##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI AlwNI
ApaI AscI Asp718I AspEI AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BlnI BpuAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI Hin1I KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 168 GTCTAC GT^[AC][GT]AC
AflII 1100 CTTAAG C^TTAAG
AocI 2706 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1939 AGCGCT AGC^GCT
AseI 1080 ATTAAT AT^TAAT
AsnI 1080 ATTAAT AT^TAAT
Asp700I 2542 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 265 CCCGAG C^[CT]CG[GA]G
AviII 235 TGCGCA TGC^GCA
BfrI 1100 CTTAAG C^TTAAG
BglI 1169 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2523 TACGTG [CT]AC^GT[GA]
BsaBI 3038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 675 ACCGGA [AT]^CCGG[AT]
BsoBI 265 CCCGAG C^[CT]CG[GA]G
BsrBI 347 GAGCGG CCGCTC(-3/-3)
BstAPI 302 GCAAGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2800 CCATGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2706 CCTCAGG CC^T[ACGT]AGG
Eco47III 1939 AGCGCT AGC^GCT
Eco81I 2706 CCTCAGG CC^T[ACGT]AGG
EcoRV 1914 GATATC GAT^ATC
FspI 235 TGCGCA TGC^GCA
HgaI 59 GACGC GACGC(5/10)
HpaI 2789 GTTAAC GTT^AAC
MamI 3038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2816 CAATTG C^AATTG
MunI 2816 CAATTG C^AATTG
NcoI 1634 CCATGG C^CATGG
PflFI 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1650 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1080 ATTAAT AT^TAAT
SmlI 1100 CTTAAG C^T[CT][GA]AG
SspI 1083 AATATT AAT^ATT
Tth111I 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1650 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2542 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 2511 GTGCAC G^TGCAC
2526 GTGCAC
ApaLI 2511 GTGCAC G^TGCAC
2526 GTGCAC
BanI 308 GGTGCC G^G[CT][GA]CC
484 GGTGCC
BanII 1872 GAGCCC G[GA]GC[CT]^C
2810 GGGCTC
BciVI 3037 GGATAC GTATCC(6/5)
2928 GTATCC
BlpI 555 GCTAAGC GC^T[ACGT]AGC
560 GCTTAGC
Bpu1102I 555 GCTAAGC GC^T[ACGT]AGC
560 GCTTAGC
BsgI 51 GTGCAG GTGCAG(16/14)
737 GTGCAG
CelII 555 GCTAAGC GC^T[ACGT]AGC
560 GCTTAGC
DsaI 140 CCACGG C^C[GA][CT]GG
1634 CCATGG
EcoNI 107 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
794 CCTTGAATAGG
HaeII 966 AGCGCC [GA]GCGC^[CT]
1939 AGCGCT
HapII 131 CCGG C^CGG
676 CCGG
HindIII 558 AAGCTT A^AGCTT
2901 AAGCTT
HpaII 131 CCGG C^CGG
676 CCGG
MaeII 2156 ACGT A^CGT
2524 ACGT
MspA1I 30 CAGCTG C[AC]G^C[GT]G
146 CAGCTG
MspI 131 CCGG C^CGG
676 CCGG
NspI 576 GCATGC [GA]CATG^[CT]
1527 GCATGC
PleI 127 GAGTC GAGTC(4/5)
2039 GAGTC
PstI 18 CTGCAG CTGCA^G
1749 CTGCAG
PvuII 30 CAGCTG CAG^CTG
146 CAGCTG
SphI 576 GCATGC GCATG^C
1527 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BbvI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII