################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AgeI AhdI ApaI ApoI AscI AspEI AspI BamHI BanII BbeI BbvCI BciVI BclI BfrI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 1822 GGTACC G^GTACC AcyI 1371 GACGCC G[GA]^CG[CT]C AocI 1817 CCTGAGG CC^T[ACGT]AGG Aor51HI 1396 AGCGCT AGC^GCT Asp700I 1058 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1822 GGTACC G^GTACC AviII 769 TGCGCA TGC^GCA AvrII 1500 CCTAGG C^CTAGG BbrPI 1409 CACGTG CAC^GTG BcgI 1559 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 729 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1660 AGATCT A^GATCT BlnI 1500 CCTAGG C^CTAGG BlpI 753 GCTGAGC GC^T[ACGT]AGC Bpu1102I 753 GCTGAGC GC^T[ACGT]AGC BsaHI 1371 GACGCC G[GA]^CG[CT]C BsiEI 1476 CGGTCG CG[GA][CT]^CG BsmI 542 GAATGC GAATGC(1/-1) BsrBI 759 CCGCTC CCGCTC(-3/-3) BsrFI 1474 GCCGGT [GA]^CCGG[CT] BssSI 1827 CACGAG CACGAG(-5/-1) BstAPI 636 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 2118 GGATG GGATG(2/0) Bsu36I 1817 CCTGAGG CC^T[ACGT]AGG CelII 753 GCTGAGC GC^T[ACGT]AGC Cfr10I 1474 GCCGGT [GA]^CCGG[CT] DraIII 1332 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1396 AGCGCT AGC^GCT Eco81I 1817 CCTGAGG CC^T[ACGT]AGG FokI 2118 GGATG GGATG(9/13) FspI 769 TGCGCA TGC^GCA HaeII 1396 AGCGCT [GA]GCGC^[CT] Hin1I 1371 GACGCC G[GA]^CG[CT]C HincII 858 GTTGAC GT[CT]^[GA]AC HindII 858 GTTGAC GT[CT]^[GA]AC HindIII 598 AAGCTT A^AGCTT KpnI 1822 GGTACC GGTAC^C MslI 45 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PacI 2401 TTAATTAA TTAAT^TAA PaeR7I 762 CTCGAG C^TCGAG PmaCI 1409 CACGTG CAC^GTG PmlI 1409 CACGTG CAC^GTG PpuMI 1678 AGGACCT [GA]G^G[AT]CC[CT] SapI 1061 GCTCTTC GCTCTTC(1/4) SexAI 1092 ACCAGGT A^CC[AT]GGT SphI 641 GCATGC GCATG^C StuI 1831 AGGCCT AGG^CCT XhoI 762 CTCGAG C^TCGAG XmnI 1058 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1368 GTAGAC GT^[AC][GT]AC 1592 GTCTAC AflIII 862 ACATGT A^C[GA][CT]GT 1921 ACATGT Alw44I 523 GTGCAC G^TGCAC 2373 GTGCAC ApaLI 523 GTGCAC G^TGCAC 2373 GTGCAC AseI 113 ATTAAT AT^TAAT 2449 ATTAAT AsnI 113 ATTAAT AT^TAAT 2449 ATTAAT AspHI 523 GTGCAC G[AT]GC[AT]^C 2373 GTGCAC AvaI 762 CTCGAG C^[CT]CG[GA]G 1891 CTCGGG BpmI 936 CTGGAG CTGGAG(16/14) 1179 CTGGAG BsaAI 878 TACGTG [CT]AC^GT[GA] 1409 CACGTG BsgI 1406 CTGCAC GTGCAG(16/14) 2022 CTGCAC BsiHKAI 523 GTGCAC G[AT]GC[AT]^C 2373 GTGCAC BsoBI 762 CTCGAG C^[CT]CG[GA]G 1891 CTCGGG BspLU11I 862 ACATGT A^CATGT 1921 ACATGT BstXI 811 CCAAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1459 CCAAAGACTTGG DraI 261 TTTAAA TTT^AAA 1448 TTTAAA DrdI 397 GACTAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 419 GACTTCTCCGTC DsaI 1549 CCATGG C^C[GA][CT]GG 1732 CCATGG FauI 2067 CCCGC CCCGC(4/6) 607 GCGGG NcoI 1549 CCATGG C^CATGG 1732 CCATGG PshBI 113 ATTAAT AT^TAAT 2449 ATTAAT PstI 634 CTGCAG CTGCA^G 1026 CTGCAG SmlI 762 CTCGAG C^T[CT][GA]AG 2126 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BalI BanI BbsI BbvI BcnI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII