##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI AscI
Asp718I AspEI AspI AviII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BpuAI
BsaWI BseAI BsiWI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CpoI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI FauI FbaI FseI
FspI HindIII HpaI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfiI SgrAI SmlI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1689 GTCGAC GT^[AC][GT]AC
Alw44I 2598 GTGCAC G^TGCAC
AlwNI 15 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2598 GTGCAC G^TGCAC
AseI 2838 ATTAAT AT^TAAT
AsnI 2838 ATTAAT AT^TAAT
Asp700I 364 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 587 GGACC G^G[AT]CC
BanI 100 GGCGCC G^G[CT][GA]CC
BbeI 100 GGCGCC GGCGC^C
BglII 1547 AGATCT A^GATCT
BlpI 1092 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1092 GCTCAGC GC^T[ACGT]AGC
BsaAI 219 CACGTA [CT]AC^GT[GA]
BsgI 1623 CTGCAC GTGCAG(16/14)
BsmBI 926 GAGACG CGTCTC(1/5)
BspDI 737 ATCGAT AT^CGAT
BspLU11I 1709 ACATGT A^CATGT
BspMI 945 GCAGGT ACCTGC(4/8)
BsrDI 2966 CATTGC GCAATG(2/0)
BsrFI 97 GCCGGC [GA]^CCGG[CT]
BstBI 1856 TTCGAA TT^CGAA
BstYI 1547 AGATCT [GA]^GATC[CT]
CelII 1092 GCTCAGC GC^T[ACGT]AGC
Cfr10I 97 GCCGGC [GA]^CCGG[CT]
ClaI 737 ATCGAT AT^CGAT
DraIII 2285 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 968 GACCGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 161 CGGCCG C^GGCCG
EclXI 161 CGGCCG C^GGCCG
Eco52I 161 CGGCCG C^GGCCG
EcoRV 1890 GATATC GAT^ATC
EcoT22I 2870 ATGCAT ATGCA^T
HincII 1689 GTCGAC GT[CT]^[GA]AC
HindII 1689 GTCGAC GT[CT]^[GA]AC
KasI 100 GGCGCC G^GCGCC
MflI 1547 AGATCT [GA]^GATC[CT]
NaeI 97 GCCGGC GCC^GGC
NarI 100 GGCGCC GG^CGCC
NgoMIV 97 GCCGGC G^CCGGC
NotI 160 GCGGCCGC GC^GGCCGC
NsiI 2870 ATGCAT ATGCA^T
NspI 1709 ACATGT [GA]CATG^[CT]
NspV 1856 TTCGAA TT^CGAA
PleI 87 GAGTC GAGTC(4/5)
PshAI 1614 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2838 ATTAAT AT^TAAT
PstI 1457 CTGCAG CTGCA^G
PvuII 2077 CAGCTG CAG^CTG
SacI 3057 GAGCTC GAGCT^C
SalI 1689 GTCGAC G^TCGAC
SfoI 100 GGCGCC GGC^GCC
SfuI 1856 TTCGAA TT^CGAA
SpeI 646 ACTAGT A^CTAGT
XbaI 1279 TCTAGA T^CTAGA
XhoII 1547 AGATCT [GA]^GATC[CT]
XmnI 364 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 100 GGCGCC G[GA]^CG[CT]C
1686 GGCGTC
AflIII 218 ACACGT A^C[GA][CT]GT
1709 ACATGT
ApaI 29 GGGCCC GGGCC^C
265 GGGCCC
AvaI 26 CCCGGG C^[CT]CG[GA]G
191 CCCGGG
AvrII 921 CCTAGG C^CTAGG
2051 CCTAGG
BlnI 921 CCTAGG C^CTAGG
2051 CCTAGG
BpmI 2074 CTCCAG CTGGAG(16/14)
3054 CTGGAG
BsaHI 100 GGCGCC G[GA]^CG[CT]C
1686 GGCGTC
BsaI 917 GAGACC GGTCTC(1/5)
2006 GGTCTC
BsiEI 161 CGGCCG CG[GA][CT]^CG
1691 CGACCG
BsmI 1207 GCATTC GAATGC(1/-1)
1240 GCATTC
BsoBI 26 CCCGGG C^[CT]CG[GA]G
191 CCCGGG
DraII 29 GGGCCCC [GA]G^G[ACGT]CC[CT]
265 GGGCCCT
EaeI 161 CGGCCG [CT]^GGCC[GA]
1307 TGGCCG
EcoO109I 29 GGGCCCC [GA]G^G[ACGT]CC[CT]
265 GGGCCCT
HaeII 100 GGCGCC [GA]GCGC^[CT]
142 GGCGCT
HgaI 38 GCGTC GACGC(5/10)
1687 GCGTC
Hin1I 100 GGCGCC G[GA]^CG[CT]C
1686 GGCGTC
SfcI 1457 CTGCAG C^T[GA][CT]AG
2121 CTACAG
SmaI 26 CCCGGG CCC^GGG
191 CCCGGG
XmaCI 26 CCCGGG C^CCGGG
191 CCCGGG
XmaI 26 CCCGGG C^CCGGG
191 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI BanII BbvI
BcnI BfaI BmyI BsaBI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspHI BsrGI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I