##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflIII AgeI AhdI Alw44I ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BclI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI MroI MvnI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1659 GACGTC GACGT^C
AccI 1679 GTAGAC GT^[AC][GT]AC
AocI 96 CCTGAGG CC^T[ACGT]AGG
ApaI 2621 GGGCCC GGGCC^C
AseI 1810 ATTAAT AT^TAAT
AsnI 1810 ATTAAT AT^TAAT
BalI 1574 TGGCCA TGG^CCA
BanI 689 GGCGCC G^G[CT][GA]CC
BbeI 689 GGCGCC GGCGC^C
BbsI 1717 GAAGAC GAAGAC(2/6)
BcgI 1535 CGAATGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 541 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2121 AGATCT A^GATCT
BpuAI 1717 GAAGAC GAAGAC(2/6)
BsmI 2171 GAATGC GAATGC(1/-1)
BspHI 2388 TCATGA T^CATGA
BsrDI 1643 CATTGC GCAATG(2/0)
Bsu36I 96 CCTGAGG CC^T[ACGT]AGG
DraI 1853 TTTAAA TTT^AAA
DraIII 2762 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 112 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 96 CCTGAGG CC^T[ACGT]AGG
HgaI 200 GCGTC GACGC(5/10)
KasI 689 GGCGCC G^GCGCC
MfeI 2606 CAATTG C^AATTG
MluNI 1574 TGGCCA TGG^CCA
MscI 1574 TGGCCA TGG^CCA
MunI 2606 CAATTG C^AATTG
NarI 689 GGCGCC GG^CGCC
NcoI 2180 CCATGG C^CATGG
NdeI 2110 CATATG CA^TATG
PflMI 2154 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1810 ATTAAT AT^TAAT
RcaI 2388 TCATGA T^CATGA
ScaI 2135 AGTACT AGT^ACT
SfoI 689 GGCGCC GGC^GCC
SmaI 2197 CCCGGG CCC^GGG
StuI 77 AGGCCT AGG^CCT
TaqI 1534 TCGA T^CGA
TthHB8I 1534 TCGA T^CGA
Van91I 2154 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2197 CCCGGG C^CCGGG
XmaI 2197 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 689 GGCGCC G[GA]^CG[CT]C
1659 GACGTC
AfaI 1566 GTAC GT^AC
2136 GTAC
AflII 1171 CTTAAG C^TTAAG
1313 CTTAAG
AlwNI 829 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
838 CAGGTTCTG
AspHI 742 GTGCTC G[AT]GC[AT]^C
1332 GAGCAC
BciVI 366 GGATAC GTATCC(6/5)
1344 GGATAC
BfrI 1171 CTTAAG C^TTAAG
1313 CTTAAG
BsaHI 689 GGCGCC G[GA]^CG[CT]C
1659 GACGTC
BsiHKAI 742 GTGCTC G[AT]GC[AT]^C
1332 GAGCAC
BssSI 1228 CACGAG CACGAG(-5/-1)
233 CTCGTG
BstAPI 828 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2143 GCATAGCTTGC
BstYI 2121 AGATCT [GA]^GATC[CT]
2295 AGATCC
EaeI 1574 TGGCCA [CT]^GGCC[GA]
1592 CGGCCA
FauI 432 CCCGC CCCGC(4/6)
2255 CCCGC
Hin1I 689 GGCGCC G[GA]^CG[CT]C
1659 GACGTC
MflI 2121 AGATCT [GA]^GATC[CT]
2295 AGATCC
MslI 280 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2386 CATCATGATG
PvuII 2245 CAGCTG CAG^CTG
2436 CAGCTG
RsaI 1566 GTAC GT^AC
2136 GTAC
SphI 41 GCATGC GCATG^C
279 GCATGC
XbaI 1455 TCTAGA T^CTAGA
1872 TCTAGA
XhoII 2121 AGATCT [GA]^GATC[CT]
2295 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AluI AlwI Aor51HI AvaI
AvaII BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI