################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflIII AgeI AhdI Alw44I ApaLI ApoI AscI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1659 GACGTC GACGT^C AccI 1679 GTAGAC GT^[AC][GT]AC AocI 96 CCTGAGG CC^T[ACGT]AGG ApaI 2621 GGGCCC GGGCC^C AseI 1810 ATTAAT AT^TAAT AsnI 1810 ATTAAT AT^TAAT BalI 1574 TGGCCA TGG^CCA BanI 689 GGCGCC G^G[CT][GA]CC BbeI 689 GGCGCC GGCGC^C BbsI 1717 GAAGAC GAAGAC(2/6) BcgI 1535 CGAATGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 541 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2121 AGATCT A^GATCT BpuAI 1717 GAAGAC GAAGAC(2/6) BsmI 2171 GAATGC GAATGC(1/-1) BspHI 2388 TCATGA T^CATGA BsrDI 1643 CATTGC GCAATG(2/0) Bsu36I 96 CCTGAGG CC^T[ACGT]AGG DraI 1853 TTTAAA TTT^AAA DraIII 2762 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 112 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 96 CCTGAGG CC^T[ACGT]AGG HgaI 200 GCGTC GACGC(5/10) KasI 689 GGCGCC G^GCGCC MfeI 2606 CAATTG C^AATTG MluNI 1574 TGGCCA TGG^CCA MscI 1574 TGGCCA TGG^CCA MunI 2606 CAATTG C^AATTG NarI 689 GGCGCC GG^CGCC NcoI 2180 CCATGG C^CATGG NdeI 2110 CATATG CA^TATG PflMI 2154 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1810 ATTAAT AT^TAAT RcaI 2388 TCATGA T^CATGA ScaI 2135 AGTACT AGT^ACT SfoI 689 GGCGCC GGC^GCC SmaI 2197 CCCGGG CCC^GGG StuI 77 AGGCCT AGG^CCT TaqI 1534 TCGA T^CGA TthHB8I 1534 TCGA T^CGA Van91I 2154 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2197 CCCGGG C^CCGGG XmaI 2197 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 689 GGCGCC G[GA]^CG[CT]C 1659 GACGTC AfaI 1566 GTAC GT^AC 2136 GTAC AflII 1171 CTTAAG C^TTAAG 1313 CTTAAG AlwNI 829 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 838 CAGGTTCTG AspHI 742 GTGCTC G[AT]GC[AT]^C 1332 GAGCAC BciVI 366 GGATAC GTATCC(6/5) 1344 GGATAC BfrI 1171 CTTAAG C^TTAAG 1313 CTTAAG BsaHI 689 GGCGCC G[GA]^CG[CT]C 1659 GACGTC BsiHKAI 742 GTGCTC G[AT]GC[AT]^C 1332 GAGCAC BssSI 1228 CACGAG CACGAG(-5/-1) 233 CTCGTG BstAPI 828 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2143 GCATAGCTTGC BstYI 2121 AGATCT [GA]^GATC[CT] 2295 AGATCC EaeI 1574 TGGCCA [CT]^GGCC[GA] 1592 CGGCCA FauI 432 CCCGC CCCGC(4/6) 2255 CCCGC Hin1I 689 GGCGCC G[GA]^CG[CT]C 1659 GACGTC MflI 2121 AGATCT [GA]^GATC[CT] 2295 AGATCC MslI 280 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2386 CATCATGATG PvuII 2245 CAGCTG CAG^CTG 2436 CAGCTG RsaI 1566 GTAC GT^AC 2136 GTAC SphI 41 GCATGC GCATG^C 279 GCATGC XbaI 1455 TCTAGA T^CTAGA 1872 TCTAGA XhoII 2121 AGATCT [GA]^GATC[CT] 2295 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AluI AlwI Aor51HI AvaI AvaII BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI