##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
BamHI BanI BbeI BcgI BciVI
BclI BcnI BglI BglII BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HaeII
Hin1I KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MunI NarI NciI NdeI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 931 GTAGAC GT^[AC][GT]AC
AhdI 2048 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1335 GGGCCC GGGCC^C
AspEI 2048 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1709 TGCGCA TGC^GCA
AvrII 1663 CCTAGG C^CTAGG
BbrPI 1541 CACGTG CAC^GTG
BlnI 1663 CCTAGG C^CTAGG
BsaAI 1541 CACGTG [CT]AC^GT[GA]
BsrFI 1272 GCCGGC [GA]^CCGG[CT]
BstAPI 682 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1030 TTCGAA TT^CGAA
BstXI 2656 CCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1272 GCCGGC [GA]^CCGG[CT]
DraIII 2058 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 58 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2048 GACTTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1420 CCTCTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2103 ATGCAT ATGCA^T
FspI 1709 TGCGCA TGC^GCA
HapII 1273 CCGG C^CGG
HgaI 1793 GCGTC GACGC(5/10)
HincII 127 GTTAAC GT[CT]^[GA]AC
HindII 127 GTTAAC GT[CT]^[GA]AC
HpaI 127 GTTAAC GTT^AAC
HpaII 1273 CCGG C^CGG
MspI 1273 CCGG C^CGG
NaeI 1272 GCCGGC GCC^GGC
NgoMIV 1272 GCCGGC G^CCGGC
NheI 2090 GCTAGC G^CTAGC
NsiI 2103 ATGCAT ATGCA^T
NspV 1030 TTCGAA TT^CGAA
PflMI 2155 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1541 CACGTG CAC^GTG
PmlI 1541 CACGTG CAC^GTG
PshAI 1376 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1030 TTCGAA TT^CGAA
Van91I 2155 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 45 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AccII 4 CGCG CG^CG
1792 CGCG
AflII 1261 CTTAAG C^TTAAG
2788 CTTAAG
AflIII 1540 ACACGT A^C[GA][CT]GT
1542 ACGTGT
AlwI 1096 GATCC GGATC(4/5)
1512 GGATC
AlwNI 821 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1516 CAGATGCTG
Asp700I 1670 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2198 GAATGCTTTC
AspHI 188 GAGCTC G[AT]GC[AT]^C
455 GAGCTC
AvaI 665 CCCGAG C^[CT]CG[GA]G
1432 CTCGGG
AvaII 72 GGACC G^G[AT]CC
553 GGTCC
BalI 546 TGGCCA TGG^CCA
2065 TGGCCA
BbvCI 1277 CCTCAGC CCTCAGC(-5/-2)
2543 GCTGAGG
BfrI 1261 CTTAAG C^TTAAG
2788 CTTAAG
BlpI 102 GCTAAGC GC^T[ACGT]AGC
190 GCTCAGC
Bpu1102I 102 GCTAAGC GC^T[ACGT]AGC
190 GCTCAGC
BsiHKAI 188 GAGCTC G[AT]GC[AT]^C
455 GAGCTC
BsmBI 1794 CGTCTC CGTCTC(1/5)
1155 GAGACG
BsmI 2198 GAATGC GAATGC(1/-1)
2777 GAATGC
BsoBI 665 CCCGAG C^[CT]CG[GA]G
1432 CTCGGG
Bsp1407I 326 TGTACA T^GTACA
2170 TGTACA
BspMI 558 ACCTGC ACCTGC(4/8)
1687 ACCTGC
BsrBI 1988 CCGCTC CCGCTC(-3/-3)
1743 GAGCGG
BsrDI 2843 CATTGC GCAATG(2/0)
748 GCAATG
BsrGI 326 TGTACA T^GTACA
2170 TGTACA
BstUI 4 CGCG CG^CG
1792 CGCG
CelII 102 GCTAAGC GC^T[ACGT]AGC
190 GCTCAGC
CfoI 3 GCGC GCG^C
1710 GCGC
DraI 1606 TTTAAA TTT^AAA
2902 TTTAAA
DraII 1334 AGGGCCC [GA]G^G[ACGT]CC[CT]
1335 GGGCCCC
DsaI 26 CCATGG C^C[GA][CT]GG
549 CCATGG
EaeI 546 TGGCCA [CT]^GGCC[GA]
2065 TGGCCA
EcoO109I 1334 AGGGCCC [GA]G^G[ACGT]CC[CT]
1335 GGGCCCC
EcoRI 1670 GAATTC G^AATTC
2813 GAATTC
FauI 5 GCGGG CCCGC(4/6)
1745 GCGGG
HhaI 3 GCGC GCG^C
1710 GCGC
HinP1I 3 GCGC G^CGC
1710 GCGC
HindIII 994 AAGCTT A^AGCTT
2791 AAGCTT
MaeII 1542 ACGT A^CGT
2762 ACGT
MluNI 546 TGGCCA TGG^CCA
2065 TGGCCA
MscI 546 TGGCCA TGG^CCA
2065 TGGCCA
MslI 21 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
318 CATCCATGTG
MvnI 4 CGCG CG^CG
1792 CGCG
NcoI 26 CCATGG C^CATGG
549 CCATGG
SacI 188 GAGCTC GAGCT^C
455 GAGCTC
SspBI 326 TGTACA T^GTACA
2170 TGTACA
XcmI 587 CCAAAGGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2940 CCAAATTTGAATTGG
XmnI 1670 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2198 GAATGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I