##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AlwNI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspI AvaI
AviII AvrII BbeI BbvCI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspMI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
StuI Tth111I Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2431 GTAGAC GT^[AC][GT]AC
AflII 2444 CTTAAG C^TTAAG
AflIII 2199 ACATGT A^C[GA][CT]GT
AhdI 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 383 GTGCAC G^TGCAC
ApaLI 383 GTGCAC G^TGCAC
AspEI 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 926 GAGCTC G[GA]GC[CT]^C
BbrPI 386 CACGTG CAC^GTG
BfrI 2444 CTTAAG C^TTAAG
BglII 1443 AGATCT A^GATCT
BsaAI 386 CACGTG [CT]AC^GT[GA]
BspLU11I 2199 ACATGT A^CATGT
BsrBI 477 CCGCTC CCGCTC(-3/-3)
BsrDI 67 CATTGC GCAATG(2/0)
BsrFI 111 GCCGGT [GA]^CCGG[CT]
BsrI 1434 ACTGG ACTGG(1/-1)
BstF5I 919 CATCC GGATG(2/0)
Cfr10I 111 GCCGGT [GA]^CCGG[CT]
DraIII 833 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1389 GACGGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1001 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 404 GATATC GAT^ATC
FauI 105 CCCGC CCCGC(4/6)
FokI 919 CATCC GGATG(9/13)
HgaI 862 GACGC GACGC(5/10)
MspA1I 106 CCGCTG C[AC]G^C[GT]G
NheI 2342 GCTAGC G^CTAGC
PmaCI 386 CACGTG CAC^GTG
PmlI 386 CACGTG CAC^GTG
SacI 926 GAGCTC GAGCT^C
SpeI 2318 ACTAGT A^CTAGT
SwaI 1942 ATTTAAAT ATTT^AAAT
XcmI 237 CCACCAGCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AseI 1903 ATTAAT AT^TAAT
2133 ATTAAT
AsnI 1903 ATTAAT AT^TAAT
2133 ATTAAT
BalI 1507 TGGCCA TGG^CCA
1712 TGGCCA
BamHI 161 GGATCC G^GATCC
1367 GGATCC
BanI 1158 GGCACC G^G[CT][GA]CC
1403 GGTGCC
BcnI 182 CCCGG CC^[GC]GG
1084 CCGGG
BpmI 94 CTCCAG CTGGAG(16/14)
10 CTGGAG
BsaI 519 GAGACC GGTCTC(1/5)
1099 GGTCTC
BsmFI 686 GGGAC GGGAC(10/14)
121 GTCCC
Bsp1407I 2010 TGTACA T^GTACA
2392 TGTACA
BsrGI 2010 TGTACA T^GTACA
2392 TGTACA
BstXI 240 CCAGCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
267 CCACAGTGGTGG
CfoI 82 GCGC GCG^C
1154 GCGC
DraI 1943 TTTAAA TTT^AAA
2370 TTTAAA
DsaI 768 CCATGG C^C[GA][CT]GG
2236 CCATGG
EcoRI 251 GAATTC G^AATTC
1340 GAATTC
EcoT14I 768 CCATGG C^C[AT][AT]GG
2236 CCATGG
HhaI 82 GCGC GCG^C
1154 GCGC
HinP1I 82 GCGC G^CGC
1154 GCGC
HindIII 26 AAGCTT A^AGCTT
1575 AAGCTT
MluNI 1507 TGGCCA TGG^CCA
1712 TGGCCA
MscI 1507 TGGCCA TGG^CCA
1712 TGGCCA
NciI 182 CCCGG CC^[GC]GG
1084 CCGGG
NcoI 768 CCATGG C^CATGG
2236 CCATGG
NspI 1482 ACATGC [GA]CATG^[CT]
2199 ACATGT
PshBI 1903 ATTAAT AT^TAAT
2133 ATTAAT
SapI 36 GAAGAGC GCTCTTC(1/4)
564 GCTCTTC
SfaNI 279 GCATC GCATC(5/9)
499 GCATC
SmlI 1972 CTCAAG C^T[CT][GA]AG
2444 CTTAAG
SspBI 2010 TGTACA T^GTACA
2392 TGTACA
StyI 768 CCATGG C^C[AT][AT]GG
2236 CCATGG
TaqI 255 TCGA T^CGA
717 TCGA
TthHB8I 255 TCGA T^CGA
717 TCGA
XbaI 703 TCTAGA T^CTAGA
968 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII