##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AgeI        
Aor51HI     ApaI        AscI        Asp718I     AspI        
AvaI        BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BglI        BglII       
BlpI        Bpu1102I    BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspEI       BspHI       
BsrBI       BsrDI       BsrFI       BssHII      Bst1107I    
BstBI       BstYI       BstZ17I     CelII       Cfr10I      
CpoI        DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      EcoT22I     FseI        HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SpeI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AclI         2276  AACGTT               AA^CGTT
AcyI         2345  GACGCC               G[GA]^CG[CT]C
AflII        1491  CTTAAG               C^TTAAG
Alw44I       1063  GTGCAC               G^TGCAC
AlwI          272  GGATC                GGATC(4/5)
AocI         2493  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1063  GTGCAC               G^TGCAC
AviII        2245  TGCGCA               TGC^GCA
AvrII         752  CCTAGG               C^CTAGG
BbsI         2676  GAAGAC               GAAGAC(2/6)
BclI         2684  TGATCA               T^GATCA
BfrI         1491  CTTAAG               C^TTAAG
BlnI          752  CCTAGG               C^CTAGG
BpmI         2670  CTGGAG               CTGGAG(16/14)
BpuAI        2676  GAAGAC               GAAGAC(2/6)
BsaHI        2345  GACGCC               G[GA]^CG[CT]C
BsaI          257  GGTCTC               GGTCTC(1/5)
BsaWI         180  ACCGGA               [AT]^CCGG[AT]
BsmI         1654  GAATGC               GAATGC(1/-1)
BspDI          73  ATCGAT               AT^CGAT
BspLU11I     1276  ACATGT               A^CATGT
BspMI         774  GCAGGT               ACCTGC(4/8)
BstAPI       2547  GCATAATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1221  GGTGACC              G^GT[ACGT]ACC
BstPI        1221  GGTGACC              G^GT[ACGT]ACC
BstXI        1167  CCAGCTCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2493  CCTGAGG              CC^T[ACGT]AGG
ClaI           73  ATCGAT               AT^CGAT
DraII        2211  AGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       2358  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1837  CCATGG               C^C[GA][CT]GG
Eco81I       2493  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2625  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2211  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1221  GGTGACC              G^GT[ACGT]ACC
EcoRI         833  GAATTC               G^AATTC
EcoRV         405  GATATC               GAT^ATC
FauI          498  CCCGC                CCCGC(4/6)
FbaI         2684  TGATCA               T^GATCA
FspI         2245  TGCGCA               TGC^GCA
HaeII           5  AGCGCC               [GA]GCGC^[CT]
HgaI         2345  GACGC                GACGC(5/10)
Hin1I        2345  GACGCC               G[GA]^CG[CT]C
HindIII       974  AAGCTT               A^AGCTT
NcoI         1837  CCATGG               C^CATGG
PflMI        2357  CCACTTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2211  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I     2276  AACGTT               AA^CGTT
SexAI        1128  ACCAGGT              A^CC[AT]GGT
SnaBI         474  TACGTA               TAC^GTA
SphI         1233  GCATGC               GCATG^C
TfiI          120  GAATC                G^A[AT]TC
Van91I       2357  CCACTTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          168  TCTAGA               T^CTAGA
XcmI         1167  CCAGCTCAGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII          30  CGCG                 CG^CG
              451  CGCG                
AcsI          833  GAATTC               [GA]^AATT[CT]
              920  GAATTT              
AflIII       1276  ACATGT               A^C[GA][CT]GT
             1665  ACGTGT              
AhdI         1664  GACGTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2324  GACACGGAGTC         
AlwNI        1809  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2170  CAGTGTCTG           
ApoI          833  GAATTC               [GA]^AATT[CT]
              920  GAATTT              
Asp700I       160  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1315  GAACCCTTTC          
AspEI        1664  GACGTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2324  GACACGGAGTC         
BanI          748  GGTGCC               G^G[CT][GA]CC
             1611  GGCACC              
BanII         493  GGGCTC               G[GA]GC[CT]^C
             1343  GAGCCC              
BsaAI         474  TACGTA               [CT]AC^GT[GA]
             2019  TACGTG              
Bsp1407I     1577  TGTACA               T^GTACA
             2907  TGTACA              
BsrGI        1577  TGTACA               T^GTACA
             2907  TGTACA              
BssSI          91  CTCGTG               CACGAG(-5/-1)
             1692  CTCGTG              
BstUI          30  CGCG                 CG^CG
              451  CGCG                
Eam1105I     1664  GACGTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2324  GACACGGAGTC         
EcoT14I       752  CCTAGG               C^C[AT][AT]GG
             1837  CCATGG              
HaeIII        156  GGCC                 GG^CC
             1798  GGCC                
HincII       1272  GTTGAC               GT[CT]^[GA]AC
             1405  GTTGAC              
HindII       1272  GTTGAC               GT[CT]^[GA]AC
             1405  GTTGAC              
MvnI           30  CGCG                 CG^CG
              451  CGCG                
PvuII         668  CAGCTG               CAG^CTG
             1809  CAGCTG              
SspBI        1577  TGTACA               T^GTACA
             2907  TGTACA              
SspI          865  AATATT               AAT^ATT
             2784  AATATT              
StyI          752  CCTAGG               C^C[AT][AT]GG
             1837  CCATGG              
XmnI          160  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1315  GAACCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AfaI        AluI        AseI        AsnI        
AspHI       AvaII       BbvI        BcnI        BfaI        
BmyI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRII      Fnu4HI      FokI        HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I