################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BsaAI BsaBI BseAI BsiWI BspDI BspEI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoT22I FseI HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1992 GTAGAC GT^[AC][GT]AC Alw44I 2215 GTGCAC G^TGCAC Aor51HI 570 AGCGCT AGC^GCT ApaLI 2215 GTGCAC G^TGCAC Asp700I 2040 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 284 CCCGGG C^[CT]CG[GA]G AviII 921 TGCGCA TGC^GCA BalI 1586 TGGCCA TGG^CCA BclI 21 TGATCA T^GATCA BglII 1202 AGATCT A^GATCT BsaWI 1932 TCCGGT [AT]^CCGG[AT] BsiEI 1934 CGGTCG CG[GA][CT]^CG BsoBI 284 CCCGGG C^[CT]CG[GA]G BssSI 178 CTCGTG CACGAG(-5/-1) BstEII 2839 GGTAACC G^GT[ACGT]ACC BstPI 2839 GGTAACC G^GT[ACGT]ACC BstXI 1425 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 1586 TGGCCA [CT]^GGCC[GA] Eco47III 570 AGCGCT AGC^GCT EcoO65I 2839 GGTAACC G^GT[ACGT]ACC EcoRV 2482 GATATC GAT^ATC FbaI 21 TGATCA T^GATCA FspI 921 TGCGCA TGC^GCA HindIII 1724 AAGCTT A^AGCTT KspI 2113 CCGCGG CCGC^GG MluNI 1586 TGGCCA TGG^CCA MscI 1586 TGGCCA TGG^CCA PflFI 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 478 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2500 GGGACCT [GA]G^G[AT]CC[CT] PshAI 2186 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2005 CTGCAG CTGCA^G PvuII 1122 CAGCTG CAG^CTG SacI 331 GAGCTC GAGCT^C SacII 2113 CCGCGG CCGC^GG SapI 2243 GCTCTTC GCTCTTC(1/4) ScaI 604 AGTACT AGT^ACT SexAI 1431 ACCTGGT A^CC[AT]GGT SmaI 284 CCCGGG CCC^GGG SpeI 817 ACTAGT A^CTAGT StuI 1674 AGGCCT AGG^CCT Tth111I 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 478 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 474 CCATCCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 284 CCCGGG C^CCGGG XmaI 284 CCCGGG C^CCGGG XmnI 2040 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 2114 CGCG CG^CG 2240 CGCG AcyI 203 GACGCC G[GA]^CG[CT]C 1061 GACGCC AlwNI 94 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG 2089 CAGAAACTG ApaI 106 GGGCCC GGGCC^C 400 GGGCCC BbvCI 2850 CCTCAGC CCTCAGC(-5/-2) 392 GCTGAGG BsaHI 203 GACGCC G[GA]^CG[CT]C 1061 GACGCC BsaI 1435 GGTCTC GGTCTC(1/5) 1637 GGTCTC BsmBI 577 CGTCTC CGTCTC(1/5) 2192 CGTCTC BsmI 1762 GCATTC GAATGC(1/-1) 2145 GCATTC BsrDI 58 CATTGC GCAATG(2/0) 3000 GCAATG BstUI 2114 CGCG CG^CG 2240 CGCG BstYI 1202 AGATCT [GA]^GATC[CT] 2348 GGATCT Cac8I 86 GCTTGC GC[ACGT]^[ACGT]GC 255 GCCTGC DraI 1234 TTTAAA TTT^AAA 3035 TTTAAA DraIII 1073 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 2953 CACCCTGTG EcoNI 997 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2904 CCTGGTTTAGG HaeII 570 AGCGCT [GA]GCGC^[CT] 2278 GGCGCT HgaI 203 GACGC GACGC(5/10) 1061 GACGC Hin1I 203 GACGCC G[GA]^CG[CT]C 1061 GACGCC MaeII 542 ACGT A^CGT 840 ACGT MflI 1202 AGATCT [GA]^GATC[CT] 2348 GGATCT MvnI 2114 CGCG CG^CG 2240 CGCG SfcI 77 CTACAG C^T[GA][CT]AG 2005 CTGCAG XhoII 1202 AGATCT [GA]^GATC[CT] 2348 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI Bsu36I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I