################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AgeI Aor51HI ApaI AscI Asp718I AspI AvaI BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BglI BglII BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspHI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstYI BstZ17I CelII Cfr10I CpoI DrdI EaeI EagI EclXI Eco47III Eco52I EcoT22I FseI HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I StuI SwaI Tth111I XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AclI 2276 AACGTT AA^CGTT AcyI 2345 GACGCC G[GA]^CG[CT]C AflII 1491 CTTAAG C^TTAAG Alw44I 1063 GTGCAC G^TGCAC AlwI 272 GGATC GGATC(4/5) AocI 2493 CCTGAGG CC^T[ACGT]AGG ApaLI 1063 GTGCAC G^TGCAC AviII 2245 TGCGCA TGC^GCA AvrII 752 CCTAGG C^CTAGG BbsI 2676 GAAGAC GAAGAC(2/6) BclI 2684 TGATCA T^GATCA BfrI 1491 CTTAAG C^TTAAG BlnI 752 CCTAGG C^CTAGG BpmI 2670 CTGGAG CTGGAG(16/14) BpuAI 2676 GAAGAC GAAGAC(2/6) BsaHI 2345 GACGCC G[GA]^CG[CT]C BsaI 257 GGTCTC GGTCTC(1/5) BsaWI 180 ACCGGA [AT]^CCGG[AT] BsmI 1654 GAATGC GAATGC(1/-1) BspDI 73 ATCGAT AT^CGAT BspLU11I 1276 ACATGT A^CATGT BspMI 774 GCAGGT ACCTGC(4/8) BstAPI 2547 GCATAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1221 GGTGACC G^GT[ACGT]ACC BstPI 1221 GGTGACC G^GT[ACGT]ACC BstXI 1167 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2493 CCTGAGG CC^T[ACGT]AGG ClaI 73 ATCGAT AT^CGAT DraII 2211 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 2358 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1837 CCATGG C^C[GA][CT]GG Eco81I 2493 CCTGAGG CC^T[ACGT]AGG EcoNI 2625 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2211 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 1221 GGTGACC G^GT[ACGT]ACC EcoRI 833 GAATTC G^AATTC EcoRV 405 GATATC GAT^ATC FauI 498 CCCGC CCCGC(4/6) FbaI 2684 TGATCA T^GATCA FspI 2245 TGCGCA TGC^GCA HaeII 5 AGCGCC [GA]GCGC^[CT] HgaI 2345 GACGC GACGC(5/10) Hin1I 2345 GACGCC G[GA]^CG[CT]C HindIII 974 AAGCTT A^AGCTT NcoI 1837 CCATGG C^CATGG PflMI 2357 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2211 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 2276 AACGTT AA^CGTT SexAI 1128 ACCAGGT A^CC[AT]GGT SnaBI 474 TACGTA TAC^GTA SphI 1233 GCATGC GCATG^C TfiI 120 GAATC G^A[AT]TC Van91I 2357 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 168 TCTAGA T^CTAGA XcmI 1167 CCAGCTCAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 30 CGCG CG^CG 451 CGCG AcsI 833 GAATTC [GA]^AATT[CT] 920 GAATTT AflIII 1276 ACATGT A^C[GA][CT]GT 1665 ACGTGT AhdI 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2324 GACACGGAGTC AlwNI 1809 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 2170 CAGTGTCTG ApoI 833 GAATTC [GA]^AATT[CT] 920 GAATTT Asp700I 160 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1315 GAACCCTTTC AspEI 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2324 GACACGGAGTC BanI 748 GGTGCC G^G[CT][GA]CC 1611 GGCACC BanII 493 GGGCTC G[GA]GC[CT]^C 1343 GAGCCC BsaAI 474 TACGTA [CT]AC^GT[GA] 2019 TACGTG Bsp1407I 1577 TGTACA T^GTACA 2907 TGTACA BsrGI 1577 TGTACA T^GTACA 2907 TGTACA BssSI 91 CTCGTG CACGAG(-5/-1) 1692 CTCGTG BstUI 30 CGCG CG^CG 451 CGCG Eam1105I 1664 GACGTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2324 GACACGGAGTC EcoT14I 752 CCTAGG C^C[AT][AT]GG 1837 CCATGG HaeIII 156 GGCC GG^CC 1798 GGCC HincII 1272 GTTGAC GT[CT]^[GA]AC 1405 GTTGAC HindII 1272 GTTGAC GT[CT]^[GA]AC 1405 GTTGAC MvnI 30 CGCG CG^CG 451 CGCG PvuII 668 CAGCTG CAG^CTG 1809 CAGCTG SspBI 1577 TGTACA T^GTACA 2907 TGTACA SspI 865 AATATT AAT^ATT 2784 AATATT StyI 752 CCTAGG C^C[AT][AT]GG 1837 CCATGG XmnI 160 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1315 GAACCCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AseI AsnI AspHI AvaII BbvI BcnI BfaI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I