##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BclI BfrI BlnI BsaBI BsiWI
BsmBI BspLU11I BssSI BstAPI BstEII
BstPI Bsu36I CpoI DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
MamI MluI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SbfI SexAI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 2605 TCCGGA T^CCGGA
AclI 3093 AACGTT AA^CGTT
Alw44I 405 GTGCAC G^TGCAC
Aor51HI 588 AGCGCT AGC^GCT
ApaLI 405 GTGCAC G^TGCAC
BcgI 487 GCATTTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 62 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 62 GCTAAGC GC^T[ACGT]AGC
BsaAI 536 TACGTG [CT]AC^GT[GA]
BsaI 2769 GAGACC GGTCTC(1/5)
BseAI 2605 TCCGGA T^CCGGA
BsiEI 74 CGGCCG CG[GA][CT]^CG
BsmI 2873 GAATGC GAATGC(1/-1)
Bsp1407I 911 TGTACA T^GTACA
BspDI 1971 ATCGAT AT^CGAT
BspEI 2605 TCCGGA T^CCGGA
BspHI 2321 TCATGA T^CATGA
BspMI 2621 GCAGGT ACCTGC(4/8)
BsrDI 2984 CATTGC GCAATG(2/0)
BsrFI 120 GCCGGC [GA]^CCGG[CT]
BsrGI 911 TGTACA T^GTACA
BssHII 391 GCGCGC G^CGCGC
Bst1107I 3024 GTATAC GTA^TAC
BstBI 2527 TTCGAA TT^CGAA
BstXI 2258 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3024 GTATAC GTA^TAC
CelII 62 GCTAAGC GC^T[ACGT]AGC
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
ClaI 1971 ATCGAT AT^CGAT
DraIII 865 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 74 CGGCCG C^GGCCG
EclXI 74 CGGCCG C^GGCCG
Eco47III 588 AGCGCT AGC^GCT
Eco52I 74 CGGCCG C^GGCCG
EcoRI 696 GAATTC G^AATTC
HgaI 176 GCGTC GACGC(5/10)
HpaI 1269 GTTAAC GTT^AAC
MfeI 2143 CAATTG C^AATTG
MroI 2605 TCCGGA T^CCGGA
MunI 2143 CAATTG C^AATTG
NaeI 120 GCCGGC GCC^GGC
NgoMIV 120 GCCGGC G^CCGGC
NspI 2551 ACATGC [GA]CATG^[CT]
NspV 2527 TTCGAA TT^CGAA
PflMI 2837 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3093 AACGTT AA^CGTT
PstI 2764 CTGCAG CTGCA^G
RcaI 2321 TCATGA T^CATGA
SacI 1476 GAGCTC GAGCT^C
SalI 1668 GTCGAC G^TCGAC
SapI 518 GAAGAGC GCTCTTC(1/4)
ScaI 3113 AGTACT AGT^ACT
SfiI 119 GGCCGGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2527 TTCGAA TT^CGAA
SspBI 911 TGTACA T^GTACA
SspI 2270 AATATT AAT^ATT
StuI 2536 AGGCCT AGG^CCT
Van91I 2837 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 924 TCTAGA T^CTAGA
XcmI 2939 CCAACCGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1668 GTCGAC GT^[AC][GT]AC
3024 GTATAC
AflIII 537 ACGTGT A^C[GA][CT]GT
2452 ACACGT
AlwNI 1933 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
2838 CAGATCCTG
AspI 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2402 GACCGAGTC
BamHI 2219 GGATCC G^GATCC
2656 GGATCC
BanII 1476 GAGCTC G[GA]GC[CT]^C
2915 GAGCCC
BbsI 1395 GAAGAC GAAGAC(2/6)
978 GTCTTC
BciVI 533 GGATAC GTATCC(6/5)
1424 GTATCC
BpuAI 1395 GAAGAC GAAGAC(2/6)
978 GTCTTC
BsaWI 1010 TCCGGT [AT]^CCGG[AT]
2605 TCCGGA
BsgI 2340 CTGCAC GTGCAG(16/14)
152 GTGCAG
BsrBI 142 GAGCGG CCGCTC(-3/-3)
282 GAGCGG
DraI 1278 TTTAAA TTT^AAA
3183 TTTAAA
HincII 1269 GTTAAC GT[CT]^[GA]AC
1668 GTCGAC
HindII 1269 GTTAAC GT[CT]^[GA]AC
1668 GTCGAC
HphI 1013 GGTGA GGTGA(8/7)
990 TCACC
NcoI 220 CCATGG C^CATGG
1285 CCATGG
PflFI 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2402 GACCGAGTC
PpuMI 939 AGGACCC [GA]G^G[AT]CC[CT]
1956 GGGTCCT
PvuII 1165 CAGCTG CAG^CTG
1920 CAGCTG
SmaI 202 CCCGGG CCC^GGG
229 CCCGGG
SmlI 1655 CTCAAG C^T[CT][GA]AG
3156 CTTGAG
Tth111I 972 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2402 GACCGAGTC
XmaCI 202 CCCGGG C^CCGGG
229 CCCGGG
XmaI 202 CCCGGG C^CCGGG
229 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BbeI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI