##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BciVI BfrI BglII
BlnI BsaBI BsaI BsaWI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI ClaI
CpoI DrdI EcoNI EcoRV EcoT22I
FspI HindIII KasI KpnI MamI
MluI MroI NarI NcoI NsiI
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 2087 GTATAC GT^[AC][GT]AC
AhdI 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1487 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1347 AGCGCT AGC^GCT
AseI 2002 ATTAAT AT^TAAT
AsnI 2002 ATTAAT AT^TAAT
AspEI 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 89 CACGTG CAC^GTG
BbvCI 1547 GCTGAGG CCTCAGC(-5/-2)
BclI 683 TGATCA T^GATCA
BlpI 99 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC
BsaAI 89 CACGTG [CT]AC^GT[GA]
BseRI 161 CTCCTC GAGGAG(10/8)
BsiWI 1265 CGTACG C^GTACG
BsrBI 241 CCGCTC CCGCTC(-3/-3)
BssSI 1044 CACGAG CACGAG(-5/-1)
Bst1107I 2087 GTATAC GTA^TAC
BstBI 756 TTCGAA TT^CGAA
BstEII 2012 GGTTACC G^GT[ACGT]ACC
BstPI 2012 GGTTACC G^GT[ACGT]ACC
BstZ17I 2087 GTATAC GTA^TAC
Bsu36I 1487 CCTCAGG CC^T[ACGT]AGG
CelII 99 GCTGAGC GC^T[ACGT]AGC
DraI 2171 TTTAAA TTT^AAA
DraIII 852 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 331 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1347 AGCGCT AGC^GCT
Eco81I 1487 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2012 GGTTACC G^GT[ACGT]ACC
EcoRI 1640 GAATTC G^AATTC
EcoT14I 1330 CCTTGG C^C[AT][AT]GG
FbaI 683 TGATCA T^GATCA
FseI 256 GGCCGGCC GGCCGG^CC
HincII 1918 GTTAAC GT[CT]^[GA]AC
HindII 1918 GTTAAC GT[CT]^[GA]AC
HpaI 1918 GTTAAC GTT^AAC
MfeI 1955 CAATTG C^AATTG
MunI 1955 CAATTG C^AATTG
NaeI 257 GCCGGC GCC^GGC
NdeI 2120 CATATG CA^TATG
NgoMIV 257 GCCGGC G^CCGGC
NheI 576 GCTAGC G^CTAGC
NotI 9 GCGGCCGC GC^GGCCGC
NruI 1202 TCGCGA TCG^CGA
NspV 756 TTCGAA TT^CGAA
PacI 1999 TTAATTAA TTAAT^TAA
PaeR7I 1932 CTCGAG C^TCGAG
PmaCI 89 CACGTG CAC^GTG
PmlI 89 CACGTG CAC^GTG
PshBI 2002 ATTAAT AT^TAAT
PstI 1911 CTGCAG CTGCA^G
ScaI 1837 AGTACT AGT^ACT
SfiI 251 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 756 TTCGAA TT^CGAA
SmaI 22 CCCGGG CCC^GGG
StuI 1007 AGGCCT AGG^CCT
StyI 1330 CCTTGG C^C[AT][AT]GG
TfiI 1760 GAATC G^A[AT]TC
XhoI 1932 CTCGAG C^TCGAG
XmaCI 22 CCCGGG C^CCGGG
XmaI 22 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1640 GAATTC [GA]^AATT[CT]
1803 GAATTT
AlwNI 121 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1125 CAGGGCCTG
ApaI 406 GGGCCC GGGCC^C
1326 GGGCCC
ApoI 1640 GAATTC [GA]^AATT[CT]
1803 GAATTT
AvaI 22 CCCGGG C^[CT]CG[GA]G
1932 CTCGAG
BanI 1283 GGCACC G^G[CT][GA]CC
1443 GGTGCC
BbsI 1722 GAAGAC GAAGAC(2/6)
1613 GTCTTC
BcgI 385 CGAAGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
941 CGAGTGTTCTGC
BpmI 1113 CTGGAG CTGGAG(16/14)
2234 CTGGAG
BpuAI 1722 GAAGAC GAAGAC(2/6)
1613 GTCTTC
BsmAI 898 GAGAC GTCTC(1/5)
1133 GTCTC
BsoBI 22 CCCGGG C^[CT]CG[GA]G
1932 CTCGAG
BspMI 718 ACCTGC ACCTGC(4/8)
995 ACCTGC
BstAPI 133 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1303 GCAGTCGTTGC
BstYI 447 GGATCT [GA]^GATC[CT]
1493 GGATCT
MaeII 90 ACGT A^CGT
856 ACGT
MflI 447 GGATCT [GA]^GATC[CT]
1493 GGATCT
SphI 549 GCATGC GCATG^C
1260 GCATGC
XhoII 447 GGATCT [GA]^GATC[CT]
1493 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BalI BanII
BbvI BcnI BfaI BglI BmyI
BsaHI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I