################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI AscI AspEI AvaI AviII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I CpoI EagI Eam1105I EclXI Eco52I EcoRV FbaI FseI FspI HgaI Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1798 GGTACC G^GTACC AccI 1603 GTCTAC GT^[AC][GT]AC AccII 705 CGCG CG^CG AlwNI 2019 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 158 CCTCAGG CC^T[ACGT]AGG Aor51HI 1131 AGCGCT AGC^GCT ApaI 111 GGGCCC GGGCC^C AseI 1745 ATTAAT AT^TAAT AsnI 1745 ATTAAT AT^TAAT Asp700I 248 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1798 GGTACC G^GTACC AvrII 3074 CCTAGG C^CTAGG BalI 351 TGGCCA TGG^CCA BamHI 2976 GGATCC G^GATCC BanI 1798 GGTACC G^G[CT][GA]CC BglI 285 GCCCACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1981 AGATCT A^GATCT BlnI 3074 CCTAGG C^CTAGG BspDI 861 ATCGAT AT^CGAT BspHI 556 TCATGA T^CATGA BssSI 2436 CACGAG CACGAG(-5/-1) BstEII 362 GGTGACC G^GT[ACGT]ACC BstPI 362 GGTGACC G^GT[ACGT]ACC BstUI 705 CGCG CG^CG Bsu36I 158 CCTCAGG CC^T[ACGT]AGG ClaI 861 ATCGAT AT^CGAT DrdI 2868 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 351 TGGCCA [CT]^GGCC[GA] Eco47III 1131 AGCGCT AGC^GCT Eco81I 158 CCTCAGG CC^T[ACGT]AGG EcoO65I 362 GGTGACC G^GT[ACGT]ACC EcoRI 2957 GAATTC G^AATTC FauI 808 CCCGC CCCGC(4/6) HaeII 1131 AGCGCT [GA]GCGC^[CT] HincII 1558 GTTGAC GT[CT]^[GA]AC HindII 1558 GTTGAC GT[CT]^[GA]AC HindIII 1910 AAGCTT A^AGCTT KpnI 1798 GGTACC GGTAC^C MluNI 351 TGGCCA TGG^CCA MscI 351 TGGCCA TGG^CCA MvnI 705 CGCG CG^CG NdeI 2253 CATATG CA^TATG NheI 1234 GCTAGC G^CTAGC PshBI 1745 ATTAAT AT^TAAT PstI 843 CTGCAG CTGCA^G RcaI 556 TCATGA T^CATGA SexAI 145 ACCTGGT A^CC[AT]GGT SfcI 843 CTGCAG C^T[GA][CT]AG SmlI 1411 CTTGAG C^T[CT][GA]AG StuI 1179 AGGCCT AGG^CCT XmnI 248 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 885 GAATTT [GA]^AATT[CT] 2957 GAATTC AflIII 3447 ACATGT A^C[GA][CT]GT 3561 ACATGT Alw44I 1143 GTGCAC G^TGCAC 3557 GTGCAC ApaLI 1143 GTGCAC G^TGCAC 3557 GTGCAC ApoI 885 GAATTT [GA]^AATT[CT] 2957 GAATTC AspI 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2017 GACAGTGTC BspLU11I 3447 ACATGT A^CATGT 3561 ACATGT BspMI 1104 GCAGGT ACCTGC(4/8) 3262 GCAGGT BsrDI 791 CATTGC GCAATG(2/0) 1870 CATTGC BstYI 1981 AGATCT [GA]^GATC[CT] 2976 GGATCC CfoI 704 GCGC GCG^C 1132 GCGC DraIII 2436 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG 3551 CACAATGTG EcoNI 445 CCTTAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3501 CCTCCTGGAGG EcoT22I 2176 ATGCAT ATGCA^T 3041 ATGCAT HhaI 704 GCGC GCG^C 1132 GCGC HinP1I 704 GCGC G^CGC 1132 GCGC MflI 1981 AGATCT [GA]^GATC[CT] 2976 GGATCC MslI 1576 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3089 CATGTAAATG NcoI 418 CCATGG C^CATGG 2012 CCATGG NsiI 2176 ATGCAT ATGCA^T 3041 ATGCAT PflFI 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2017 GACAGTGTC PpuMI 690 AGGTCCT [GA]G^G[AT]CC[CT] 3034 GGGACCC SacI 941 GAGCTC GAGCT^C 992 GAGCTC SapI 2291 GAAGAGC GCTCTTC(1/4) 38 GCTCTTC Tth111I 144 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 2017 GACAGTGTC XbaI 1674 TCTAGA T^CTAGA 3251 TCTAGA XhoII 1981 AGATCT [GA]^GATC[CT] 2976 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI