##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BsaAI BsiEI
BsiWI BsmBI BspHI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MaeIII
MfeI MluI MunI MvnI NdeI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SfiI SgrAI SnaBI SpeI SphI
SwaI Tsp45I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 79 enzymes) #
###################################
AccIII 1200 TCCGGA T^CCGGA
AcyI 824 GGCGCC G[GA]^CG[CT]C
AflIII 942 ACATGT A^C[GA][CT]GT
AhdI 2219 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 314 GTGCAC G^TGCAC
AlwI 1246 GATCC GGATC(4/5)
AlwNI 254 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1363 GGGCCC GGGCC^C
ApaLI 314 GTGCAC G^TGCAC
Asp700I 1786 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2219 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 580 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 13 CCCGGG C^[CT]CG[GA]G
BalI 1902 TGGCCA TGG^CCA
BanI 824 GGCGCC G^G[CT][GA]CC
BanII 1363 GGGCCC G[GA]GC[CT]^C
BbeI 824 GGCGCC GGCGC^C
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BsaBI 1264 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 824 GGCGCC G[GA]^CG[CT]C
BsaI 33 GGTCTC GGTCTC(1/5)
BseAI 1200 TCCGGA T^CCGGA
BsmFI 1113 GGGAC GGGAC(10/14)
BsmI 377 GAATGC GAATGC(1/-1)
BsoBI 13 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1266 TGTACA T^GTACA
BspDI 1990 ATCGAT AT^CGAT
BspEI 1200 TCCGGA T^CCGGA
BspLU11I 942 ACATGT A^CATGT
BsrBI 393 GAGCGG CCGCTC(-3/-3)
BsrFI 25 GCCGGC [GA]^CCGG[CT]
BsrGI 1266 TGTACA T^GTACA
BstBI 1644 TTCGAA TT^CGAA
BstXI 174 CCACCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1018 GCTGAGC GC^T[ACGT]AGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
ClaI 1990 ATCGAT AT^CGAT
DraI 2012 TTTAAA TTT^AAA
DraII 57 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1834 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1623 CCATGG C^C[GA][CT]GG
Eam1105I 2219 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 49 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 57 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 29 GCGGG CCCGC(4/6)
Hin1I 824 GGCGCC G[GA]^CG[CT]C
HincII 767 GTCAAC GT[CT]^[GA]AC
HindII 767 GTCAAC GT[CT]^[GA]AC
HindIII 554 AAGCTT A^AGCTT
KasI 824 GGCGCC G^GCGCC
MaeII 167 ACGT A^CGT
MamI 1264 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1902 TGGCCA TGG^CCA
MroI 1200 TCCGGA T^CCGGA
MscI 1902 TGGCCA TGG^CCA
NaeI 25 GCCGGC GCC^GGC
NarI 824 GGCGCC GG^CGCC
NcoI 1623 CCATGG C^CATGG
NgoMIV 25 GCCGGC G^CCGGC
NheI 1765 GCTAGC G^CTAGC
NspV 1644 TTCGAA TT^CGAA
PflFI 580 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 662 CAGCTG CAG^CTG
SapI 1818 GCTCTTC GCTCTTC(1/4)
SbfI 409 CCTGCAGG CCTGCA^GG
ScaI 1377 AGTACT AGT^ACT
SfoI 824 GGCGCC GGC^GCC
SfuI 1644 TTCGAA TT^CGAA
SmaI 13 CCCGGG CCC^GGG
SmlI 1157 CTCAAG C^T[CT][GA]AG
Sse8387I 409 CCTGCAGG CCTGCA^GG
SspBI 1266 TGTACA T^GTACA
SspI 872 AATATT AAT^ATT
StuI 1417 AGGCCT AGG^CCT
Tth111I 580 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
XmnI 1786 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1462 GAATTT [GA]^AATT[CT]
2188 AAATTC
ApoI 1462 GAATTT [GA]^AATT[CT]
2188 AAATTC
BbsI 1094 GAAGAC GAAGAC(2/6)
283 GTCTTC
BbvCI 1075 CCTCAGC CCTCAGC(-5/-2)
2144 CCTCAGC
BcnI 13 CCCGG CC^[GC]GG
14 CCGGG
BglII 1673 AGATCT A^GATCT
1697 AGATCT
BpuAI 1094 GAAGAC GAAGAC(2/6)
283 GTCTTC
BsaWI 680 ACCGGA [AT]^CCGG[AT]
1200 TCCGGA
BspMI 21 ACCTGC ACCTGC(4/8)
1014 ACCTGC
BsrDI 2182 CATTGC GCAATG(2/0)
1358 GCAATG
BstYI 1673 AGATCT [GA]^GATC[CT]
1697 AGATCT
EaeI 3 CGGCCA [CT]^GGCC[GA]
1902 TGGCCA
HaeII 824 GGCGCC [GA]GCGC^[CT]
1212 GGCGCT
HgaI 580 GACGC GACGC(5/10)
708 GCGTC
MflI 1673 AGATCT [GA]^GATC[CT]
1697 AGATCT
MspA1I 7 CAGCGG C[AC]G^C[GT]G
662 CAGCTG
NciI 13 CCCGG CC^[GC]GG
14 CCGGG
SexAI 309 ACCTGGT A^CC[AT]GGT
1835 ACCTGGT
XhoII 1673 AGATCT [GA]^GATC[CT]
1697 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I