##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
Aor51HI ApaI AscI Asp718I AspI
AvaI BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BglI BglII
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstBI BstYI BstZ17I CelII Cfr10I
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I EcoT22I FseI HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I StuI
SwaI Tth111I XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AclI 2276 AACGTT AA^CGTT
AcyI 2345 GACGCC G[GA]^CG[CT]C
AflII 1491 CTTAAG C^TTAAG
Alw44I 1063 GTGCAC G^TGCAC
AlwI 272 GGATC GGATC(4/5)
AocI 2493 CCTGAGG CC^T[ACGT]AGG
ApaLI 1063 GTGCAC G^TGCAC
AviII 2245 TGCGCA TGC^GCA
AvrII 752 CCTAGG C^CTAGG
BbsI 2676 GAAGAC GAAGAC(2/6)
BclI 2684 TGATCA T^GATCA
BfrI 1491 CTTAAG C^TTAAG
BlnI 752 CCTAGG C^CTAGG
BpmI 2670 CTGGAG CTGGAG(16/14)
BpuAI 2676 GAAGAC GAAGAC(2/6)
BsaHI 2345 GACGCC G[GA]^CG[CT]C
BsaI 257 GGTCTC GGTCTC(1/5)
BsaWI 180 ACCGGA [AT]^CCGG[AT]
BsmI 1654 GAATGC GAATGC(1/-1)
BspDI 73 ATCGAT AT^CGAT
BspLU11I 1276 ACATGT A^CATGT
BspMI 774 GCAGGT ACCTGC(4/8)
BstAPI 2547 GCATAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1221 GGTGACC G^GT[ACGT]ACC
BstPI 1221 GGTGACC G^GT[ACGT]ACC
BstXI 1167 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2493 CCTGAGG CC^T[ACGT]AGG
ClaI 73 ATCGAT AT^CGAT
DraII 2211 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2358 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1837 CCATGG C^C[GA][CT]GG
Eco81I 2493 CCTGAGG CC^T[ACGT]AGG
EcoNI 2625 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2211 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1221 GGTGACC G^GT[ACGT]ACC
EcoRI 833 GAATTC G^AATTC
EcoRV 405 GATATC GAT^ATC
FauI 498 CCCGC CCCGC(4/6)
FbaI 2684 TGATCA T^GATCA
FspI 2245 TGCGCA TGC^GCA
HaeII 5 AGCGCC [GA]GCGC^[CT]
HgaI 2345 GACGC GACGC(5/10)
Hin1I 2345 GACGCC G[GA]^CG[CT]C
HindIII 974 AAGCTT A^AGCTT
NcoI 1837 CCATGG C^CATGG
PflMI 2357 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2211 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2276 AACGTT AA^CGTT
SexAI 1128 ACCAGGT A^CC[AT]GGT
SnaBI 474 TACGTA TAC^GTA
SphI 1233 GCATGC GCATG^C
TfiI 120 GAATC G^A[AT]TC
Van91I 2357 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 168 TCTAGA T^CTAGA
XcmI 1167 CCAGCTCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 30 CGCG CG^CG
451 CGCG
AcsI 833 GAATTC [GA]^AATT[CT]
920 GAATTT
AflIII 1276 ACATGT A^C[GA][CT]GT
1665 ACGTGT
AhdI 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2324 GACACGGAGTC
AlwNI 1809 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2170 CAGTGTCTG
ApoI 833 GAATTC [GA]^AATT[CT]
920 GAATTT
Asp700I 160 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1315 GAACCCTTTC
AspEI 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2324 GACACGGAGTC
BanI 748 GGTGCC G^G[CT][GA]CC
1611 GGCACC
BanII 493 GGGCTC G[GA]GC[CT]^C
1343 GAGCCC
BsaAI 474 TACGTA [CT]AC^GT[GA]
2019 TACGTG
Bsp1407I 1577 TGTACA T^GTACA
2907 TGTACA
BsrGI 1577 TGTACA T^GTACA
2907 TGTACA
BssSI 91 CTCGTG CACGAG(-5/-1)
1692 CTCGTG
BstUI 30 CGCG CG^CG
451 CGCG
Eam1105I 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2324 GACACGGAGTC
EcoT14I 752 CCTAGG C^C[AT][AT]GG
1837 CCATGG
HaeIII 156 GGCC GG^CC
1798 GGCC
HincII 1272 GTTGAC GT[CT]^[GA]AC
1405 GTTGAC
HindII 1272 GTTGAC GT[CT]^[GA]AC
1405 GTTGAC
MvnI 30 CGCG CG^CG
451 CGCG
PvuII 668 CAGCTG CAG^CTG
1809 CAGCTG
SspBI 1577 TGTACA T^GTACA
2907 TGTACA
SspI 865 AATATT AAT^ATT
2784 AATATT
StyI 752 CCTAGG C^C[AT][AT]GG
1837 CCATGG
XmnI 160 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1315 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AseI AsnI
AspHI AvaII BbvI BcnI BfaI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I