##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AocI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BglI BglII
BlnI BsaAI BsaHI BseAI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
ClaI CpoI DrdI Eco47III Eco81I
EcoRI EcoRV FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflIII 467 ACATGT A^C[GA][CT]GT
AhdI 2340 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 642 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2340 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 178 GGCACC G^G[CT][GA]CC
BbvCI 182 CCTCAGC CCTCAGC(-5/-2)
BclI 122 TGATCA T^GATCA
BlpI 1751 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1751 GCTCAGC GC^T[ACGT]AGC
BsaBI 475 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1505 ACCGGA [AT]^CCGG[AT]
BsmBI 1128 GAGACG CGTCTC(1/5)
Bsp1407I 1173 TGTACA T^GTACA
BspLU11I 467 ACATGT A^CATGT
BsrBI 1784 CCGCTC CCGCTC(-3/-3)
BsrDI 2045 GCAATG GCAATG(2/0)
BsrFI 803 GCCGGC [GA]^CCGG[CT]
BsrGI 1173 TGTACA T^GTACA
BstEII 1038 GGTGACC G^GT[ACGT]ACC
BstPI 1038 GGTGACC G^GT[ACGT]ACC
BstXI 2396 CCACCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1751 GCTCAGC GC^T[ACGT]AGC
Cfr10I 803 GCCGGC [GA]^CCGG[CT]
DraI 2651 TTTAAA TTT^AAA
DraIII 347 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2571 CGGCCG C^GGCCG
Eam1105I 2340 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2571 CGGCCG C^GGCCG
Eco52I 2571 CGGCCG C^GGCCG
EcoO65I 1038 GGTGACC G^GT[ACGT]ACC
EcoT22I 885 ATGCAT ATGCA^T
FbaI 122 TGATCA T^GATCA
HaeII 2533 GGCGCT [GA]GCGC^[CT]
HgaI 1250 GACGC GACGC(5/10)
MaeII 1131 ACGT A^CGT
MamI 475 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 803 GCCGGC GCC^GGC
NgoMIV 803 GCCGGC G^CCGGC
NsiI 885 ATGCAT ATGCA^T
NspI 467 ACATGT [GA]CATG^[CT]
PvuI 2444 CGATCG CGAT^CG
SacI 796 GAGCTC GAGCT^C
SmaI 1655 CCCGGG CCC^GGG
SmlI 1338 CTCAAG C^T[CT][GA]AG
SspBI 1173 TGTACA T^GTACA
StuI 1394 AGGCCT AGG^CCT
TfiI 1831 GATTC G^A[AT]TC
XmaCI 1655 CCCGGG C^CCGGG
XmaI 1655 CCCGGG C^CCGGG
XmnI 642 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 1191 GGGCCC GGGCC^C
2008 GGGCCC
AvaI 914 CTCGGG C^[CT]CG[GA]G
1655 CCCGGG
BalI 1664 TGGCCA TGG^CCA
2058 TGGCCA
BbsI 1743 GAAGAC GAAGAC(2/6)
734 GTCTTC
BpuAI 1743 GAAGAC GAAGAC(2/6)
734 GTCTTC
BsiEI 2444 CGATCG CG[GA][CT]^CG
2571 CGGCCG
BsmI 726 GAATGC GAATGC(1/-1)
1713 GAATGC
BsoBI 914 CTCGGG C^[CT]CG[GA]G
1655 CCCGGG
BstAPI 513 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2197 GCAGCGGGTGC
EcoNI 1182 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1386 CCTTCAGCAGG
MluNI 1664 TGGCCA TGG^CCA
2058 TGGCCA
MscI 1664 TGGCCA TGG^CCA
2058 TGGCCA
NcoI 634 CCATGG C^CATGG
815 CCATGG
PpuMI 248 AGGTCCT [GA]G^G[AT]CC[CT]
1812 AGGTCCT
PvuII 216 CAGCTG CAG^CTG
388 CAGCTG
SexAI 1310 ACCTGGT A^CC[AT]GGT
2618 ACCTGGT
TaqI 629 TCGA T^CGA
1644 TCGA
TthHB8I 629 TCGA T^CGA
1644 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaII BamHI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII