##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII BbeI BcgI
BfrI BglII BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PacI PaeR7I PmeI PshBI
PvuI PvuII RcaI RsrII SacII
SalI SexAI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2317 GTCTAC GT^[AC][GT]AC
AccII 182 CGCG CG^CG
AclI 2559 AACGTT AA^CGTT
AcyI 888 GACGCC G[GA]^CG[CT]C
AgeI 1553 ACCGGT A^CCGGT
Alw44I 177 GTGCAC G^TGCAC
ApaLI 177 GTGCAC G^TGCAC
Asp700I 815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1703 CCTAGG C^CTAGG
BanI 1110 GGTGCC G^G[CT][GA]CC
BbrPI 2422 CACGTG CAC^GTG
BclI 2008 TGATCA T^GATCA
BlnI 1703 CCTAGG C^CTAGG
BlpI 619 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 619 GCTCAGC GC^T[ACGT]AGC
BsaAI 2422 CACGTG [CT]AC^GT[GA]
BsaHI 888 GACGCC G[GA]^CG[CT]C
BsiEI 1551 CGACCG CG[GA][CT]^CG
BsmBI 651 CGTCTC CGTCTC(1/5)
BspLU11I 1975 ACATGT A^CATGT
BspMI 1576 ACCTGC ACCTGC(4/8)
BsrFI 1553 ACCGGT [GA]^CCGG[CT]
BstUI 182 CGCG CG^CG
CelII 619 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1553 ACCGGT [GA]^CCGG[CT]
DraIII 1304 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1353 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1203 ATGCAT ATGCA^T
FbaI 2008 TGATCA T^GATCA
Hin1I 888 GACGCC G[GA]^CG[CT]C
MfeI 2487 CAATTG C^AATTG
MunI 2487 CAATTG C^AATTG
MvnI 182 CGCG CG^CG
NcoI 802 CCATGG C^CATGG
NdeI 2527 CATATG CA^TATG
NsiI 1203 ATGCAT ATGCA^T
PflFI 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1553 ACCGGT A^CCGGT
PmaCI 2422 CACGTG CAC^GTG
PmlI 2422 CACGTG CAC^GTG
PshAI 107 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2559 AACGTT AA^CGTT
SacI 2251 GAGCTC GAGCT^C
SapI 1802 GAAGAGC GCTCTTC(1/4)
ScaI 2466 AGTACT AGT^ACT
TfiI 2409 GAATC G^A[AT]TC
Tth111I 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1975 ACATGT A^C[GA][CT]GT
2423 ACGTGT
AspHI 177 GTGCAC G[AT]GC[AT]^C
2251 GAGCTC
AvaI 1161 CCCGAG C^[CT]CG[GA]G
1621 CTCGGG
BamHI 1715 GGATCC G^GATCC
2260 GGATCC
BbsI 773 GAAGAC GAAGAC(2/6)
1257 GTCTTC
BbvCI 2277 CCTCAGC CCTCAGC(-5/-2)
1271 GCTGAGG
BciVI 388 GTATCC GTATCC(6/5)
2301 GTATCC
BpuAI 773 GAAGAC GAAGAC(2/6)
1257 GTCTTC
BsaWI 1553 ACCGGT [AT]^CCGG[AT]
2147 ACCGGA
BsgI 640 GTGCAG GTGCAG(16/14)
1368 GTGCAG
BsiHKAI 177 GTGCAC G[AT]GC[AT]^C
2251 GAGCTC
BsoBI 1161 CCCGAG C^[CT]CG[GA]G
1621 CTCGGG
BstXI 353 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
796 CCAGCCCCATGG
DraI 2019 TTTAAA TTT^AAA
2581 TTTAAA
EarI 1584 CTCTTC CTCTTC(1/4)
1802 GAAGAG
EcoRI 1482 GAATTC G^AATTC
2587 GAATTC
HaeII 88 GGCGCT [GA]GCGC^[CT]
931 AGCGCC
HgaI 888 GACGC GACGC(5/10)
983 GACGC
Ksp632I 1584 CTCTTC CTCTTC(1/4)
1802 GAAGAG
MspA1I 622 CAGCGG C[AC]G^C[GT]G
1114 CCGCTG
NheI 1155 GCTAGC G^CTAGC
1730 GCTAGC
PflMI 509 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1403 CCAGGCACTGG
SbfI 1763 CCTGCAGG CCTGCA^GG
1958 CCTGCAGG
SfiI 818 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1559 GGCCCAAAAGGCC
SphI 29 GCATGC GCATG^C
2139 GCATGC
Sse8387I 1763 CCTGCAGG CCTGCA^GG
1958 CCTGCAGG
TaqI 1238 TCGA T^CGA
1298 TCGA
TthHB8I 1238 TCGA T^CGA
1298 TCGA
Van91I 509 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1403 CCAGGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaII BalI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII