##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI AscI AspEI AvaI
AviII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FbaI FseI FspI HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1798 GGTACC G^GTACC
AccI 1603 GTCTAC GT^[AC][GT]AC
AccII 705 CGCG CG^CG
AlwNI 2019 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 158 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1131 AGCGCT AGC^GCT
ApaI 111 GGGCCC GGGCC^C
AseI 1745 ATTAAT AT^TAAT
AsnI 1745 ATTAAT AT^TAAT
Asp700I 248 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1798 GGTACC G^GTACC
AvrII 3074 CCTAGG C^CTAGG
BalI 351 TGGCCA TGG^CCA
BamHI 2976 GGATCC G^GATCC
BanI 1798 GGTACC G^G[CT][GA]CC
BglI 285 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1981 AGATCT A^GATCT
BlnI 3074 CCTAGG C^CTAGG
BspDI 861 ATCGAT AT^CGAT
BspHI 556 TCATGA T^CATGA
BssSI 2436 CACGAG CACGAG(-5/-1)
BstEII 362 GGTGACC G^GT[ACGT]ACC
BstPI 362 GGTGACC G^GT[ACGT]ACC
BstUI 705 CGCG CG^CG
Bsu36I 158 CCTCAGG CC^T[ACGT]AGG
ClaI 861 ATCGAT AT^CGAT
DrdI 2868 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 351 TGGCCA [CT]^GGCC[GA]
Eco47III 1131 AGCGCT AGC^GCT
Eco81I 158 CCTCAGG CC^T[ACGT]AGG
EcoO65I 362 GGTGACC G^GT[ACGT]ACC
EcoRI 2957 GAATTC G^AATTC
FauI 808 CCCGC CCCGC(4/6)
HaeII 1131 AGCGCT [GA]GCGC^[CT]
HincII 1558 GTTGAC GT[CT]^[GA]AC
HindII 1558 GTTGAC GT[CT]^[GA]AC
HindIII 1910 AAGCTT A^AGCTT
KpnI 1798 GGTACC GGTAC^C
MluNI 351 TGGCCA TGG^CCA
MscI 351 TGGCCA TGG^CCA
MvnI 705 CGCG CG^CG
NdeI 2253 CATATG CA^TATG
NheI 1234 GCTAGC G^CTAGC
PshBI 1745 ATTAAT AT^TAAT
PstI 843 CTGCAG CTGCA^G
RcaI 556 TCATGA T^CATGA
SexAI 145 ACCTGGT A^CC[AT]GGT
SfcI 843 CTGCAG C^T[GA][CT]AG
SmlI 1411 CTTGAG C^T[CT][GA]AG
StuI 1179 AGGCCT AGG^CCT
XmnI 248 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 885 GAATTT [GA]^AATT[CT]
2957 GAATTC
AflIII 3447 ACATGT A^C[GA][CT]GT
3561 ACATGT
Alw44I 1143 GTGCAC G^TGCAC
3557 GTGCAC
ApaLI 1143 GTGCAC G^TGCAC
3557 GTGCAC
ApoI 885 GAATTT [GA]^AATT[CT]
2957 GAATTC
AspI 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2017 GACAGTGTC
BspLU11I 3447 ACATGT A^CATGT
3561 ACATGT
BspMI 1104 GCAGGT ACCTGC(4/8)
3262 GCAGGT
BsrDI 791 CATTGC GCAATG(2/0)
1870 CATTGC
BstYI 1981 AGATCT [GA]^GATC[CT]
2976 GGATCC
CfoI 704 GCGC GCG^C
1132 GCGC
DraIII 2436 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3551 CACAATGTG
EcoNI 445 CCTTAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3501 CCTCCTGGAGG
EcoT22I 2176 ATGCAT ATGCA^T
3041 ATGCAT
HhaI 704 GCGC GCG^C
1132 GCGC
HinP1I 704 GCGC G^CGC
1132 GCGC
MflI 1981 AGATCT [GA]^GATC[CT]
2976 GGATCC
MslI 1576 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3089 CATGTAAATG
NcoI 418 CCATGG C^CATGG
2012 CCATGG
NsiI 2176 ATGCAT ATGCA^T
3041 ATGCAT
PflFI 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2017 GACAGTGTC
PpuMI 690 AGGTCCT [GA]G^G[AT]CC[CT]
3034 GGGACCC
SacI 941 GAGCTC GAGCT^C
992 GAGCTC
SapI 2291 GAAGAGC GCTCTTC(1/4)
38 GCTCTTC
Tth111I 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2017 GACAGTGTC
XbaI 1674 TCTAGA T^CTAGA
3251 TCTAGA
XhoII 1981 AGATCT [GA]^GATC[CT]
2976 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI