##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbvCI
BcgI BclI BglI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eco47III EcoO65I EcoRV FbaI
FseI FspI HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 143 GTCGAC GT^[AC][GT]AC
AcyI 1256 GGCGTC G[GA]^CG[CT]C
AflII 289 CTTAAG C^TTAAG
Alw44I 2503 GTGCAC G^TGCAC
ApaI 2632 GGGCCC GGGCC^C
ApaLI 2503 GTGCAC G^TGCAC
Asp700I 657 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2343 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 794 GGATCC G^GATCC
BbrPI 2506 CACGTG CAC^GTG
BciVI 754 GTATCC GTATCC(6/5)
BfrI 289 CTTAAG C^TTAAG
BlpI 865 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 865 GCTCAGC GC^T[ACGT]AGC
BsaAI 2506 CACGTG [CT]AC^GT[GA]
BsaHI 1256 GGCGTC G[GA]^CG[CT]C
BsaI 229 GAGACC GGTCTC(1/5)
BspHI 1616 TCATGA T^CATGA
BsrBI 2161 GAGCGG CCGCTC(-3/-3)
BsrFI 193 ACCGGC [GA]^CCGG[CT]
BstXI 1925 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 865 GCTCAGC GC^T[ACGT]AGC
Cfr10I 193 ACCGGC [GA]^CCGG[CT]
EcoRI 272 GAATTC G^AATTC
FauI 67 CCCGC CCCGC(4/6)
HaeII 63 AGCGCC [GA]GCGC^[CT]
Hin1I 1256 GGCGTC G[GA]^CG[CT]C
NcoI 1371 CCATGG C^CATGG
NspI 1928 ACATGC [GA]CATG^[CT]
PflFI 2343 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2506 CACGTG CAC^GTG
PmlI 2506 CACGTG CAC^GTG
PpuMI 1997 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1561 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1616 TCATGA T^CATGA
SacI 1501 GAGCTC GAGCT^C
SalI 143 GTCGAC G^TCGAC
SapI 922 GCTCTTC GCTCTTC(1/4)
ScaI 1469 AGTACT AGT^ACT
TfiI 1850 GATTC G^A[AT]TC
Tth111I 2343 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 809 CCATTTCAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 657 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 117 CGCG CG^CG
149 CGCG
AocI 1534 CCTGAGG CC^T[ACGT]AGG
2827 CCTCAGG
BanI 2037 GGTGCC G^G[CT][GA]CC
2685 GGCACC
Bsp1407I 563 TGTACA T^GTACA
1211 TGTACA
BsrGI 563 TGTACA T^GTACA
1211 TGTACA
BstAPI 2268 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3031 GCATGGAGTGC
BstUI 117 CGCG CG^CG
149 CGCG
BstYI 794 GGATCC [GA]^GATC[CT]
1418 AGATCC
Bsu36I 1534 CCTGAGG CC^T[ACGT]AGG
2827 CCTCAGG
EagI 151 CGGCCG C^GGCCG
195 CGGCCG
EclXI 151 CGGCCG C^GGCCG
195 CGGCCG
Eco52I 151 CGGCCG C^GGCCG
195 CGGCCG
Eco81I 1534 CCTGAGG CC^T[ACGT]AGG
2827 CCTCAGG
EcoT22I 1198 ATGCAT ATGCA^T
1838 ATGCAT
HincII 143 GTCGAC GT[CT]^[GA]AC
987 GTCAAC
HindII 143 GTCGAC GT[CT]^[GA]AC
987 GTCAAC
KspI 116 CCGCGG CCGC^GG
148 CCGCGG
MflI 794 GGATCC [GA]^GATC[CT]
1418 AGATCC
MvnI 117 CGCG CG^CG
149 CGCG
NsiI 1198 ATGCAT ATGCA^T
1838 ATGCAT
PflMI 2636 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3019 CCATCCCTTGG
SacII 116 CCGCGG CCGC^GG
148 CCGCGG
SexAI 1268 ACCAGGT A^CC[AT]GGT
1973 ACCAGGT
SspBI 563 TGTACA T^GTACA
1211 TGTACA
Van91I 2636 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3019 CCATCCCTTGG
XhoII 794 GGATCC [GA]^GATC[CT]
1418 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AspHI
AvaI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI