##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BseAI
BsiWI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1992 GTAGAC GT^[AC][GT]AC
Alw44I 2215 GTGCAC G^TGCAC
Aor51HI 570 AGCGCT AGC^GCT
ApaLI 2215 GTGCAC G^TGCAC
Asp700I 2040 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 284 CCCGGG C^[CT]CG[GA]G
AviII 921 TGCGCA TGC^GCA
BalI 1586 TGGCCA TGG^CCA
BclI 21 TGATCA T^GATCA
BglII 1202 AGATCT A^GATCT
BsaWI 1932 TCCGGT [AT]^CCGG[AT]
BsiEI 1934 CGGTCG CG[GA][CT]^CG
BsoBI 284 CCCGGG C^[CT]CG[GA]G
BssSI 178 CTCGTG CACGAG(-5/-1)
BstEII 2839 GGTAACC G^GT[ACGT]ACC
BstPI 2839 GGTAACC G^GT[ACGT]ACC
BstXI 1425 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 1586 TGGCCA [CT]^GGCC[GA]
Eco47III 570 AGCGCT AGC^GCT
EcoO65I 2839 GGTAACC G^GT[ACGT]ACC
EcoRV 2482 GATATC GAT^ATC
FbaI 21 TGATCA T^GATCA
FspI 921 TGCGCA TGC^GCA
HindIII 1724 AAGCTT A^AGCTT
KspI 2113 CCGCGG CCGC^GG
MluNI 1586 TGGCCA TGG^CCA
MscI 1586 TGGCCA TGG^CCA
PflFI 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 478 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2500 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2186 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2005 CTGCAG CTGCA^G
PvuII 1122 CAGCTG CAG^CTG
SacI 331 GAGCTC GAGCT^C
SacII 2113 CCGCGG CCGC^GG
SapI 2243 GCTCTTC GCTCTTC(1/4)
ScaI 604 AGTACT AGT^ACT
SexAI 1431 ACCTGGT A^CC[AT]GGT
SmaI 284 CCCGGG CCC^GGG
SpeI 817 ACTAGT A^CTAGT
StuI 1674 AGGCCT AGG^CCT
Tth111I 350 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 478 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 474 CCATCCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 284 CCCGGG C^CCGGG
XmaI 284 CCCGGG C^CCGGG
XmnI 2040 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2114 CGCG CG^CG
2240 CGCG
AcyI 203 GACGCC G[GA]^CG[CT]C
1061 GACGCC
AlwNI 94 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
2089 CAGAAACTG
ApaI 106 GGGCCC GGGCC^C
400 GGGCCC
BbvCI 2850 CCTCAGC CCTCAGC(-5/-2)
392 GCTGAGG
BsaHI 203 GACGCC G[GA]^CG[CT]C
1061 GACGCC
BsaI 1435 GGTCTC GGTCTC(1/5)
1637 GGTCTC
BsmBI 577 CGTCTC CGTCTC(1/5)
2192 CGTCTC
BsmI 1762 GCATTC GAATGC(1/-1)
2145 GCATTC
BsrDI 58 CATTGC GCAATG(2/0)
3000 GCAATG
BstUI 2114 CGCG CG^CG
2240 CGCG
BstYI 1202 AGATCT [GA]^GATC[CT]
2348 GGATCT
Cac8I 86 GCTTGC GC[ACGT]^[ACGT]GC
255 GCCTGC
DraI 1234 TTTAAA TTT^AAA
3035 TTTAAA
DraIII 1073 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2953 CACCCTGTG
EcoNI 997 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2904 CCTGGTTTAGG
HaeII 570 AGCGCT [GA]GCGC^[CT]
2278 GGCGCT
HgaI 203 GACGC GACGC(5/10)
1061 GACGC
Hin1I 203 GACGCC G[GA]^CG[CT]C
1061 GACGCC
MaeII 542 ACGT A^CGT
840 ACGT
MflI 1202 AGATCT [GA]^GATC[CT]
2348 GGATCT
MvnI 2114 CGCG CG^CG
2240 CGCG
SfcI 77 CTACAG C^T[GA][CT]AG
2005 CTGCAG
XhoII 1202 AGATCT [GA]^GATC[CT]
2348 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspLU11I BspMI BsrGI BsrI BssKI
BstF5I BstNI Bsu36I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I