################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspI AvaI AviII AvrII BbeI BbvCI BcgI BciVI BclI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI StuI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 2431 GTAGAC GT^[AC][GT]AC AflII 2444 CTTAAG C^TTAAG AflIII 2199 ACATGT A^C[GA][CT]GT AhdI 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 383 GTGCAC G^TGCAC ApaLI 383 GTGCAC G^TGCAC AspEI 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 926 GAGCTC G[GA]GC[CT]^C BbrPI 386 CACGTG CAC^GTG BfrI 2444 CTTAAG C^TTAAG BglII 1443 AGATCT A^GATCT BsaAI 386 CACGTG [CT]AC^GT[GA] BspLU11I 2199 ACATGT A^CATGT BsrBI 477 CCGCTC CCGCTC(-3/-3) BsrDI 67 CATTGC GCAATG(2/0) BsrFI 111 GCCGGT [GA]^CCGG[CT] BsrI 1434 ACTGG ACTGG(1/-1) BstF5I 919 CATCC GGATG(2/0) Cfr10I 111 GCCGGT [GA]^CCGG[CT] DraIII 833 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1001 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 404 GATATC GAT^ATC FauI 105 CCCGC CCCGC(4/6) FokI 919 CATCC GGATG(9/13) HgaI 862 GACGC GACGC(5/10) MspA1I 106 CCGCTG C[AC]G^C[GT]G NheI 2342 GCTAGC G^CTAGC PmaCI 386 CACGTG CAC^GTG PmlI 386 CACGTG CAC^GTG SacI 926 GAGCTC GAGCT^C SpeI 2318 ACTAGT A^CTAGT SwaI 1942 ATTTAAAT ATTT^AAAT XcmI 237 CCACCAGCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AseI 1903 ATTAAT AT^TAAT 2133 ATTAAT AsnI 1903 ATTAAT AT^TAAT 2133 ATTAAT BalI 1507 TGGCCA TGG^CCA 1712 TGGCCA BamHI 161 GGATCC G^GATCC 1367 GGATCC BanI 1158 GGCACC G^G[CT][GA]CC 1403 GGTGCC BcnI 182 CCCGG CC^[GC]GG 1084 CCGGG BpmI 94 CTCCAG CTGGAG(16/14) 10 CTGGAG BsaI 519 GAGACC GGTCTC(1/5) 1099 GGTCTC BsmFI 686 GGGAC GGGAC(10/14) 121 GTCCC Bsp1407I 2010 TGTACA T^GTACA 2392 TGTACA BsrGI 2010 TGTACA T^GTACA 2392 TGTACA BstXI 240 CCAGCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 267 CCACAGTGGTGG CfoI 82 GCGC GCG^C 1154 GCGC DraI 1943 TTTAAA TTT^AAA 2370 TTTAAA DsaI 768 CCATGG C^C[GA][CT]GG 2236 CCATGG EcoRI 251 GAATTC G^AATTC 1340 GAATTC EcoT14I 768 CCATGG C^C[AT][AT]GG 2236 CCATGG HhaI 82 GCGC GCG^C 1154 GCGC HinP1I 82 GCGC G^CGC 1154 GCGC HindIII 26 AAGCTT A^AGCTT 1575 AAGCTT MluNI 1507 TGGCCA TGG^CCA 1712 TGGCCA MscI 1507 TGGCCA TGG^CCA 1712 TGGCCA NciI 182 CCCGG CC^[GC]GG 1084 CCGGG NcoI 768 CCATGG C^CATGG 2236 CCATGG NspI 1482 ACATGC [GA]CATG^[CT] 2199 ACATGT PshBI 1903 ATTAAT AT^TAAT 2133 ATTAAT SapI 36 GAAGAGC GCTCTTC(1/4) 564 GCTCTTC SfaNI 279 GCATC GCATC(5/9) 499 GCATC SmlI 1972 CTCAAG C^T[CT][GA]AG 2444 CTTAAG SspBI 2010 TGTACA T^GTACA 2392 TGTACA StyI 768 CCATGG C^C[AT][AT]GG 2236 CCATGG TaqI 255 TCGA T^CGA 717 TCGA TthHB8I 255 TCGA T^CGA 717 TCGA XbaI 703 TCTAGA T^CTAGA 968 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII