################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI AlwNI ApaI AscI Asp718I AspEI AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BlnI BpuAI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoT22I FbaI FseI Hin1I KasI KpnI KspI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 168 GTCTAC GT^[AC][GT]AC AflII 1100 CTTAAG C^TTAAG AocI 2706 CCTCAGG CC^T[ACGT]AGG Aor51HI 1939 AGCGCT AGC^GCT AseI 1080 ATTAAT AT^TAAT AsnI 1080 ATTAAT AT^TAAT Asp700I 2542 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 265 CCCGAG C^[CT]CG[GA]G AviII 235 TGCGCA TGC^GCA BfrI 1100 CTTAAG C^TTAAG BglI 1169 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2523 TACGTG [CT]AC^GT[GA] BsaBI 3038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 675 ACCGGA [AT]^CCGG[AT] BsoBI 265 CCCGAG C^[CT]CG[GA]G BsrBI 347 GAGCGG CCGCTC(-3/-3) BstAPI 302 GCAAGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2800 CCATGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2706 CCTCAGG CC^T[ACGT]AGG Eco47III 1939 AGCGCT AGC^GCT Eco81I 2706 CCTCAGG CC^T[ACGT]AGG EcoRV 1914 GATATC GAT^ATC FspI 235 TGCGCA TGC^GCA HgaI 59 GACGC GACGC(5/10) HpaI 2789 GTTAAC GTT^AAC MamI 3038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2816 CAATTG C^AATTG MunI 2816 CAATTG C^AATTG NcoI 1634 CCATGG C^CATGG PflFI 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1650 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1080 ATTAAT AT^TAAT SmlI 1100 CTTAAG C^T[CT][GA]AG SspI 1083 AATATT AAT^ATT Tth111I 1403 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1650 CCACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2542 GAATTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Alw44I 2511 GTGCAC G^TGCAC 2526 GTGCAC ApaLI 2511 GTGCAC G^TGCAC 2526 GTGCAC BanI 308 GGTGCC G^G[CT][GA]CC 484 GGTGCC BanII 1872 GAGCCC G[GA]GC[CT]^C 2810 GGGCTC BciVI 3037 GGATAC GTATCC(6/5) 2928 GTATCC BlpI 555 GCTAAGC GC^T[ACGT]AGC 560 GCTTAGC Bpu1102I 555 GCTAAGC GC^T[ACGT]AGC 560 GCTTAGC BsgI 51 GTGCAG GTGCAG(16/14) 737 GTGCAG CelII 555 GCTAAGC GC^T[ACGT]AGC 560 GCTTAGC DsaI 140 CCACGG C^C[GA][CT]GG 1634 CCATGG EcoNI 107 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 794 CCTTGAATAGG HaeII 966 AGCGCC [GA]GCGC^[CT] 1939 AGCGCT HapII 131 CCGG C^CGG 676 CCGG HindIII 558 AAGCTT A^AGCTT 2901 AAGCTT HpaII 131 CCGG C^CGG 676 CCGG MaeII 2156 ACGT A^CGT 2524 ACGT MspA1I 30 CAGCTG C[AC]G^C[GT]G 146 CAGCTG MspI 131 CCGG C^CGG 676 CCGG NspI 576 GCATGC [GA]CATG^[CT] 1527 GCATGC PleI 127 GAGTC GAGTC(4/5) 2039 GAGTC PstI 18 CTGCAG CTGCA^G 1749 CTGCAG PvuII 30 CAGCTG CAG^CTG 146 CAGCTG SphI 576 GCATGC GCATG^C 1527 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BbvI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII