##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AclI        AflII       Alw44I      ApaLI       
AscI        AspI        AviII       BalI        BbrPI       
BcgI        BclI        BfrI        BglII       BsaAI       
BsaBI       BsiEI       BsiWI       BspDI       BspHI       
BsrDI       BssSI       BstAPI      BstBI       BstEII      
BstPI       ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco52I      EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KspI        MaeII       MamI        
MluI        MluNI       MscI        NcoI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1253  GGTACC               G^GTACC
AccIII        726  TCCGGA               T^CCGGA
AcsI         1387  AAATTC               [GA]^AATT[CT]
AgeI          534  ACCGGT               A^CCGGT
ApaI         2319  GGGCCC               GGGCC^C
ApoI         1387  AAATTC               [GA]^AATT[CT]
Asp700I       593  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1253  GGTACC               G^GTACC
AspHI        1916  GAGCAC               G[AT]GC[AT]^C
AvrII        1671  CCTAGG               C^CTAGG
BanII        2319  GGGCCC               G[GA]GC[CT]^C
BbeI            4  GGCGCC               GGCGC^C
BciVI         409  GGATAC               GTATCC(6/5)
BglI           37  GCCGCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1671  CCTAGG               C^CTAGG
BlpI         1864  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1864  GCTCAGC              GC^T[ACGT]AGC
BsaI         1063  GAGACC               GGTCTC(1/5)
BseAI         726  TCCGGA               T^CCGGA
BsiHKAI      1916  GAGCAC               G[AT]GC[AT]^C
BsmBI         890  GAGACG               CGTCTC(1/5)
BspEI         726  TCCGGA               T^CCGGA
BspMI        1624  ACCTGC               ACCTGC(4/8)
BssHII         11  GCGCGC               G^CGCGC
Bst1107I      579  GTATAC               GTA^TAC
BstZ17I       579  GTATAC               GTA^TAC
CelII        1864  GCTCAGC              GC^T[ACGT]AGC
EcoRV        2171  GATATC               GAT^ATC
HindIII      2587  AAGCTT               A^AGCTT
KasI            4  GGCGCC               G^GCGCC
KpnI         1253  GGTACC               GGTAC^C
MfeI         1043  CAATTG               C^AATTG
MroI          726  TCCGGA               T^CCGGA
MunI         1043  CAATTG               C^AATTG
NaeI          109  GCCGGC               GCC^GGC
NarI            4  GGCGCC               GG^CGCC
NgoMIV        109  GCCGGC               G^CCGGC
NheI         1860  GCTAGC               G^CTAGC
PinAI         534  ACCGGT               A^CCGGT
PstI          307  CTGCAG               CTGCA^G
SapI          635  GAAGAGC              GCTCTTC(1/4)
SexAI         266  ACCTGGT              A^CC[AT]GGT
SfiI           36  GGCCGCCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI            4  GGCGCC               GGC^GCC
SmaI           76  CCCGGG               CCC^GGG
SmlI          667  CTCAAG               C^T[CT][GA]AG
SspI         2298  AATATT               AAT^ATT
StuI          921  AGGCCT               AGG^CCT
SwaI         2225  ATTTAAAT             ATTT^AAAT
TfiI         2641  GATTC                G^A[AT]TC
XmaCI          76  CCCGGG               C^CCGGG
XmaI           76  CCCGGG               C^CCGGG
XmnI          593  GAATTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI          579  GTATAC               GT^[AC][GT]AC
             1700  GTCTAC              
AcyI            4  GGCGCC               G[GA]^CG[CT]C
              166  GACGCC              
AhdI          533  GACCGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1114  GACCTCCTGTC         
AocI          741  CCTCAGG              CC^T[ACGT]AGG
             1751  CCTGAGG             
Aor51HI       335  AGCGCT               AGC^GCT
             1094  AGCGCT              
AseI         1402  ATTAAT               AT^TAAT
             1692  ATTAAT              
AsnI         1402  ATTAAT               AT^TAAT
             1692  ATTAAT              
AspEI         533  GACCGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1114  GACCTCCTGTC         
AvaI           32  CTCGGG               C^[CT]CG[GA]G
               76  CCCGGG              
BamHI         989  GGATCC               G^GATCC
             2150  GGATCC              
BanI            4  GGCGCC               G^G[CT][GA]CC
             1253  GGTACC              
BbsI         1572  GAAGAC               GAAGAC(2/6)
             2426  GTCTTC              
BcnI           76  CCCGG                CC^[GC]GG
               77  CCGGG               
BpuAI        1572  GAAGAC               GAAGAC(2/6)
             2426  GTCTTC              
BsaHI           4  GGCGCC               G[GA]^CG[CT]C
              166  GACGCC              
BsaWI         534  ACCGGT               [AT]^CCGG[AT]
              726  TCCGGA              
BsgI         1179  GTGCAG               GTGCAG(16/14)
             1790  GTGCAG              
BsmI          907  GAATGC               GAATGC(1/-1)
             1801  GCATTC              
BsoBI          32  CTCGGG               C^[CT]CG[GA]G
               76  CCCGGG              
Bsp1407I      362  TGTACA               T^GTACA
              502  TGTACA              
BsrFI         109  GCCGGC               [GA]^CCGG[CT]
              534  ACCGGT              
BsrGI         362  TGTACA               T^GTACA
              502  TGTACA              
BstXI         260  CCAACAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              483  CCAGGCAACTGG        
BstYI         989  GGATCC               [GA]^GATC[CT]
             2150  GGATCC              
Bsu36I        741  CCTCAGG              CC^T[ACGT]AGG
             1751  CCTGAGG             
Cfr10I        109  GCCGGC               [GA]^CCGG[CT]
              534  ACCGGT              
DsaI          191  CCGTGG               C^C[GA][CT]GG
              205  CCGTGG              
EaeI          182  TGGCCG               [CT]^GGCC[GA]
             2113  CGGCCA              
Eam1105I      533  GACCGGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1114  GACCTCCTGTC         
Eco47III      335  AGCGCT               AGC^GCT
             1094  AGCGCT              
Eco81I        741  CCTCAGG              CC^T[ACGT]AGG
             1751  CCTGAGG             
EcoNI        1780  CCTGGCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2362  CCTCTCTCAGG         
Hin1I           4  GGCGCC               G[GA]^CG[CT]C
              166  GACGCC              
MflI          989  GGATCC               [GA]^GATC[CT]
             2150  GGATCC              
NciI           76  CCCGG                CC^[GC]GG
               77  CCGGG               
PflMI         817  CCAAGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2282  CCAGATGTTGG         
PshBI        1402  ATTAAT               AT^TAAT
             1692  ATTAAT              
SfcI          307  CTGCAG               C^T[GA][CT]AG
              520  CTACAG              
SspBI         362  TGTACA               T^GTACA
              502  TGTACA              
TaqI          350  TCGA                 T^CGA
              705  TCGA                
TthHB8I       350  TCGA                 T^CGA
              705  TCGA                
Van91I        817  CCAAGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2282  CCAGATGTTGG         
XhoII         989  GGATCC               [GA]^GATC[CT]
             2150  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AvaII       BbvCI       BbvI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspLU11I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XcmI