##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AgeI AhdI ApaI ApoI
AscI AspEI AspI BamHI BanII
BbeI BbvCI BciVI BclI BfrI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PflFI PflMI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1822 GGTACC G^GTACC
AcyI 1371 GACGCC G[GA]^CG[CT]C
AocI 1817 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1396 AGCGCT AGC^GCT
Asp700I 1058 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1822 GGTACC G^GTACC
AviII 769 TGCGCA TGC^GCA
AvrII 1500 CCTAGG C^CTAGG
BbrPI 1409 CACGTG CAC^GTG
BcgI 1559 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 729 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1660 AGATCT A^GATCT
BlnI 1500 CCTAGG C^CTAGG
BlpI 753 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 753 GCTGAGC GC^T[ACGT]AGC
BsaHI 1371 GACGCC G[GA]^CG[CT]C
BsiEI 1476 CGGTCG CG[GA][CT]^CG
BsmI 542 GAATGC GAATGC(1/-1)
BsrBI 759 CCGCTC CCGCTC(-3/-3)
BsrFI 1474 GCCGGT [GA]^CCGG[CT]
BssSI 1827 CACGAG CACGAG(-5/-1)
BstAPI 636 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 2118 GGATG GGATG(2/0)
Bsu36I 1817 CCTGAGG CC^T[ACGT]AGG
CelII 753 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1474 GCCGGT [GA]^CCGG[CT]
DraIII 1332 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1396 AGCGCT AGC^GCT
Eco81I 1817 CCTGAGG CC^T[ACGT]AGG
FokI 2118 GGATG GGATG(9/13)
FspI 769 TGCGCA TGC^GCA
HaeII 1396 AGCGCT [GA]GCGC^[CT]
Hin1I 1371 GACGCC G[GA]^CG[CT]C
HincII 858 GTTGAC GT[CT]^[GA]AC
HindII 858 GTTGAC GT[CT]^[GA]AC
HindIII 598 AAGCTT A^AGCTT
KpnI 1822 GGTACC GGTAC^C
MslI 45 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PacI 2401 TTAATTAA TTAAT^TAA
PaeR7I 762 CTCGAG C^TCGAG
PmaCI 1409 CACGTG CAC^GTG
PmlI 1409 CACGTG CAC^GTG
PpuMI 1678 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1061 GCTCTTC GCTCTTC(1/4)
SexAI 1092 ACCAGGT A^CC[AT]GGT
SphI 641 GCATGC GCATG^C
StuI 1831 AGGCCT AGG^CCT
XhoI 762 CTCGAG C^TCGAG
XmnI 1058 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1368 GTAGAC GT^[AC][GT]AC
1592 GTCTAC
AflIII 862 ACATGT A^C[GA][CT]GT
1921 ACATGT
Alw44I 523 GTGCAC G^TGCAC
2373 GTGCAC
ApaLI 523 GTGCAC G^TGCAC
2373 GTGCAC
AseI 113 ATTAAT AT^TAAT
2449 ATTAAT
AsnI 113 ATTAAT AT^TAAT
2449 ATTAAT
AspHI 523 GTGCAC G[AT]GC[AT]^C
2373 GTGCAC
AvaI 762 CTCGAG C^[CT]CG[GA]G
1891 CTCGGG
BpmI 936 CTGGAG CTGGAG(16/14)
1179 CTGGAG
BsaAI 878 TACGTG [CT]AC^GT[GA]
1409 CACGTG
BsgI 1406 CTGCAC GTGCAG(16/14)
2022 CTGCAC
BsiHKAI 523 GTGCAC G[AT]GC[AT]^C
2373 GTGCAC
BsoBI 762 CTCGAG C^[CT]CG[GA]G
1891 CTCGGG
BspLU11I 862 ACATGT A^CATGT
1921 ACATGT
BstXI 811 CCAAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1459 CCAAAGACTTGG
DraI 261 TTTAAA TTT^AAA
1448 TTTAAA
DrdI 397 GACTAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
419 GACTTCTCCGTC
DsaI 1549 CCATGG C^C[GA][CT]GG
1732 CCATGG
FauI 2067 CCCGC CCCGC(4/6)
607 GCGGG
NcoI 1549 CCATGG C^CATGG
1732 CCATGG
PshBI 113 ATTAAT AT^TAAT
2449 ATTAAT
PstI 634 CTGCAG CTGCA^G
1026 CTGCAG
SmlI 762 CTCGAG C^T[CT][GA]AG
2126 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII