################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Alw44I ApaLI AscI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BciVI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI ClaI CpoI DrdI EcoNI EcoRV EcoT22I FspI HindIII KasI KpnI MamI MluI MroI NarI NcoI NsiI PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccI 2087 GTATAC GT^[AC][GT]AC AhdI 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1487 CCTCAGG CC^T[ACGT]AGG Aor51HI 1347 AGCGCT AGC^GCT AseI 2002 ATTAAT AT^TAAT AsnI 2002 ATTAAT AT^TAAT AspEI 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 89 CACGTG CAC^GTG BbvCI 1547 GCTGAGG CCTCAGC(-5/-2) BclI 683 TGATCA T^GATCA BlpI 99 GCTGAGC GC^T[ACGT]AGC Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC BsaAI 89 CACGTG [CT]AC^GT[GA] BseRI 161 CTCCTC GAGGAG(10/8) BsiWI 1265 CGTACG C^GTACG BsrBI 241 CCGCTC CCGCTC(-3/-3) BssSI 1044 CACGAG CACGAG(-5/-1) Bst1107I 2087 GTATAC GTA^TAC BstBI 756 TTCGAA TT^CGAA BstEII 2012 GGTTACC G^GT[ACGT]ACC BstPI 2012 GGTTACC G^GT[ACGT]ACC BstZ17I 2087 GTATAC GTA^TAC Bsu36I 1487 CCTCAGG CC^T[ACGT]AGG CelII 99 GCTGAGC GC^T[ACGT]AGC DraI 2171 TTTAAA TTT^AAA DraIII 852 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1347 AGCGCT AGC^GCT Eco81I 1487 CCTCAGG CC^T[ACGT]AGG EcoO65I 2012 GGTTACC G^GT[ACGT]ACC EcoRI 1640 GAATTC G^AATTC EcoT14I 1330 CCTTGG C^C[AT][AT]GG FbaI 683 TGATCA T^GATCA FseI 256 GGCCGGCC GGCCGG^CC HincII 1918 GTTAAC GT[CT]^[GA]AC HindII 1918 GTTAAC GT[CT]^[GA]AC HpaI 1918 GTTAAC GTT^AAC MfeI 1955 CAATTG C^AATTG MunI 1955 CAATTG C^AATTG NaeI 257 GCCGGC GCC^GGC NdeI 2120 CATATG CA^TATG NgoMIV 257 GCCGGC G^CCGGC NheI 576 GCTAGC G^CTAGC NotI 9 GCGGCCGC GC^GGCCGC NruI 1202 TCGCGA TCG^CGA NspV 756 TTCGAA TT^CGAA PacI 1999 TTAATTAA TTAAT^TAA PaeR7I 1932 CTCGAG C^TCGAG PmaCI 89 CACGTG CAC^GTG PmlI 89 CACGTG CAC^GTG PshBI 2002 ATTAAT AT^TAAT PstI 1911 CTGCAG CTGCA^G ScaI 1837 AGTACT AGT^ACT SfiI 251 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 756 TTCGAA TT^CGAA SmaI 22 CCCGGG CCC^GGG StuI 1007 AGGCCT AGG^CCT StyI 1330 CCTTGG C^C[AT][AT]GG TfiI 1760 GAATC G^A[AT]TC XhoI 1932 CTCGAG C^TCGAG XmaCI 22 CCCGGG C^CCGGG XmaI 22 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 1640 GAATTC [GA]^AATT[CT] 1803 GAATTT AlwNI 121 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1125 CAGGGCCTG ApaI 406 GGGCCC GGGCC^C 1326 GGGCCC ApoI 1640 GAATTC [GA]^AATT[CT] 1803 GAATTT AvaI 22 CCCGGG C^[CT]CG[GA]G 1932 CTCGAG BanI 1283 GGCACC G^G[CT][GA]CC 1443 GGTGCC BbsI 1722 GAAGAC GAAGAC(2/6) 1613 GTCTTC BcgI 385 CGAAGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 941 CGAGTGTTCTGC BpmI 1113 CTGGAG CTGGAG(16/14) 2234 CTGGAG BpuAI 1722 GAAGAC GAAGAC(2/6) 1613 GTCTTC BsmAI 898 GAGAC GTCTC(1/5) 1133 GTCTC BsoBI 22 CCCGGG C^[CT]CG[GA]G 1932 CTCGAG BspMI 718 ACCTGC ACCTGC(4/8) 995 ACCTGC BstAPI 133 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1303 GCAGTCGTTGC BstYI 447 GGATCT [GA]^GATC[CT] 1493 GGATCT MaeII 90 ACGT A^CGT 856 ACGT MflI 447 GGATCT [GA]^GATC[CT] 1493 GGATCT SphI 549 GCATGC GCATG^C 1260 GCATGC XhoII 447 GGATCT [GA]^GATC[CT] 1493 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I