################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BclI BfrI BlnI BsaBI BsiWI BsmBI BspLU11I BssSI BstAPI BstEII BstPI Bsu36I CpoI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII KpnI MamI MluI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SbfI SexAI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccIII 2605 TCCGGA T^CCGGA AclI 3093 AACGTT AA^CGTT Alw44I 405 GTGCAC G^TGCAC Aor51HI 588 AGCGCT AGC^GCT ApaLI 405 GTGCAC G^TGCAC BcgI 487 GCATTTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 62 GCTAAGC GC^T[ACGT]AGC Bpu1102I 62 GCTAAGC GC^T[ACGT]AGC BsaAI 536 TACGTG [CT]AC^GT[GA] BsaI 2769 GAGACC GGTCTC(1/5) BseAI 2605 TCCGGA T^CCGGA BsiEI 74 CGGCCG CG[GA][CT]^CG BsmI 2873 GAATGC GAATGC(1/-1) Bsp1407I 911 TGTACA T^GTACA BspDI 1971 ATCGAT AT^CGAT BspEI 2605 TCCGGA T^CCGGA BspHI 2321 TCATGA T^CATGA BspMI 2621 GCAGGT ACCTGC(4/8) BsrDI 2984 CATTGC GCAATG(2/0) BsrFI 120 GCCGGC [GA]^CCGG[CT] BsrGI 911 TGTACA T^GTACA BssHII 391 GCGCGC G^CGCGC Bst1107I 3024 GTATAC GTA^TAC BstBI 2527 TTCGAA TT^CGAA BstXI 2258 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3024 GTATAC GTA^TAC CelII 62 GCTAAGC GC^T[ACGT]AGC Cfr10I 120 GCCGGC [GA]^CCGG[CT] ClaI 1971 ATCGAT AT^CGAT DraIII 865 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 74 CGGCCG C^GGCCG EclXI 74 CGGCCG C^GGCCG Eco47III 588 AGCGCT AGC^GCT Eco52I 74 CGGCCG C^GGCCG EcoRI 696 GAATTC G^AATTC HgaI 176 GCGTC GACGC(5/10) HpaI 1269 GTTAAC GTT^AAC MfeI 2143 CAATTG C^AATTG MroI 2605 TCCGGA T^CCGGA MunI 2143 CAATTG C^AATTG NaeI 120 GCCGGC GCC^GGC NgoMIV 120 GCCGGC G^CCGGC NspI 2551 ACATGC [GA]CATG^[CT] NspV 2527 TTCGAA TT^CGAA PflMI 2837 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 3093 AACGTT AA^CGTT PstI 2764 CTGCAG CTGCA^G RcaI 2321 TCATGA T^CATGA SacI 1476 GAGCTC GAGCT^C SalI 1668 GTCGAC G^TCGAC SapI 518 GAAGAGC GCTCTTC(1/4) ScaI 3113 AGTACT AGT^ACT SfiI 119 GGCCGGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2527 TTCGAA TT^CGAA SspBI 911 TGTACA T^GTACA SspI 2270 AATATT AAT^ATT StuI 2536 AGGCCT AGG^CCT Van91I 2837 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 924 TCTAGA T^CTAGA XcmI 2939 CCAACCGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1668 GTCGAC GT^[AC][GT]AC 3024 GTATAC AflIII 537 ACGTGT A^C[GA][CT]GT 2452 ACACGT AlwNI 1933 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 2838 CAGATCCTG AspI 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2402 GACCGAGTC BamHI 2219 GGATCC G^GATCC 2656 GGATCC BanII 1476 GAGCTC G[GA]GC[CT]^C 2915 GAGCCC BbsI 1395 GAAGAC GAAGAC(2/6) 978 GTCTTC BciVI 533 GGATAC GTATCC(6/5) 1424 GTATCC BpuAI 1395 GAAGAC GAAGAC(2/6) 978 GTCTTC BsaWI 1010 TCCGGT [AT]^CCGG[AT] 2605 TCCGGA BsgI 2340 CTGCAC GTGCAG(16/14) 152 GTGCAG BsrBI 142 GAGCGG CCGCTC(-3/-3) 282 GAGCGG DraI 1278 TTTAAA TTT^AAA 3183 TTTAAA HincII 1269 GTTAAC GT[CT]^[GA]AC 1668 GTCGAC HindII 1269 GTTAAC GT[CT]^[GA]AC 1668 GTCGAC HphI 1013 GGTGA GGTGA(8/7) 990 TCACC NcoI 220 CCATGG C^CATGG 1285 CCATGG PflFI 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2402 GACCGAGTC PpuMI 939 AGGACCC [GA]G^G[AT]CC[CT] 1956 GGGTCCT PvuII 1165 CAGCTG CAG^CTG 1920 CAGCTG SmaI 202 CCCGGG CCC^GGG 229 CCCGGG SmlI 1655 CTCAAG C^T[CT][GA]AG 3156 CTTGAG Tth111I 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2402 GACCGAGTC XmaCI 202 CCCGGG C^CCGGG 229 CCCGGG XmaI 202 CCCGGG C^CCGGG 229 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BbeI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI