################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AclI AflII Alw44I ApaLI AscI AspI AviII BalI BbrPI BcgI BclI BfrI BglII BsaAI BsaBI BsiEI BsiWI BspDI BspHI BsrDI BssSI BstAPI BstBI BstEII BstPI ClaI CpoI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HpaI KspI MaeII MamI MluI MluNI MscI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1253 GGTACC G^GTACC AccIII 726 TCCGGA T^CCGGA AcsI 1387 AAATTC [GA]^AATT[CT] AgeI 534 ACCGGT A^CCGGT ApaI 2319 GGGCCC GGGCC^C ApoI 1387 AAATTC [GA]^AATT[CT] Asp700I 593 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1253 GGTACC G^GTACC AspHI 1916 GAGCAC G[AT]GC[AT]^C AvrII 1671 CCTAGG C^CTAGG BanII 2319 GGGCCC G[GA]GC[CT]^C BbeI 4 GGCGCC GGCGC^C BciVI 409 GGATAC GTATCC(6/5) BglI 37 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1671 CCTAGG C^CTAGG BlpI 1864 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1864 GCTCAGC GC^T[ACGT]AGC BsaI 1063 GAGACC GGTCTC(1/5) BseAI 726 TCCGGA T^CCGGA BsiHKAI 1916 GAGCAC G[AT]GC[AT]^C BsmBI 890 GAGACG CGTCTC(1/5) BspEI 726 TCCGGA T^CCGGA BspMI 1624 ACCTGC ACCTGC(4/8) BssHII 11 GCGCGC G^CGCGC Bst1107I 579 GTATAC GTA^TAC BstZ17I 579 GTATAC GTA^TAC CelII 1864 GCTCAGC GC^T[ACGT]AGC EcoRV 2171 GATATC GAT^ATC HindIII 2587 AAGCTT A^AGCTT KasI 4 GGCGCC G^GCGCC KpnI 1253 GGTACC GGTAC^C MfeI 1043 CAATTG C^AATTG MroI 726 TCCGGA T^CCGGA MunI 1043 CAATTG C^AATTG NaeI 109 GCCGGC GCC^GGC NarI 4 GGCGCC GG^CGCC NgoMIV 109 GCCGGC G^CCGGC NheI 1860 GCTAGC G^CTAGC PinAI 534 ACCGGT A^CCGGT PstI 307 CTGCAG CTGCA^G SapI 635 GAAGAGC GCTCTTC(1/4) SexAI 266 ACCTGGT A^CC[AT]GGT SfiI 36 GGCCGCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 4 GGCGCC GGC^GCC SmaI 76 CCCGGG CCC^GGG SmlI 667 CTCAAG C^T[CT][GA]AG SspI 2298 AATATT AAT^ATT StuI 921 AGGCCT AGG^CCT SwaI 2225 ATTTAAAT ATTT^AAAT TfiI 2641 GATTC G^A[AT]TC XmaCI 76 CCCGGG C^CCGGG XmaI 76 CCCGGG C^CCGGG XmnI 593 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccI 579 GTATAC GT^[AC][GT]AC 1700 GTCTAC AcyI 4 GGCGCC G[GA]^CG[CT]C 166 GACGCC AhdI 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1114 GACCTCCTGTC AocI 741 CCTCAGG CC^T[ACGT]AGG 1751 CCTGAGG Aor51HI 335 AGCGCT AGC^GCT 1094 AGCGCT AseI 1402 ATTAAT AT^TAAT 1692 ATTAAT AsnI 1402 ATTAAT AT^TAAT 1692 ATTAAT AspEI 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1114 GACCTCCTGTC AvaI 32 CTCGGG C^[CT]CG[GA]G 76 CCCGGG BamHI 989 GGATCC G^GATCC 2150 GGATCC BanI 4 GGCGCC G^G[CT][GA]CC 1253 GGTACC BbsI 1572 GAAGAC GAAGAC(2/6) 2426 GTCTTC BcnI 76 CCCGG CC^[GC]GG 77 CCGGG BpuAI 1572 GAAGAC GAAGAC(2/6) 2426 GTCTTC BsaHI 4 GGCGCC G[GA]^CG[CT]C 166 GACGCC BsaWI 534 ACCGGT [AT]^CCGG[AT] 726 TCCGGA BsgI 1179 GTGCAG GTGCAG(16/14) 1790 GTGCAG BsmI 907 GAATGC GAATGC(1/-1) 1801 GCATTC BsoBI 32 CTCGGG C^[CT]CG[GA]G 76 CCCGGG Bsp1407I 362 TGTACA T^GTACA 502 TGTACA BsrFI 109 GCCGGC [GA]^CCGG[CT] 534 ACCGGT BsrGI 362 TGTACA T^GTACA 502 TGTACA BstXI 260 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 483 CCAGGCAACTGG BstYI 989 GGATCC [GA]^GATC[CT] 2150 GGATCC Bsu36I 741 CCTCAGG CC^T[ACGT]AGG 1751 CCTGAGG Cfr10I 109 GCCGGC [GA]^CCGG[CT] 534 ACCGGT DsaI 191 CCGTGG C^C[GA][CT]GG 205 CCGTGG EaeI 182 TGGCCG [CT]^GGCC[GA] 2113 CGGCCA Eam1105I 533 GACCGGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1114 GACCTCCTGTC Eco47III 335 AGCGCT AGC^GCT 1094 AGCGCT Eco81I 741 CCTCAGG CC^T[ACGT]AGG 1751 CCTGAGG EcoNI 1780 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2362 CCTCTCTCAGG Hin1I 4 GGCGCC G[GA]^CG[CT]C 166 GACGCC MflI 989 GGATCC [GA]^GATC[CT] 2150 GGATCC NciI 76 CCCGG CC^[GC]GG 77 CCGGG PflMI 817 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2282 CCAGATGTTGG PshBI 1402 ATTAAT AT^TAAT 1692 ATTAAT SfcI 307 CTGCAG C^T[GA][CT]AG 520 CTACAG SspBI 362 TGTACA T^GTACA 502 TGTACA TaqI 350 TCGA T^CGA 705 TCGA TthHB8I 350 TCGA T^CGA 705 TCGA Van91I 817 CCAAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2282 CCAGATGTTGG XhoII 989 GGATCC [GA]^GATC[CT] 2150 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AvaII BbvCI BbvI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI