################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII BalI BamHI BbeI BbrPI BciVI BglI BlpI Bpu1102I BsaAI BsaHI BseAI BseRI BsiWI BsoBI Bsp1407I BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FseI FspI HgaI Hin1I HpaI KasI KpnI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 2656 GTAGAC GT^[AC][GT]AC AfaI 1176 GTAC GT^AC AflII 2081 CTTAAG C^TTAAG AflIII 2126 ACATGT A^C[GA][CT]GT Alw44I 1017 GTGCAC G^TGCAC AlwNI 134 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1017 GTGCAC G^TGCAC AseI 2647 ATTAAT AT^TAAT AsnI 2647 ATTAAT AT^TAAT Asp700I 342 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 1167 CCTAGG C^CTAGG BanI 2389 GGTGCC G^G[CT][GA]CC BbvCI 867 GCTGAGG CCTCAGC(-5/-2) BcgI 143 CGAGCTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2081 CTTAAG C^TTAAG BglII 689 AGATCT A^GATCT BlnI 1167 CCTAGG C^CTAGG BsaBI 690 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 509 ACCGGA [AT]^CCGG[AT] BsiEI 262 CGGTCG CG[GA][CT]^CG BsmI 633 GCATTC GAATGC(1/-1) BspDI 1890 ATCGAT AT^CGAT BspLU11I 2126 ACATGT A^CATGT BsrBI 155 CCGCTC CCGCTC(-3/-3) ClaI 1890 ATCGAT AT^CGAT DraIII 1184 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 322 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 251 TGGCCG [CT]^GGCC[GA] EcoRI 781 GAATTC G^AATTC EcoT22I 2893 ATGCAT ATGCA^T HaeII 37 AGCGCC [GA]GCGC^[CT] HincII 1252 GTTGAC GT[CT]^[GA]AC HindII 1252 GTTGAC GT[CT]^[GA]AC HindIII 410 AAGCTT A^AGCTT KspI 236 CCGCGG CCGC^GG MaeII 711 ACGT A^CGT MamI 690 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1224 CCATGG C^CATGG NsiI 2893 ATGCAT ATGCA^T PacI 2644 TTAATTAA TTAAT^TAA PflMI 613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 222 GACTC GAGTC(4/5) PpuMI 428 AGGACCC [GA]G^G[AT]CC[CT] PshBI 2647 ATTAAT AT^TAAT PstI 768 CTGCAG CTGCA^G PvuII 230 CAGCTG CAG^CTG RsaI 1176 GTAC GT^AC SacII 236 CCGCGG CCGC^GG SpeI 2698 ACTAGT A^CTAGT StuI 871 AGGCCT AGG^CCT TaqI 1891 TCGA T^CGA TthHB8I 1891 TCGA T^CGA Van91I 613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 975 CCAGTCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 342 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AvaII 429 GGACC G^G[AT]CC 972 GGACC BanII 144 GAGCTC G[GA]GC[CT]^C 1126 GAGCTC BbsI 852 GAAGAC GAAGAC(2/6) 2439 GAAGAC BclI 405 TGATCA T^GATCA 1477 TGATCA BpmI 91 CTCCAG CTGGAG(16/14) 1328 CTGGAG BpuAI 852 GAAGAC GAAGAC(2/6) 2439 GAAGAC BsaI 2374 GAGACC GGTCTC(1/5) 565 GGTCTC BsmBI 69 GAGACG CGTCTC(1/5) 353 GAGACG BsmFI 722 GTCCC GGGAC(10/14) 2269 GTCCC BspHI 828 TCATGA T^CATGA 1900 TCATGA BspMI 580 ACCTGC ACCTGC(4/8) 962 ACCTGC BstAPI 584 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1300 GCAGATTGTGC BstYI 417 AGATCC [GA]^GATC[CT] 689 AGATCT DraII 428 AGGACCC [GA]G^G[ACGT]CC[CT] 821 AGGCCCT DsaI 236 CCGCGG C^C[GA][CT]GG 1224 CCATGG EarI 642 CTCTTC CTCTTC(1/4) 2579 GAAGAG EcoO109I 428 AGGACCC [GA]G^G[ACGT]CC[CT] 821 AGGCCCT EcoRV 1065 GATATC GAT^ATC 1323 GATATC FauI 154 CCCGC CCCGC(4/6) 15 GCGGG FbaI 405 TGATCA T^GATCA 1477 TGATCA Ksp632I 642 CTCTTC CTCTTC(1/4) 2579 GAAGAG MflI 417 AGATCC [GA]^GATC[CT] 689 AGATCT MslI 829 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1225 CATGGCAGTG NlaIV 429 GGACCC GG[ACGT]^[ACGT]CC 2389 GGTGCC RcaI 828 TCATGA T^CATGA 1900 TCATGA SacI 144 GAGCTC GAGCT^C 1126 GAGCTC SmlI 172 CTCAAG C^T[CT][GA]AG 2081 CTTAAG SwaI 2107 ATTTAAAT ATTT^AAAT 2485 ATTTAAAT XhoII 417 AGATCC [GA]^GATC[CT] 689 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AspHI BbvI BcnI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NspI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI