##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BciVI BclI BfrI BglI BlnI
BsaBI BsaHI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I ClaI CpoI
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI Tth111I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 2698 AAATTC [GA]^AATT[CT]
AflIII 1739 ACATGT A^C[GA][CT]GT
AlwI 552 GATCC GGATC(4/5)
AocI 2472 CCTCAGG CC^T[ACGT]AGG
ApoI 2698 AAATTC [GA]^AATT[CT]
AvaI 2410 CTCGAG C^[CT]CG[GA]G
BbrPI 1074 CACGTG CAC^GTG
BglII 1669 AGATCT A^GATCT
BsaAI 1074 CACGTG [CT]AC^GT[GA]
BsaI 70 GAGACC GGTCTC(1/5)
BsmI 475 GAATGC GAATGC(1/-1)
BsoBI 2410 CTCGAG C^[CT]CG[GA]G
BspLU11I 1739 ACATGT A^CATGT
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BsrDI 2465 GCAATG GCAATG(2/0)
Bsu36I 2472 CCTCAGG CC^T[ACGT]AGG
EagI 1064 CGGCCG C^GGCCG
EclXI 1064 CGGCCG C^GGCCG
Eco52I 1064 CGGCCG C^GGCCG
Eco81I 2472 CCTCAGG CC^T[ACGT]AGG
HindIII 2074 AAGCTT A^AGCTT
NcoI 1627 CCATGG C^CATGG
NspI 1739 ACATGT [GA]CATG^[CT]
PaeR7I 2410 CTCGAG C^TCGAG
PflMI 2123 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1074 CACGTG CAC^GTG
PmlI 1074 CACGTG CAC^GTG
PshAI 1481 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 531 CCTGCAGG CCTGCA^GG
Sse8387I 531 CCTGCAGG CCTGCA^GG
SspI 1867 AATATT AAT^ATT
StuI 420 AGGCCT AGG^CCT
Van91I 2123 CCAGTTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2034 TCTAGA T^CTAGA
XhoI 2410 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
ApaI 1069 GGGCCC GGGCC^C
1234 GGGCCC
BbvCI 991 CCTCAGC CCTCAGC(-5/-2)
83 GCTGAGG
BcnI 1067 CCGGG CC^[GC]GG
1788 CCCGG
BlpI 1039 GCTCAGC GC^T[ACGT]AGC
2211 GCTGAGC
Bpu1102I 1039 GCTCAGC GC^T[ACGT]AGC
2211 GCTGAGC
BsiEI 1064 CGGCCG CG[GA][CT]^CG
2348 CGGTCG
BsmBI 656 CGTCTC CGTCTC(1/5)
63 GAGACG
BsmFI 1978 GGGAC GGGAC(10/14)
282 GTCCC
BspMI 374 ACCTGC ACCTGC(4/8)
530 ACCTGC
BsrFI 1048 ACCGGC [GA]^CCGG[CT]
1797 GCCGGT
BstAPI 15 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1965 GCAGGGCCTGC
BstYI 551 AGATCC [GA]^GATC[CT]
1669 AGATCT
CelII 1039 GCTCAGC GC^T[ACGT]AGC
2211 GCTGAGC
CfoI 1028 GCGC GCG^C
2528 GCGC
Cfr10I 1048 ACCGGC [GA]^CCGG[CT]
1797 GCCGGT
DpnII 552 GATC ^GATC
1670 GATC
DraI 1814 TTTAAA TTT^AAA
2694 TTTAAA
DraIII 2192 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
2537 CACTCTGTG
EaeI 1064 CGGCCG [CT]^GGCC[GA]
1190 TGGCCG
EcoT14I 1627 CCATGG C^C[AT][AT]GG
1878 CCAAGG
FauI 1168 CCCGC CCCGC(4/6)
2343 GCGGG
HhaI 1028 GCGC GCG^C
2528 GCGC
HinP1I 1028 GCGC G^CGC
2528 GCGC
HincII 1478 GTTGAC GT[CT]^[GA]AC
1843 GTCAAC
HindII 1478 GTTGAC GT[CT]^[GA]AC
1843 GTCAAC
MaeII 66 ACGT A^CGT
1075 ACGT
MboI 552 GATC ^GATC
1670 GATC
MflI 551 AGATCC [GA]^GATC[CT]
1669 AGATCT
NciI 1067 CCGGG CC^[GC]GG
1788 CCCGG
NdeII 552 GATC ^GATC
1670 GATC
PstI 532 CTGCAG CTGCA^G
1013 CTGCAG
SapI 759 GAAGAGC GCTCTTC(1/4)
582 GCTCTTC
Sau3AI 552 GATC ^GATC
1670 GATC
StyI 1627 CCATGG C^C[AT][AT]GG
1878 CCAAGG
TfiI 2512 GATTC G^A[AT]TC
2663 GAATC
Tsp509I 2046 AATT ^AATT
2699 AATT
XcmI 555 CCAGTACCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2281 CCACATCTCCGCTGG
XhoII 551 AGATCC [GA]^GATC[CT]
1669 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanI BanII BbsI BbvI
BcgI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DraII DsaI EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau96I ScrFI SfaNI
SfcI SmlI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I