################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BciVI BfrI BglII BlnI BpmI BsaAI BsaHI BsaI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspEI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CfoI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MfeI MluI MroI MunI NarI NdeI NheI NotI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 1971 CGCG CG^CG AflIII 2156 ACATGT A^C[GA][CT]GT Alw44I 1583 GTGCAC G^TGCAC AlwNI 1588 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1583 GTGCAC G^TGCAC AseI 2493 ATTAAT AT^TAAT AsnI 2493 ATTAAT AT^TAAT Asp700I 1087 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 17 TGGCCA TGG^CCA BamHI 209 GGATCC G^GATCC BanII 1192 GAGCCC G[GA]GC[CT]^C BcgI 257 GCAATAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 767 TGATCA T^GATCA BcnI 706 CCCGG CC^[GC]GG BglI 10 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1366 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1366 GCTGAGC GC^T[ACGT]AGC BsaWI 1221 ACCGGA [AT]^CCGG[AT] BsgI 529 CTGCAC GTGCAG(16/14) BsmI 1062 GAATGC GAATGC(1/-1) BspDI 559 ATCGAT AT^CGAT BspLU11I 2156 ACATGT A^CATGT BsrBI 2281 GAGCGG CCGCTC(-3/-3) BstUI 1971 CGCG CG^CG CelII 1366 GCTGAGC GC^T[ACGT]AGC ClaI 559 ATCGAT AT^CGAT DraII 1250 AGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 1827 GACTCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 17 TGGCCA [CT]^GGCC[GA] EcoNI 1254 CCTAATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1250 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 537 GAATTC G^AATTC EcoRV 1290 GATATC GAT^ATC FauI 1128 CCCGC CCCGC(4/6) FbaI 767 TGATCA T^GATCA HindIII 1035 AAGCTT A^AGCTT MluNI 17 TGGCCA TGG^CCA MscI 17 TGGCCA TGG^CCA MvnI 1971 CGCG CG^CG NaeI 1023 GCCGGC GCC^GGC NciI 706 CCCGG CC^[GC]GG NgoMIV 1023 GCCGGC G^CCGGC NruI 1970 TCGCGA TCG^CGA NspI 2156 ACATGT [GA]CATG^[CT] PflFI 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 999 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1250 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 2493 ATTAAT AT^TAAT PvuII 2345 CAGCTG CAG^CTG SapI 294 GCTCTTC GCTCTTC(1/4) SpeI 100 ACTAGT A^CTAGT Tth111I 1141 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 999 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1087 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 2630 GTCTAC GT^[AC][GT]AC 2880 GTAGAC AvaI 736 CTCGAG C^[CT]CG[GA]G 1541 CTCGAG BanI 1459 GGCACC G^G[CT][GA]CC 1734 GGTGCC BbvCI 438 CCTCAGC CCTCAGC(-5/-2) 1131 GCTGAGG BsaBI 624 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1110 GATCAGCATC BsmAI 857 GAGAC GTCTC(1/5) 1799 GTCTC BsmFI 110 GTCCC GGGAC(10/14) 775 GTCCC BsoBI 736 CTCGAG C^[CT]CG[GA]G 1541 CTCGAG BspMI 1507 ACCTGC ACCTGC(4/8) 1239 GCAGGT BsrFI 1023 GCCGGC [GA]^CCGG[CT] 2568 ACCGGC BstXI 173 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 269 CCAAGACCGTGG BstYI 209 GGATCC [GA]^GATC[CT] 2610 AGATCC Cfr10I 1023 GCCGGC [GA]^CCGG[CT] 2568 ACCGGC DraI 862 TTTAAA TTT^AAA 2889 TTTAAA EarI 295 CTCTTC CTCTTC(1/4) 1091 CTCTTC HincII 2134 GTTGAC GT[CT]^[GA]AC 2687 GTTGAC HindII 2134 GTTGAC GT[CT]^[GA]AC 2687 GTTGAC Ksp632I 295 CTCTTC CTCTTC(1/4) 1091 CTCTTC MamI 624 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1110 GATCAGCATC MflI 209 GGATCC [GA]^GATC[CT] 2610 AGATCC NcoI 14 CCATGG C^CATGG 1361 CCATGG PaeR7I 736 CTCGAG C^TCGAG 1541 CTCGAG PstI 286 CTGCAG CTGCA^G 1771 CTGCAG XhoI 736 CTCGAG C^TCGAG 1541 CTCGAG XhoII 209 GGATCC [GA]^GATC[CT] 2610 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspHI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI