##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BciVI BfrI
BglII BlnI BpmI BsaAI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspEI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CfoI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MaeII MfeI MluI MroI
MunI NarI NdeI NheI NotI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI StuI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 1971 CGCG CG^CG
AflIII 2156 ACATGT A^C[GA][CT]GT
Alw44I 1583 GTGCAC G^TGCAC
AlwNI 1588 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1583 GTGCAC G^TGCAC
AseI 2493 ATTAAT AT^TAAT
AsnI 2493 ATTAAT AT^TAAT
Asp700I 1087 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 17 TGGCCA TGG^CCA
BamHI 209 GGATCC G^GATCC
BanII 1192 GAGCCC G[GA]GC[CT]^C
BcgI 257 GCAATAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 767 TGATCA T^GATCA
BcnI 706 CCCGG CC^[GC]GG
BglI 10 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1366 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC
BsaWI 1221 ACCGGA [AT]^CCGG[AT]
BsgI 529 CTGCAC GTGCAG(16/14)
BsmI 1062 GAATGC GAATGC(1/-1)
BspDI 559 ATCGAT AT^CGAT
BspLU11I 2156 ACATGT A^CATGT
BsrBI 2281 GAGCGG CCGCTC(-3/-3)
BstUI 1971 CGCG CG^CG
CelII 1366 GCTGAGC GC^T[ACGT]AGC
ClaI 559 ATCGAT AT^CGAT
DraII 1250 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 1827 GACTCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 17 TGGCCA [CT]^GGCC[GA]
EcoNI 1254 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1250 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 537 GAATTC G^AATTC
EcoRV 1290 GATATC GAT^ATC
FauI 1128 CCCGC CCCGC(4/6)
FbaI 767 TGATCA T^GATCA
HindIII 1035 AAGCTT A^AGCTT
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
MvnI 1971 CGCG CG^CG
NaeI 1023 GCCGGC GCC^GGC
NciI 706 CCCGG CC^[GC]GG
NgoMIV 1023 GCCGGC G^CCGGC
NruI 1970 TCGCGA TCG^CGA
NspI 2156 ACATGT [GA]CATG^[CT]
PflFI 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 999 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1250 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 2493 ATTAAT AT^TAAT
PvuII 2345 CAGCTG CAG^CTG
SapI 294 GCTCTTC GCTCTTC(1/4)
SpeI 100 ACTAGT A^CTAGT
Tth111I 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 999 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1087 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2630 GTCTAC GT^[AC][GT]AC
2880 GTAGAC
AvaI 736 CTCGAG C^[CT]CG[GA]G
1541 CTCGAG
BanI 1459 GGCACC G^G[CT][GA]CC
1734 GGTGCC
BbvCI 438 CCTCAGC CCTCAGC(-5/-2)
1131 GCTGAGG
BsaBI 624 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1110 GATCAGCATC
BsmAI 857 GAGAC GTCTC(1/5)
1799 GTCTC
BsmFI 110 GTCCC GGGAC(10/14)
775 GTCCC
BsoBI 736 CTCGAG C^[CT]CG[GA]G
1541 CTCGAG
BspMI 1507 ACCTGC ACCTGC(4/8)
1239 GCAGGT
BsrFI 1023 GCCGGC [GA]^CCGG[CT]
2568 ACCGGC
BstXI 173 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
269 CCAAGACCGTGG
BstYI 209 GGATCC [GA]^GATC[CT]
2610 AGATCC
Cfr10I 1023 GCCGGC [GA]^CCGG[CT]
2568 ACCGGC
DraI 862 TTTAAA TTT^AAA
2889 TTTAAA
EarI 295 CTCTTC CTCTTC(1/4)
1091 CTCTTC
HincII 2134 GTTGAC GT[CT]^[GA]AC
2687 GTTGAC
HindII 2134 GTTGAC GT[CT]^[GA]AC
2687 GTTGAC
Ksp632I 295 CTCTTC CTCTTC(1/4)
1091 CTCTTC
MamI 624 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1110 GATCAGCATC
MflI 209 GGATCC [GA]^GATC[CT]
2610 AGATCC
NcoI 14 CCATGG C^CATGG
1361 CCATGG
PaeR7I 736 CTCGAG C^TCGAG
1541 CTCGAG
PstI 286 CTGCAG CTGCA^G
1771 CTGCAG
XhoI 736 CTCGAG C^TCGAG
1541 CTCGAG
XhoII 209 GGATCC [GA]^GATC[CT]
2610 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI