##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AviII       
AvrII       BbeI        BbrPI       BciVI       BfrI        
BglII       BlnI        BpmI        BsaAI       BsaHI       
BsaI        BseAI       BseRI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspEI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CfoI        
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoT22I     
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
KspI        MaeII       MfeI        MluI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII        1971  CGCG                 CG^CG
AflIII       2156  ACATGT               A^C[GA][CT]GT
Alw44I       1583  GTGCAC               G^TGCAC
AlwNI        1588  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1583  GTGCAC               G^TGCAC
AseI         2493  ATTAAT               AT^TAAT
AsnI         2493  ATTAAT               AT^TAAT
Asp700I      1087  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1141  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI           17  TGGCCA               TGG^CCA
BamHI         209  GGATCC               G^GATCC
BanII        1192  GAGCCC               G[GA]GC[CT]^C
BcgI          257  GCAATAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          767  TGATCA               T^GATCA
BcnI          706  CCCGG                CC^[GC]GG
BglI           10  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         1366  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1366  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1221  ACCGGA               [AT]^CCGG[AT]
BsgI          529  CTGCAC               GTGCAG(16/14)
BsmI         1062  GAATGC               GAATGC(1/-1)
BspDI         559  ATCGAT               AT^CGAT
BspLU11I     2156  ACATGT               A^CATGT
BsrBI        2281  GAGCGG               CCGCTC(-3/-3)
BstUI        1971  CGCG                 CG^CG
CelII        1366  GCTGAGC              GC^T[ACGT]AGC
ClaI          559  ATCGAT               AT^CGAT
DraII        1250  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI         1827  GACTCAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           17  TGGCCA               [CT]^GGCC[GA]
EcoNI        1254  CCTAATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1250  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         537  GAATTC               G^AATTC
EcoRV        1290  GATATC               GAT^ATC
FauI         1128  CCCGC                CCCGC(4/6)
FbaI          767  TGATCA               T^GATCA
HindIII      1035  AAGCTT               A^AGCTT
MluNI          17  TGGCCA               TGG^CCA
MscI           17  TGGCCA               TGG^CCA
MvnI         1971  CGCG                 CG^CG
NaeI         1023  GCCGGC               GCC^GGC
NciI          706  CCCGG                CC^[GC]GG
NgoMIV       1023  GCCGGC               G^CCGGC
NruI         1970  TCGCGA               TCG^CGA
NspI         2156  ACATGT               [GA]CATG^[CT]
PflFI        1141  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         999  CCACCGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1250  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        2493  ATTAAT               AT^TAAT
PvuII        2345  CAGCTG               CAG^CTG
SapI          294  GCTCTTC              GCTCTTC(1/4)
SpeI          100  ACTAGT               A^CTAGT
Tth111I      1141  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        999  CCACCGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1087  GAATCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2630  GTCTAC               GT^[AC][GT]AC
             2880  GTAGAC              
AvaI          736  CTCGAG               C^[CT]CG[GA]G
             1541  CTCGAG              
BanI         1459  GGCACC               G^G[CT][GA]CC
             1734  GGTGCC              
BbvCI         438  CCTCAGC              CCTCAGC(-5/-2)
             1131  GCTGAGG             
BsaBI         624  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1110  GATCAGCATC          
BsmAI         857  GAGAC                GTCTC(1/5)
             1799  GTCTC               
BsmFI         110  GTCCC                GGGAC(10/14)
              775  GTCCC               
BsoBI         736  CTCGAG               C^[CT]CG[GA]G
             1541  CTCGAG              
BspMI        1507  ACCTGC               ACCTGC(4/8)
             1239  GCAGGT              
BsrFI        1023  GCCGGC               [GA]^CCGG[CT]
             2568  ACCGGC              
BstXI         173  CCACCACCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              269  CCAAGACCGTGG        
BstYI         209  GGATCC               [GA]^GATC[CT]
             2610  AGATCC              
Cfr10I       1023  GCCGGC               [GA]^CCGG[CT]
             2568  ACCGGC              
DraI          862  TTTAAA               TTT^AAA
             2889  TTTAAA              
EarI          295  CTCTTC               CTCTTC(1/4)
             1091  CTCTTC              
HincII       2134  GTTGAC               GT[CT]^[GA]AC
             2687  GTTGAC              
HindII       2134  GTTGAC               GT[CT]^[GA]AC
             2687  GTTGAC              
Ksp632I       295  CTCTTC               CTCTTC(1/4)
             1091  CTCTTC              
MamI          624  GATTGGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1110  GATCAGCATC          
MflI          209  GGATCC               [GA]^GATC[CT]
             2610  AGATCC              
NcoI           14  CCATGG               C^CATGG
             1361  CCATGG              
PaeR7I        736  CTCGAG               C^TCGAG
             1541  CTCGAG              
PstI          286  CTGCAG               CTGCA^G
             1771  CTGCAG              
XhoI          736  CTCGAG               C^TCGAG
             1541  CTCGAG              
XhoII         209  GGATCC               [GA]^GATC[CT]
             2610  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbsI        BbvI        
BfaI        BmyI        BpuAI       BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BspHI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XbaI        XcmI