##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp700I AspI
BbrPI BbvCI BcgI BclI BfrI
BsaAI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1060 GGTACC G^GTACC
AccI 452 GTAGAC GT^[AC][GT]AC
AccIII 76 TCCGGA T^CCGGA
AhdI 2272 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 91 GTGCAC G^TGCAC
AocI 2053 CCTCAGG CC^T[ACGT]AGG
ApaI 1424 GGGCCC GGGCC^C
ApaLI 91 GTGCAC G^TGCAC
Asp718I 1060 GGTACC G^GTACC
AspEI 2272 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 91 GTGCAC G[AT]GC[AT]^C
AviII 2330 TGCGCA TGC^GCA
BbeI 1148 GGCGCC GGCGC^C
BciVI 574 GTATCC GTATCC(6/5)
BglI 2040 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 76 TCCGGA [AT]^CCGG[AT]
BseAI 76 TCCGGA T^CCGGA
BsiEI 184 CGACCG CG[GA][CT]^CG
BsiHKAI 91 GTGCAC G[AT]GC[AT]^C
BspEI 76 TCCGGA T^CCGGA
BsrDI 2470 GCAATG GCAATG(2/0)
BsrFI 499 GCCGGC [GA]^CCGG[CT]
BssSI 164 CACGAG CACGAG(-5/-1)
BstAPI 2022 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2053 CCTCAGG CC^T[ACGT]AGG
Cfr10I 499 GCCGGC [GA]^CCGG[CT]
Eam1105I 2272 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2053 CCTCAGG CC^T[ACGT]AGG
FauI 1817 CCCGC CCCGC(4/6)
FspI 2330 TGCGCA TGC^GCA
KasI 1148 GGCGCC G^GCGCC
KpnI 1060 GGTACC GGTAC^C
KspI 216 CCGCGG CCGC^GG
MroI 76 TCCGGA T^CCGGA
MslI 2282 CATCTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 499 GCCGGC GCC^GGC
NarI 1148 GGCGCC GG^CGCC
NgoMIV 499 GCCGGC G^CCGGC
PflMI 1125 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 216 CCGCGG CCGC^GG
SapI 334 GCTCTTC GCTCTTC(1/4)
SbfI 875 CCTGCAGG CCTGCA^GG
SfoI 1148 GGCGCC GGC^GCC
SmaI 65 CCCGGG CCC^GGG
Sse8387I 875 CCTGCAGG CCTGCA^GG
Van91I 1125 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1087 CCATGCTCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 1359 AAATTT [GA]^AATT[CT]
2296 AAATTC
AcyI 1148 GGCGCC G[GA]^CG[CT]C
2048 GGCGTC
AflIII 448 ACATGT A^C[GA][CT]GT
458 ACATGT
ApoI 1359 AAATTT [GA]^AATT[CT]
2296 AAATTC
BamHI 543 GGATCC G^GATCC
1769 GGATCC
BlpI 590 GCTGAGC GC^T[ACGT]AGC
1256 GCTGAGC
Bpu1102I 590 GCTGAGC GC^T[ACGT]AGC
1256 GCTGAGC
BsaHI 1148 GGCGCC G[GA]^CG[CT]C
2048 GGCGTC
BseRI 1317 CTCCTC GAGGAG(10/8)
675 GAGGAG
BsgI 1200 CTGCAC GTGCAG(16/14)
30 GTGCAG
BspLU11I 448 ACATGT A^CATGT
458 ACATGT
BsrBI 364 CCGCTC CCGCTC(-3/-3)
663 GAGCGG
BstXI 1490 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2268 CCAGGACTCTGG
CelII 590 GCTGAGC GC^T[ACGT]AGC
1256 GCTGAGC
HaeII 679 AGCGCC [GA]GCGC^[CT]
1148 GGCGCC
Hin1I 1148 GGCGCC G[GA]^CG[CT]C
2048 GGCGTC
PaeR7I 375 CTCGAG C^TCGAG
999 CTCGAG
PleI 2272 GACTC GAGTC(4/5)
1899 GAGTC
StuI 1529 AGGCCT AGG^CCT
1626 AGGCCT
TfiI 1106 GAATC G^A[AT]TC
2422 GAATC
Tsp45I 1648 GTGAC ^GT[GC]AC
2025 GTGAC
XhoI 375 CTCGAG C^TCGAG
999 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII AvrII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlnI BmyI BpmI
BpuAI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp509I TspRI TthHB8I XhoII