##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BbrPI BbsI BcgI
BciVI BclI BfrI BglII BpuAI
BsaBI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NsiI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 1357 GAATTT [GA]^AATT[CT]
ApoI 1357 GAATTT [GA]^AATT[CT]
AspHI 1205 GTGCTC G[AT]GC[AT]^C
AspI 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1463 CCTAGG C^CTAGG
BalI 837 TGGCCA TGG^CCA
BamHI 136 GGATCC G^GATCC
BbeI 38 GGCGCC GGCGC^C
BlnI 1463 CCTAGG C^CTAGG
BsaAI 330 TACGTG [CT]AC^GT[GA]
BsaWI 1337 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1205 GTGCTC G[AT]GC[AT]^C
BsrDI 7 CATTGC GCAATG(2/0)
BsrFI 126 GCCGGT [GA]^CCGG[CT]
BssSI 13 CTCGTG CACGAG(-5/-1)
BstBI 1789 TTCGAA TT^CGAA
Cfr10I 126 GCCGGT [GA]^CCGG[CT]
KasI 38 GGCGCC G^GCGCC
MluNI 837 TGGCCA TGG^CCA
MscI 837 TGGCCA TGG^CCA
NarI 38 GGCGCC GG^CGCC
NheI 1535 GCTAGC G^CTAGC
NruI 160 TCGCGA TCG^CGA
NspI 807 ACATGC [GA]CATG^[CT]
NspV 1789 TTCGAA TT^CGAA
PacI 2911 TTAATTAA TTAAT^TAA
PflFI 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2013 CAGCTG CAG^CTG
SapI 2580 GAAGAGC GCTCTTC(1/4)
SexAI 1635 ACCTGGT A^CC[AT]GGT
SfoI 38 GGCGCC GGC^GCC
SfuI 1789 TTCGAA TT^CGAA
SmaI 339 CCCGGG CCC^GGG
SmlI 2173 CTCAAG C^T[CT][GA]AG
Tth111I 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 383 TCTAGA T^CTAGA
XmaCI 339 CCCGGG C^CCGGG
XmaI 339 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1719 GTCTAC GT^[AC][GT]AC
2547 GTAGAC
AcyI 38 GGCGCC G[GA]^CG[CT]C
923 GACGCC
Aor51HI 680 AGCGCT AGC^GCT
852 AGCGCT
BbvCI 1273 CCTCAGC CCTCAGC(-5/-2)
1385 CCTCAGC
BlpI 1943 GCTCAGC GC^T[ACGT]AGC
2778 GCTGAGC
Bpu1102I 1943 GCTCAGC GC^T[ACGT]AGC
2778 GCTGAGC
BsaHI 38 GGCGCC G[GA]^CG[CT]C
923 GACGCC
BsmBI 1043 GAGACG CGTCTC(1/5)
2527 GAGACG
BsmI 1889 GCATTC GAATGC(1/-1)
2585 GCATTC
Bsp1407I 456 TGTACA T^GTACA
1585 TGTACA
BsrGI 456 TGTACA T^GTACA
1585 TGTACA
BstAPI 1439 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2398 GCACCTCATGC
CelII 1943 GCTCAGC GC^T[ACGT]AGC
2778 GCTGAGC
Eco47III 680 AGCGCT AGC^GCT
852 AGCGCT
EcoNI 1478 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2768 CCTGCCAGAGG
Hin1I 38 GGCGCC G[GA]^CG[CT]C
923 GACGCC
MspA1I 83 CAGCGG C[AC]G^C[GT]G
2013 CAGCTG
PflMI 2429 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2537 CCACTTGATGG
PpuMI 342 GGGACCT [GA]G^G[AT]CC[CT]
2854 GGGTCCC
PstI 1650 CTGCAG CTGCA^G
2300 CTGCAG
SspBI 456 TGTACA T^GTACA
1585 TGTACA
Van91I 2429 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2537 CCACTTGATGG
XcmI 825 CCATGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1483 CCAAGGGCTCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII