##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        AscI        AseI        AsnI        
Asp718I     AspEI       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BfrI        
BglI        BlnI        BsaAI       BsaBI       BseAI       
BsiWI       BspDI       BspEI       BspHI       BsrBI       
BsrFI       BssHII      Bst1107I    BstAPI      BstBI       
BstZ17I     Cfr10I      ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoT22I     
FseI        HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SphI        Sse8387I    SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1992  GTAGAC               GT^[AC][GT]AC
Alw44I       2215  GTGCAC               G^TGCAC
Aor51HI       570  AGCGCT               AGC^GCT
ApaLI        2215  GTGCAC               G^TGCAC
Asp700I      2040  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          350  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          284  CCCGGG               C^[CT]CG[GA]G
AviII         921  TGCGCA               TGC^GCA
BalI         1586  TGGCCA               TGG^CCA
BclI           21  TGATCA               T^GATCA
BglII        1202  AGATCT               A^GATCT
BsaWI        1932  TCCGGT               [AT]^CCGG[AT]
BsiEI        1934  CGGTCG               CG[GA][CT]^CG
BsoBI         284  CCCGGG               C^[CT]CG[GA]G
BssSI         178  CTCGTG               CACGAG(-5/-1)
BstEII       2839  GGTAACC              G^GT[ACGT]ACC
BstPI        2839  GGTAACC              G^GT[ACGT]ACC
BstXI        1425  CCAAGAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI         1586  TGGCCA               [CT]^GGCC[GA]
Eco47III      570  AGCGCT               AGC^GCT
EcoO65I      2839  GGTAACC              G^GT[ACGT]ACC
EcoRV        2482  GATATC               GAT^ATC
FbaI           21  TGATCA               T^GATCA
FspI          921  TGCGCA               TGC^GCA
HindIII      1724  AAGCTT               A^AGCTT
KspI         2113  CCGCGG               CCGC^GG
MluNI        1586  TGGCCA               TGG^CCA
MscI         1586  TGGCCA               TGG^CCA
PflFI         350  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         478  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        2500  GGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2186  GACTGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2005  CTGCAG               CTGCA^G
PvuII        1122  CAGCTG               CAG^CTG
SacI          331  GAGCTC               GAGCT^C
SacII        2113  CCGCGG               CCGC^GG
SapI         2243  GCTCTTC              GCTCTTC(1/4)
ScaI          604  AGTACT               AGT^ACT
SexAI        1431  ACCTGGT              A^CC[AT]GGT
SmaI          284  CCCGGG               CCC^GGG
SpeI          817  ACTAGT               A^CTAGT
StuI         1674  AGGCCT               AGG^CCT
Tth111I       350  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        478  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          474  CCATCCATTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         284  CCCGGG               C^CCGGG
XmaI          284  CCCGGG               C^CCGGG
XmnI         2040  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII        2114  CGCG                 CG^CG
             2240  CGCG                
AcyI          203  GACGCC               G[GA]^CG[CT]C
             1061  GACGCC              
AlwNI          94  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2089  CAGAAACTG           
ApaI          106  GGGCCC               GGGCC^C
              400  GGGCCC              
BbvCI        2850  CCTCAGC              CCTCAGC(-5/-2)
              392  GCTGAGG             
BsaHI         203  GACGCC               G[GA]^CG[CT]C
             1061  GACGCC              
BsaI         1435  GGTCTC               GGTCTC(1/5)
             1637  GGTCTC              
BsmBI         577  CGTCTC               CGTCTC(1/5)
             2192  CGTCTC              
BsmI         1762  GCATTC               GAATGC(1/-1)
             2145  GCATTC              
BsrDI          58  CATTGC               GCAATG(2/0)
             3000  GCAATG              
BstUI        2114  CGCG                 CG^CG
             2240  CGCG                
BstYI        1202  AGATCT               [GA]^GATC[CT]
             2348  GGATCT              
Cac8I          86  GCTTGC               GC[ACGT]^[ACGT]GC
              255  GCCTGC              
DraI         1234  TTTAAA               TTT^AAA
             3035  TTTAAA              
DraIII       1073  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2953  CACCCTGTG           
EcoNI         997  CCTCTGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2904  CCTGGTTTAGG         
HaeII         570  AGCGCT               [GA]GCGC^[CT]
             2278  GGCGCT              
HgaI          203  GACGC                GACGC(5/10)
             1061  GACGC               
Hin1I         203  GACGCC               G[GA]^CG[CT]C
             1061  GACGCC              
MaeII         542  ACGT                 A^CGT
              840  ACGT                
MflI         1202  AGATCT               [GA]^GATC[CT]
             2348  GGATCT              
MvnI         2114  CGCG                 CG^CG
             2240  CGCG                
SfcI           77  CTACAG               C^T[GA][CT]AG
             2005  CTGCAG              
XhoII        1202  AGATCT               [GA]^GATC[CT]
             2348  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AocI        ApoI        AspHI       AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    Bsp1407I    
BspLU11I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       Bsu36I      CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        Eco81I      EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SspBI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I