################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AccI AccII AccIII AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MamI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1111 GGTACC G^GTACC AclI 1061 AACGTT AA^CGTT AcyI 545 GGCGCC G[GA]^CG[CT]C AflIII 561 ACGTGT A^C[GA][CT]GT AlwNI 1663 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2142 CCTGAGG CC^T[ACGT]AGG Asp718I 1111 GGTACC G^GTACC BalI 1455 TGGCCA TGG^CCA BbeI 545 GGCGCC GGCGC^C BbrPI 560 CACGTG CAC^GTG BbvCI 1581 CCTCAGC CCTCAGC(-5/-2) BciVI 6 GGATAC GTATCC(6/5) BglI 1903 GCCCGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 560 CACGTG [CT]AC^GT[GA] BsaHI 545 GGCGCC G[GA]^CG[CT]C BsaWI 446 TCCGGT [AT]^CCGG[AT] BsiEI 308 CGGTCG CG[GA][CT]^CG BsmBI 1419 CGTCTC CGTCTC(1/5) BsrFI 522 GCCGGT [GA]^CCGG[CT] BstAPI 313 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1176 GGTGACC G^GT[ACGT]ACC BstPI 1176 GGTGACC G^GT[ACGT]ACC BstXI 186 CCATGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2142 CCTGAGG CC^T[ACGT]AGG Cfr10I 522 GCCGGT [GA]^CCGG[CT] DraI 2041 TTTAAA TTT^AAA Eco81I 2142 CCTGAGG CC^T[ACGT]AGG EcoNI 287 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1176 GGTGACC G^GT[ACGT]ACC EcoRI 1849 GAATTC G^AATTC HaeII 545 GGCGCC [GA]GCGC^[CT] Hin1I 545 GGCGCC G[GA]^CG[CT]C KasI 545 GGCGCC G^GCGCC KpnI 1111 GGTACC GGTAC^C MluNI 1455 TGGCCA TGG^CCA MscI 1455 TGGCCA TGG^CCA MseI 2042 TTAA T^TAA NarI 545 GGCGCC GG^CGCC PflMI 135 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 560 CACGTG CAC^GTG PmlI 560 CACGTG CAC^GTG PpuMI 1121 AGGACCC [GA]G^G[AT]CC[CT] PshAI 2092 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1061 AACGTT AA^CGTT SfoI 545 GGCGCC GGC^GCC SmaI 1484 CCCGGG CCC^GGG SpeI 616 ACTAGT A^CTAGT SphI 797 GCATGC GCATG^C Tru9I 2042 TTAA T^TAA Van91I 135 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 124 TCTAGA T^CTAGA XmaCI 1484 CCCGGG C^CCGGG XmaI 1484 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 668 GAATTT [GA]^AATT[CT] 1849 GAATTC ApoI 668 GAATTT [GA]^AATT[CT] 1849 GAATTC Asp700I 88 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1059 GAAACGTTTC BbsI 958 GAAGAC GAAGAC(2/6) 1533 GAAGAC BpuAI 958 GAAGAC GAAGAC(2/6) 1533 GAAGAC BsgI 683 GTGCAG GTGCAG(16/14) 1980 GTGCAG BsrBI 647 GAGCGG CCGCTC(-3/-3) 2178 GAGCGG BstBI 95 TTCGAA TT^CGAA 1244 TTCGAA EaeI 1251 CGGCCA [CT]^GGCC[GA] 1455 TGGCCA HindIII 1051 AAGCTT A^AGCTT 1240 AAGCTT MaeII 561 ACGT A^CGT 1062 ACGT NspI 797 GCATGC [GA]CATG^[CT] 1564 ACATGC NspV 95 TTCGAA TT^CGAA 1244 TTCGAA SapI 2008 GAAGAGC GCTCTTC(1/4) 1323 GCTCTTC SfuI 95 TTCGAA TT^CGAA 1244 TTCGAA StuI 690 AGGCCT AGG^CCT 1520 AGGCCT XcmI 1163 CCAATGTCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1644 CCAGCGGCTAATTGG XmnI 88 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1059 GAAACGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PaeR7I PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XhoII