##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI AspI AviII AvrII BbeI
BbrPI BbvCI BfrI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BspDI
BspHI BssHII Bst1107I BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1167 GGTACC G^GTACC
AccIII 600 TCCGGA T^CCGGA
AcyI 2638 GACGCC G[GA]^CG[CT]C
AflIII 754 ACATGT A^C[GA][CT]GT
AhdI 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 752 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1167 GGTACC G^GTACC
AspEI 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1167 GGTACC G^G[CT][GA]CC
BciVI 1671 GGATAC GTATCC(6/5)
BfaI 1377 CTAG C^TAG
BglII 1366 AGATCT A^GATCT
BsaBI 2625 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2638 GACGCC G[GA]^CG[CT]C
BseAI 600 TCCGGA T^CCGGA
BseRI 2137 CTCCTC GAGGAG(10/8)
BsmBI 256 CGTCTC CGTCTC(1/5)
BsmI 1456 GAATGC GAATGC(1/-1)
Bsp1407I 1991 TGTACA T^GTACA
BspEI 600 TCCGGA T^CCGGA
BspLU11I 754 ACATGT A^CATGT
BsrBI 299 CCGCTC CCGCTC(-3/-3)
BsrFI 11 ACCGGC [GA]^CCGG[CT]
BsrGI 1991 TGTACA T^GTACA
BssSI 287 CACGAG CACGAG(-5/-1)
BstAPI 1063 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 969 CCAACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 11 ACCGGC [GA]^CCGG[CT]
DraIII 739 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1033 GACTGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1050 GACCAGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 2638 GACGCC G[GA]^CG[CT]C
KpnI 1167 GGTACC GGTAC^C
MaeI 1377 CTAG C^TAG
MamI 2625 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 600 TCCGGA T^CCGGA
NcoI 2367 CCATGG C^CATGG
PaeR7I 1601 CTCGAG C^TCGAG
PpuMI 2065 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1424 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 551 GAAGAGC GCTCTTC(1/4)
SexAI 951 ACCTGGT A^CC[AT]GGT
SspBI 1991 TGTACA T^GTACA
XcmI 2721 CCATAGATGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1601 CTCGAG C^TCGAG
XmnI 752 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 245 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1766 CAGCTGCTG
AvaI 1601 CTCGAG C^[CT]CG[GA]G
2241 CCCGAG
BalI 1287 TGGCCA TGG^CCA
2370 TGGCCA
BamHI 460 GGATCC G^GATCC
1550 GGATCC
BcgI 789 CGATTCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
140 GCAGAGGTGTCG
BclI 1402 TGATCA T^GATCA
2390 TGATCA
BcnI 1249 CCGGG CC^[GC]GG
1731 CCGGG
BglI 1072 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2306 GCCAGCCTGGC
BpmI 710 CTCCAG CTGGAG(16/14)
1544 CTGGAG
BsgI 2683 CTGCAC GTGCAG(16/14)
1440 GTGCAG
BsoBI 1601 CTCGAG C^[CT]CG[GA]G
2241 CCCGAG
BsrDI 1815 CATTGC GCAATG(2/0)
47 GCAATG
BstBI 515 TTCGAA TT^CGAA
2452 TTCGAA
DraI 320 TTTAAA TTT^AAA
2703 TTTAAA
DraII 1920 GGGGCCT [GA]G^G[ACGT]CC[CT]
2065 AGGACCC
EcoO109I 1920 GGGGCCT [GA]G^G[ACGT]CC[CT]
2065 AGGACCC
FbaI 1402 TGATCA T^GATCA
2390 TGATCA
MaeII 221 ACGT A^CGT
527 ACGT
MluNI 1287 TGGCCA TGG^CCA
2370 TGGCCA
MscI 1287 TGGCCA TGG^CCA
2370 TGGCCA
NciI 1249 CCGGG CC^[GC]GG
1731 CCGGG
NspV 515 TTCGAA TT^CGAA
2452 TTCGAA
SfuI 515 TTCGAA TT^CGAA
2452 TTCGAA
SmlI 274 CTTGAG C^T[CT][GA]AG
1601 CTCGAG
SspI 722 AATATT AAT^ATT
1382 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaII BanII BbsI BbvI BmyI
BpuAI BsaI BsaJI BsaWI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII