTC G^AATTC
FseI 276 GGCCGGCC GGCCGG^CC
FspI 2252 TGCGCA TGC^GCA
HincII 1873 GTCAAC GT[CT]^[GA]AC
HindII 1873 GTCAAC GT[CT]^[GA]AC
KasI 1416 GGCGCC G^GCGCC
KspI 1914 CCGCGG CCGC^GG
MboI 1641 GATC ^GATC
MfeI 2666 CAATTG C^AATTG
MflI 1640 GGATCC [GA]^GATC[CT]
MroI 2187 TCCGGA T^CCGGA
MunI 2666 CAATTG C^AATTG
NarI 1416 GGCGCC GG^CGCC
NcoI 1346 CCATGG C^CATGG
NdeII 1641 GATC ^GATC
PmaCI 1663 CACGTG CAC^GTG
PmlI 1663 CACGTG CAC^GTG
SacII 1914 CCGCGG CCGC^GG
Sau3AI 1641 GATC ^GATC
SbfI 2220 CCTGCAGG CCTGCA^GG
SfiI 3000 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1416 GGCGCC GGC^GCC
SpeI 510 ACTAGT A^CTAGT
SphI 196 GCATGC GCATG^C
Sse8387I 2220 CCTGCAGG CCTGCA^GG
TaqI 2156 TCGA T^CGA
TthHB8I 2156 TCGA T^CGA
XcmI 1227 CCAAGACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1640 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 635 GAATTT [GA]^AATT[CT]
1492 GAATTC
AlwI 1641 GATCC GGATC(4/5)
1640 GGATC
ApaI 831 GGGCCC GGGCC^C
3034 GGGCCC
ApoI 635 GAATTT [GA]^AATT[CT]
1492 GAATTC
Asp700I 481 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1071 GAACACATTC
AspI 2714 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3205 GACTTCGTC
BglI 2521 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3001 GCCCCTCAGGC
BsaAI 811 TACGTG [CT]AC^GT[GA]
1663 CACGTG
BsaWI 587 ACCGGA [AT]^CCGG[AT]
2187 TCCGGA
BsmBI 1464 CGTCTC CGTCTC(1/5)
1836 CGTCTC
BspMI 2223 GCAGGT ACCTGC(4/8)
3114 GCAGGT
BsrDI 383 CATTGC GCAATG(2/0)
2168 GCAATG
BstAPI 1622 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2288 GCAAGTTCTGC
EagI 275 CGGCCG C^GGCCG
279 CGGCCG
EclXI 275 CGGCCG C^GGCCG
279 CGGCCG
Eco52I 275 CGGCCG C^GGCCG
279 CGGCCG
NaeI 273 GCCGGC GCC^GGC
277 GCCGGC
NgoMIV 273 GCCGGC G^CCGGC
277 GCCGGC
PflFI 2714 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3205 GACTTCGTC
PflMI 885 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1207 CCAGCCTGTGG
PshAI 1996 GACAAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3391 GACCCCAGTC
SacI 333 GAGCTC GAGCT^C
2453 GAGCTC
SmlI 404 CTTGAG C^T[CT][GA]AG
1828 CTCAAG
TfiI 2027 GATTC G^A[AT]TC
2984 GAATC
Tth111I 2714 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3205 GACTTCGTC
Van91I 885 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1207 CCAGCCTGTGG
XmnI 481 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1071 GAACACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII AvrII
BalI BanI BanII BbvI BcnI
BfaI BlnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI StuI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI