##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccII AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BcgI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshBI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SgrAI SnaBI Sse8387I SspI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1111 GGTACC G^GTACC
AclI 1061 AACGTT AA^CGTT
AcyI 545 GGCGCC G[GA]^CG[CT]C
AflIII 561 ACGTGT A^C[GA][CT]GT
AlwNI 1663 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2142 CCTGAGG CC^T[ACGT]AGG
Asp718I 1111 GGTACC G^GTACC
BalI 1455 TGGCCA TGG^CCA
BbeI 545 GGCGCC GGCGC^C
BbrPI 560 CACGTG CAC^GTG
BbvCI 1581 CCTCAGC CCTCAGC(-5/-2)
BciVI 6 GGATAC GTATCC(6/5)
BglI 1903 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 560 CACGTG [CT]AC^GT[GA]
BsaHI 545 GGCGCC G[GA]^CG[CT]C
BsaWI 446 TCCGGT [AT]^CCGG[AT]
BsiEI 308 CGGTCG CG[GA][CT]^CG
BsmBI 1419 CGTCTC CGTCTC(1/5)
BsrFI 522 GCCGGT [GA]^CCGG[CT]
BstAPI 313 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1176 GGTGACC G^GT[ACGT]ACC
BstPI 1176 GGTGACC G^GT[ACGT]ACC
BstXI 186 CCATGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2142 CCTGAGG CC^T[ACGT]AGG
Cfr10I 522 GCCGGT [GA]^CCGG[CT]
DraI 2041 TTTAAA TTT^AAA
Eco81I 2142 CCTGAGG CC^T[ACGT]AGG
EcoNI 287 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1176 GGTGACC G^GT[ACGT]ACC
EcoRI 1849 GAATTC G^AATTC
HaeII 545 GGCGCC [GA]GCGC^[CT]
Hin1I 545 GGCGCC G[GA]^CG[CT]C
KasI 545 GGCGCC G^GCGCC
KpnI 1111 GGTACC GGTAC^C
MluNI 1455 TGGCCA TGG^CCA
MscI 1455 TGGCCA TGG^CCA
MseI 2042 TTAA T^TAA
NarI 545 GGCGCC GG^CGCC
PflMI 135 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 560 CACGTG CAC^GTG
PmlI 560 CACGTG CAC^GTG
PpuMI 1121 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2092 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1061 AACGTT AA^CGTT
SfoI 545 GGCGCC GGC^GCC
SmaI 1484 CCCGGG CCC^GGG
SpeI 616 ACTAGT A^CTAGT
SphI 797 GCATGC GCATG^C
Tru9I 2042 TTAA T^TAA
Van91I 135 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 124 TCTAGA T^CTAGA
XmaCI 1484 CCCGGG C^CCGGG
XmaI 1484 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 668 GAATTT [GA]^AATT[CT]
1849 GAATTC
ApoI 668 GAATTT [GA]^AATT[CT]
1849 GAATTC
Asp700I 88 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAACGTTTC
BbsI 958 GAAGAC GAAGAC(2/6)
1533 GAAGAC
BpuAI 958 GAAGAC GAAGAC(2/6)
1533 GAAGAC
BsgI 683 GTGCAG GTGCAG(16/14)
1980 GTGCAG
BsrBI 647 GAGCGG CCGCTC(-3/-3)
2178 GAGCGG
BstBI 95 TTCGAA TT^CGAA
1244 TTCGAA
EaeI 1251 CGGCCA [CT]^GGCC[GA]
1455 TGGCCA
HindIII 1051 AAGCTT A^AGCTT
1240 AAGCTT
MaeII 561 ACGT A^CGT
1062 ACGT
NspI 797 GCATGC [GA]CATG^[CT]
1564 ACATGC
NspV 95 TTCGAA TT^CGAA
1244 TTCGAA
SapI 2008 GAAGAGC GCTCTTC(1/4)
1323 GCTCTTC
SfuI 95 TTCGAA TT^CGAA
1244 TTCGAA
StuI 690 AGGCCT AGG^CCT
1520 AGGCCT
XcmI 1163 CCAATGTCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1644 CCAGCGGCTAATTGG
XmnI 88 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1059 GAAACGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PaeR7I PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoI XhoII