##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BbrPI BbsI BcgI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 1875 GAATTT [GA]^AATT[CT]
AcyI 625 GGCGCC G[GA]^CG[CT]C
AflIII 2137 ACGTGT A^C[GA][CT]GT
ApaI 1432 GGGCCC GGGCC^C
ApoI 1875 GAATTT [GA]^AATT[CT]
BalI 171 TGGCCA TGG^CCA
BamHI 852 GGATCC G^GATCC
BbeI 625 GGCGCC GGCGC^C
BbvCI 65 GCTGAGG CCTCAGC(-5/-2)
BciVI 99 GGATAC GTATCC(6/5)
BsaHI 625 GGCGCC G[GA]^CG[CT]C
BsaI 1733 GGTCTC GGTCTC(1/5)
BseRI 439 CTCCTC GAGGAG(10/8)
BsgI 1382 CTGCAC GTGCAG(16/14)
BsmI 418 GAATGC GAATGC(1/-1)
BspMI 184 GCAGGT ACCTGC(4/8)
BsrDI 1101 GCAATG GCAATG(2/0)
BssSI 2012 CACGAG CACGAG(-5/-1)
CfoI 626 GCGC GCG^C
FauI 118 GCGGG CCCGC(4/6)
HaeII 625 GGCGCC [GA]GCGC^[CT]
HhaI 626 GCGC GCG^C
Hin1I 625 GGCGCC G[GA]^CG[CT]C
HinP1I 626 GCGC G^CGC
KasI 625 GGCGCC G^GCGCC
MluNI 171 TGGCCA TGG^CCA
MscI 171 TGGCCA TGG^CCA
MspA1I 782 CAGCTG C[AC]G^C[GT]G
NarI 625 GGCGCC GG^CGCC
NspI 526 GCATGT [GA]CATG^[CT]
PflMI 998 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 782 CAGCTG CAG^CTG
SfoI 625 GGCGCC GGC^GCC
StuI 1260 AGGCCT AGG^CCT
TaqI 206 TCGA T^CGA
TthHB8I 206 TCGA T^CGA
Van91I 998 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 734 TCTAGA T^CTAGA
XcmI 191 CCACTGGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 1903 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2067 GACTGCCTGTC
AlwNI 668 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1053 CAGTGTCTG
AspEI 1903 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2067 GACTGCCTGTC
AspHI 676 GAGCAC G[AT]GC[AT]^C
2034 GTGCTC
BcnI 1322 CCGGG CC^[GC]GG
1614 CCCGG
BlpI 343 GCTAAGC GC^T[ACGT]AGC
1916 GCTTAGC
Bpu1102I 343 GCTAAGC GC^T[ACGT]AGC
1916 GCTTAGC
BsaWI 213 ACCGGA [AT]^CCGG[AT]
614 TCCGGT
BsiHKAI 676 GAGCAC G[AT]GC[AT]^C
2034 GTGCTC
BsrFI 15 GCCGGT [GA]^CCGG[CT]
947 ACCGGC
BstAPI 181 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
803 GCATCTCCTGC
BstXI 125 CCAGTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1096 CCACAGCAATGG
CelII 343 GCTAAGC GC^T[ACGT]AGC
1916 GCTTAGC
Cfr10I 15 GCCGGT [GA]^CCGG[CT]
947 ACCGGC
EaeI 171 TGGCCA [CT]^GGCC[GA]
234 TGGCCG
Eam1105I 1903 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2067 GACTGCCTGTC
HindIII 506 AAGCTT A^AGCTT
1829 AAGCTT
NciI 1322 CCGGG CC^[GC]GG
1614 CCCGG
NheI 515 GCTAGC G^CTAGC
1295 GCTAGC
PstI 29 CTGCAG CTGCA^G
720 CTGCAG
SmlI 683 CTCAAG C^T[CT][GA]AG
1862 CTTGAG
TfiI 217 GAATC G^A[AT]TC
934 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII