##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AflII Alw44I ApaLI
AscI AspI AviII BalI BbrPI
BcgI BclI BfrI BglII BsaAI
BsaBI BsiEI BsiWI BspDI BspHI
BsrDI BssSI BstAPI BstBI BstEII
BstPI ClaI CpoI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MaeII MamI
MluI MluNI MscI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1253 GGTACC G^GTACC
AccIII 726 TCCGGA T^CCGGA
AcsI 1387 AAATTC [GA]^AATT[CT]
AgeI 534 ACCGGT A^CCGGT
ApaI 2319 GGGCCC GGGCC^C
ApoI 1387 AAATTC [GA]^AATT[CT]
Asp700I 593 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1253 GGTACC G^GTACC
AspHI 1916 GAGCAC G[AT]GC[AT]^C
AvrII 1671 CCTAGG C^CTAGG
BanII 2319 GGGCCC G[GA]GC[CT]^C
BbeI 4 GGCGCC GGCGC^C
BciVI 409 GGATAC GTATCC(6/5)
BglI 37 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1671 CCTAGG C^CTAGG
BlpI 1864 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1864 GCTCAGC GC^T[ACGT]AGC
BsaI 1063 GAGACC GGTCTC(1/5)
BseAI 726 TCCGGA T^CCGGA
BsiHKAI 1916 GAGCAC G[AT]GC[AT]^C
BsmBI 890 GAGACG CGTCTC(1/5)
BspEI 726 TCCGGA T^CCGGA
BspMI 1624 ACCTGC ACCTGC(4/8)
BssHII 11 GCGCGC G^CGCGC
Bst1107I 579 GTATAC GTA^TAC
BstZ17I 579 GTATAC GTA^TAC
CelII 1864 GCTCAGC GC^T[ACGT]AGC
EcoRV 2171 GATATC GAT^ATC
HindIII 2587 AAGCTT A^AGCTT
KasI 4 GGCGCC G^GCGCC
KpnI 1253 GGTACC GGTAC^C
MfeI 1043 CAATTG C^AATTG
MroI 726 TCCGGA T^CCGGA
MunI 1043 CAATTG C^AATTG
NaeI 109 GCCGGC GCC^GGC
NarI 4 GGCGCC GG^CGCC
NgoMIV 109 GCCGGC G^CCGGC
NheI 1860 GCTAGC G^CTAGC
PinAI 534 ACCGGT A^CCGGT
PstI 307 CTGCAG CTGCA^G
SapI 635 GAAGAGC GCTCTTC(1/4)
SexAI 266 ACCTGGT A^CC[AT]GGT
SfiI 36 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4 GGCGCC GGC^GCC
SmaI 76 CCCGGG CCC^GGG
SmlI 667 CTCAAG C^T[CT][GA]AG
SspI 2298 AATATT AAT^ATT
StuI 921 AGGCCT AGG^CCT
SwaI 2225 ATTTAAAT ATTT^AAAT
TfiI 2641 GATTC G^A[AT]TC
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
XmnI 593 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 579 GTATAC GT^[AC][GT]AC
1700 GTCTAC
AcyI 4 GGCGCC G[GA]^CG[CT]C
166 GACGCC
AhdI 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACCTCCTGTC
AocI 741 CCTCAGG CC^T[ACGT]AGG
1751 CCTGAGG
Aor51HI 335 AGCGCT AGC^GCT
1094 AGCGCT
AseI 1402 ATTAAT AT^TAAT
1692 ATTAAT
AsnI 1402 ATTAAT AT^TAAT
1692 ATTAAT
AspEI 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACCTCCTGTC
AvaI 32 CTCGGG C^[CT]CG[GA]G
76 CCCGGG
BamHI 989 GGATCC G^GATCC
2150 GGATCC
BanI 4 GGCGCC G^G[CT][GA]CC
1253 GGTACC
BbsI 1572 GAAGAC GAAGAC(2/6)
2426 GTCTTC
BcnI 76 CCCGG CC^[GC]GG
77 CCGGG
BpuAI 1572 GAAGAC GAAGAC(2/6)
2426 GTCTTC
BsaHI 4 GGCGCC G[GA]^CG[CT]C
166 GACGCC
BsaWI 534 ACCGGT [AT]^CCGG[AT]
726 TCCGGA
BsgI 1179 GTGCAG GTGCAG(16/14)
1790 GTGCAG
BsmI 907 GAATGC GAATGC(1/-1)
1801 GCATTC
BsoBI 32 CTCGGG C^[CT]CG[GA]G
76 CCCGGG
Bsp1407I 362 TGTACA T^GTACA
502 TGTACA
BsrFI 109 GCCGGC [GA]^CCGG[CT]
534 ACCGGT
BsrGI 362 TGTACA T^GTACA
502 TGTACA
BstXI 260 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
483 CCAGGCAACTGG
BstYI 989 GGATCC [GA]^GATC[CT]
2150 GGATCC
Bsu36I 741 CCTCAGG CC^T[ACGT]AGG
1751 CCTGAGG
Cfr10I 109 GCCGGC [GA]^CCGG[CT]
534 ACCGGT
DsaI 191 CCGTGG C^C[GA][CT]GG
205 CCGTGG
EaeI 182 TGGCCG [CT]^GGCC[GA]
2113 CGGCCA
Eam1105I 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1114 GACCTCCTGTC
Eco47III 335 AGCGCT AGC^GCT
1094 AGCGCT
Eco81I 741 CCTCAGG CC^T[ACGT]AGG
1751 CCTGAGG
EcoNI 1780 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2362 CCTCTCTCAGG
Hin1I 4 GGCGCC G[GA]^CG[CT]C
166 GACGCC
MflI 989 GGATCC [GA]^GATC[CT]
2150 GGATCC
NciI 76 CCCGG CC^[GC]GG
77 CCGGG
PflMI 817 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2282 CCAGATGTTGG
PshBI 1402 ATTAAT AT^TAAT
1692 ATTAAT
SfcI 307 CTGCAG C^T[GA][CT]AG
520 CTACAG
SspBI 362 TGTACA T^GTACA
502 TGTACA
TaqI 350 TCGA T^CGA
705 TCGA
TthHB8I 350 TCGA T^CGA
705 TCGA
Van91I 817 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2282 CCAGATGTTGG
XhoII 989 GGATCC [GA]^GATC[CT]
2150 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AvaII BbvCI BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI