##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII BalI BamHI BbeI BbrPI
BciVI BglI BlpI Bpu1102I BsaAI
BsaHI BseAI BseRI BsiWI BsoBI
Bsp1407I BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FseI
FspI HgaI Hin1I HpaI KasI
KpnI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 2656 GTAGAC GT^[AC][GT]AC
AfaI 1176 GTAC GT^AC
AflII 2081 CTTAAG C^TTAAG
AflIII 2126 ACATGT A^C[GA][CT]GT
Alw44I 1017 GTGCAC G^TGCAC
AlwNI 134 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1017 GTGCAC G^TGCAC
AseI 2647 ATTAAT AT^TAAT
AsnI 2647 ATTAAT AT^TAAT
Asp700I 342 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1167 CCTAGG C^CTAGG
BanI 2389 GGTGCC G^G[CT][GA]CC
BbvCI 867 GCTGAGG CCTCAGC(-5/-2)
BcgI 143 CGAGCTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2081 CTTAAG C^TTAAG
BglII 689 AGATCT A^GATCT
BlnI 1167 CCTAGG C^CTAGG
BsaBI 690 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 509 ACCGGA [AT]^CCGG[AT]
BsiEI 262 CGGTCG CG[GA][CT]^CG
BsmI 633 GCATTC GAATGC(1/-1)
BspDI 1890 ATCGAT AT^CGAT
BspLU11I 2126 ACATGT A^CATGT
BsrBI 155 CCGCTC CCGCTC(-3/-3)
ClaI 1890 ATCGAT AT^CGAT
DraIII 1184 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 322 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 251 TGGCCG [CT]^GGCC[GA]
EcoRI 781 GAATTC G^AATTC
EcoT22I 2893 ATGCAT ATGCA^T
HaeII 37 AGCGCC [GA]GCGC^[CT]
HincII 1252 GTTGAC GT[CT]^[GA]AC
HindII 1252 GTTGAC GT[CT]^[GA]AC
HindIII 410 AAGCTT A^AGCTT
KspI 236 CCGCGG CCGC^GG
MaeII 711 ACGT A^CGT
MamI 690 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1224 CCATGG C^CATGG
NsiI 2893 ATGCAT ATGCA^T
PacI 2644 TTAATTAA TTAAT^TAA
PflMI 613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 222 GACTC GAGTC(4/5)
PpuMI 428 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2647 ATTAAT AT^TAAT
PstI 768 CTGCAG CTGCA^G
PvuII 230 CAGCTG CAG^CTG
RsaI 1176 GTAC GT^AC
SacII 236 CCGCGG CCGC^GG
SpeI 2698 ACTAGT A^CTAGT
StuI 871 AGGCCT AGG^CCT
TaqI 1891 TCGA T^CGA
TthHB8I 1891 TCGA T^CGA
Van91I 613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 975 CCAGTCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 342 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 429 GGACC G^G[AT]CC
972 GGACC
BanII 144 GAGCTC G[GA]GC[CT]^C
1126 GAGCTC
BbsI 852 GAAGAC GAAGAC(2/6)
2439 GAAGAC
BclI 405 TGATCA T^GATCA
1477 TGATCA
BpmI 91 CTCCAG CTGGAG(16/14)
1328 CTGGAG
BpuAI 852 GAAGAC GAAGAC(2/6)
2439 GAAGAC
BsaI 2374 GAGACC GGTCTC(1/5)
565 GGTCTC
BsmBI 69 GAGACG CGTCTC(1/5)
353 GAGACG
BsmFI 722 GTCCC GGGAC(10/14)
2269 GTCCC
BspHI 828 TCATGA T^CATGA
1900 TCATGA
BspMI 580 ACCTGC ACCTGC(4/8)
962 ACCTGC
BstAPI 584 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1300 GCAGATTGTGC
BstYI 417 AGATCC [GA]^GATC[CT]
689 AGATCT
DraII 428 AGGACCC [GA]G^G[ACGT]CC[CT]
821 AGGCCCT
DsaI 236 CCGCGG C^C[GA][CT]GG
1224 CCATGG
EarI 642 CTCTTC CTCTTC(1/4)
2579 GAAGAG
EcoO109I 428 AGGACCC [GA]G^G[ACGT]CC[CT]
821 AGGCCCT
EcoRV 1065 GATATC GAT^ATC
1323 GATATC
FauI 154 CCCGC CCCGC(4/6)
15 GCGGG
FbaI 405 TGATCA T^GATCA
1477 TGATCA
Ksp632I 642 CTCTTC CTCTTC(1/4)
2579 GAAGAG
MflI 417 AGATCC [GA]^GATC[CT]
689 AGATCT
MslI 829 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1225 CATGGCAGTG
NlaIV 429 GGACCC GG[ACGT]^[ACGT]CC
2389 GGTGCC
RcaI 828 TCATGA T^CATGA
1900 TCATGA
SacI 144 GAGCTC GAGCT^C
1126 GAGCTC
SmlI 172 CTCAAG C^T[CT][GA]AG
2081 CTTAAG
SwaI 2107 ATTTAAAT ATTT^AAAT
2485 ATTTAAAT
XhoII 417 AGATCC [GA]^GATC[CT]
689 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AspHI BbvI BcnI BfaI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NspI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI