################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccI AclI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI AspI AviII AvrII BbeI BbrPI BbvCI BfrI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BspDI BspHI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1167 GGTACC G^GTACC AccIII 600 TCCGGA T^CCGGA AcyI 2638 GACGCC G[GA]^CG[CT]C AflIII 754 ACATGT A^C[GA][CT]GT AhdI 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 752 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1167 GGTACC G^GTACC AspEI 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 1167 GGTACC G^G[CT][GA]CC BciVI 1671 GGATAC GTATCC(6/5) BfaI 1377 CTAG C^TAG BglII 1366 AGATCT A^GATCT BsaBI 2625 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2638 GACGCC G[GA]^CG[CT]C BseAI 600 TCCGGA T^CCGGA BseRI 2137 CTCCTC GAGGAG(10/8) BsmBI 256 CGTCTC CGTCTC(1/5) BsmI 1456 GAATGC GAATGC(1/-1) Bsp1407I 1991 TGTACA T^GTACA BspEI 600 TCCGGA T^CCGGA BspLU11I 754 ACATGT A^CATGT BsrBI 299 CCGCTC CCGCTC(-3/-3) BsrFI 11 ACCGGC [GA]^CCGG[CT] BsrGI 1991 TGTACA T^GTACA BssSI 287 CACGAG CACGAG(-5/-1) BstAPI 1063 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 969 CCAACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 11 ACCGGC [GA]^CCGG[CT] DraIII 739 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1033 GACTGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Hin1I 2638 GACGCC G[GA]^CG[CT]C KpnI 1167 GGTACC GGTAC^C MaeI 1377 CTAG C^TAG MamI 2625 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 600 TCCGGA T^CCGGA NcoI 2367 CCATGG C^CATGG PaeR7I 1601 CTCGAG C^TCGAG PpuMI 2065 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1424 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 551 GAAGAGC GCTCTTC(1/4) SexAI 951 ACCTGGT A^CC[AT]GGT SspBI 1991 TGTACA T^GTACA XcmI 2721 CCATAGATGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1601 CTCGAG C^TCGAG XmnI 752 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 245 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1766 CAGCTGCTG AvaI 1601 CTCGAG C^[CT]CG[GA]G 2241 CCCGAG BalI 1287 TGGCCA TGG^CCA 2370 TGGCCA BamHI 460 GGATCC G^GATCC 1550 GGATCC BcgI 789 CGATTCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 140 GCAGAGGTGTCG BclI 1402 TGATCA T^GATCA 2390 TGATCA BcnI 1249 CCGGG CC^[GC]GG 1731 CCGGG BglI 1072 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2306 GCCAGCCTGGC BpmI 710 CTCCAG CTGGAG(16/14) 1544 CTGGAG BsgI 2683 CTGCAC GTGCAG(16/14) 1440 GTGCAG BsoBI 1601 CTCGAG C^[CT]CG[GA]G 2241 CCCGAG BsrDI 1815 CATTGC GCAATG(2/0) 47 GCAATG BstBI 515 TTCGAA TT^CGAA 2452 TTCGAA DraI 320 TTTAAA TTT^AAA 2703 TTTAAA DraII 1920 GGGGCCT [GA]G^G[ACGT]CC[CT] 2065 AGGACCC EcoO109I 1920 GGGGCCT [GA]G^G[ACGT]CC[CT] 2065 AGGACCC FbaI 1402 TGATCA T^GATCA 2390 TGATCA MaeII 221 ACGT A^CGT 527 ACGT MluNI 1287 TGGCCA TGG^CCA 2370 TGGCCA MscI 1287 TGGCCA TGG^CCA 2370 TGGCCA NciI 1249 CCGGG CC^[GC]GG 1731 CCGGG NspV 515 TTCGAA TT^CGAA 2452 TTCGAA SfuI 515 TTCGAA TT^CGAA 2452 TTCGAA SmlI 274 CTTGAG C^T[CT][GA]AG 1601 CTCGAG SspI 722 AATATT AAT^ATT 1382 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcsI AfaI AluI Alw44I AlwI ApaLI ApoI AspHI AvaII BanII BbsI BbvI BmyI BpuAI BsaI BsaJI BsaWI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII