################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccIII AflII AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII BbeI BcgI BfrI BglII BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PmeI PshBI PvuI PvuII RcaI RsrII SacII SalI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 2317 GTCTAC GT^[AC][GT]AC AccII 182 CGCG CG^CG AclI 2559 AACGTT AA^CGTT AcyI 888 GACGCC G[GA]^CG[CT]C AgeI 1553 ACCGGT A^CCGGT Alw44I 177 GTGCAC G^TGCAC ApaLI 177 GTGCAC G^TGCAC Asp700I 815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1703 CCTAGG C^CTAGG BanI 1110 GGTGCC G^G[CT][GA]CC BbrPI 2422 CACGTG CAC^GTG BclI 2008 TGATCA T^GATCA BlnI 1703 CCTAGG C^CTAGG BlpI 619 GCTCAGC GC^T[ACGT]AGC Bpu1102I 619 GCTCAGC GC^T[ACGT]AGC BsaAI 2422 CACGTG [CT]AC^GT[GA] BsaHI 888 GACGCC G[GA]^CG[CT]C BsiEI 1551 CGACCG CG[GA][CT]^CG BsmBI 651 CGTCTC CGTCTC(1/5) BspLU11I 1975 ACATGT A^CATGT BspMI 1576 ACCTGC ACCTGC(4/8) BsrFI 1553 ACCGGT [GA]^CCGG[CT] BstUI 182 CGCG CG^CG CelII 619 GCTCAGC GC^T[ACGT]AGC Cfr10I 1553 ACCGGT [GA]^CCGG[CT] DraIII 1304 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1353 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1203 ATGCAT ATGCA^T FbaI 2008 TGATCA T^GATCA Hin1I 888 GACGCC G[GA]^CG[CT]C MfeI 2487 CAATTG C^AATTG MunI 2487 CAATTG C^AATTG MvnI 182 CGCG CG^CG NcoI 802 CCATGG C^CATGG NdeI 2527 CATATG CA^TATG NsiI 1203 ATGCAT ATGCA^T PflFI 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1553 ACCGGT A^CCGGT PmaCI 2422 CACGTG CAC^GTG PmlI 2422 CACGTG CAC^GTG PshAI 107 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2559 AACGTT AA^CGTT SacI 2251 GAGCTC GAGCT^C SapI 1802 GAAGAGC GCTCTTC(1/4) ScaI 2466 AGTACT AGT^ACT TfiI 2409 GAATC G^A[AT]TC Tth111I 713 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 815 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AflIII 1975 ACATGT A^C[GA][CT]GT 2423 ACGTGT AspHI 177 GTGCAC G[AT]GC[AT]^C 2251 GAGCTC AvaI 1161 CCCGAG C^[CT]CG[GA]G 1621 CTCGGG BamHI 1715 GGATCC G^GATCC 2260 GGATCC BbsI 773 GAAGAC GAAGAC(2/6) 1257 GTCTTC BbvCI 2277 CCTCAGC CCTCAGC(-5/-2) 1271 GCTGAGG BciVI 388 GTATCC GTATCC(6/5) 2301 GTATCC BpuAI 773 GAAGAC GAAGAC(2/6) 1257 GTCTTC BsaWI 1553 ACCGGT [AT]^CCGG[AT] 2147 ACCGGA BsgI 640 GTGCAG GTGCAG(16/14) 1368 GTGCAG BsiHKAI 177 GTGCAC G[AT]GC[AT]^C 2251 GAGCTC BsoBI 1161 CCCGAG C^[CT]CG[GA]G 1621 CTCGGG BstXI 353 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 796 CCAGCCCCATGG DraI 2019 TTTAAA TTT^AAA 2581 TTTAAA EarI 1584 CTCTTC CTCTTC(1/4) 1802 GAAGAG EcoRI 1482 GAATTC G^AATTC 2587 GAATTC HaeII 88 GGCGCT [GA]GCGC^[CT] 931 AGCGCC HgaI 888 GACGC GACGC(5/10) 983 GACGC Ksp632I 1584 CTCTTC CTCTTC(1/4) 1802 GAAGAG MspA1I 622 CAGCGG C[AC]G^C[GT]G 1114 CCGCTG NheI 1155 GCTAGC G^CTAGC 1730 GCTAGC PflMI 509 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1403 CCAGGCACTGG SbfI 1763 CCTGCAGG CCTGCA^GG 1958 CCTGCAGG SfiI 818 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1559 GGCCCAAAAGGCC SphI 29 GCATGC GCATG^C 2139 GCATGC Sse8387I 1763 CCTGCAGG CCTGCA^GG 1958 CCTGCAGG TaqI 1238 TCGA T^CGA 1298 TCGA TthHB8I 1238 TCGA T^CGA 1298 TCGA Van91I 509 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1403 CCAGGCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AvaII BalI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII