################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbrPI BbsI BcgI BciVI BclI BfrI BglII BpuAI BsaBI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NsiI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 1357 GAATTT [GA]^AATT[CT] ApoI 1357 GAATTT [GA]^AATT[CT] AspHI 1205 GTGCTC G[AT]GC[AT]^C AspI 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1463 CCTAGG C^CTAGG BalI 837 TGGCCA TGG^CCA BamHI 136 GGATCC G^GATCC BbeI 38 GGCGCC GGCGC^C BlnI 1463 CCTAGG C^CTAGG BsaAI 330 TACGTG [CT]AC^GT[GA] BsaWI 1337 ACCGGA [AT]^CCGG[AT] BsiHKAI 1205 GTGCTC G[AT]GC[AT]^C BsrDI 7 CATTGC GCAATG(2/0) BsrFI 126 GCCGGT [GA]^CCGG[CT] BssSI 13 CTCGTG CACGAG(-5/-1) BstBI 1789 TTCGAA TT^CGAA Cfr10I 126 GCCGGT [GA]^CCGG[CT] KasI 38 GGCGCC G^GCGCC MluNI 837 TGGCCA TGG^CCA MscI 837 TGGCCA TGG^CCA NarI 38 GGCGCC GG^CGCC NheI 1535 GCTAGC G^CTAGC NruI 160 TCGCGA TCG^CGA NspI 807 ACATGC [GA]CATG^[CT] NspV 1789 TTCGAA TT^CGAA PacI 2911 TTAATTAA TTAAT^TAA PflFI 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 2013 CAGCTG CAG^CTG SapI 2580 GAAGAGC GCTCTTC(1/4) SexAI 1635 ACCTGGT A^CC[AT]GGT SfoI 38 GGCGCC GGC^GCC SfuI 1789 TTCGAA TT^CGAA SmaI 339 CCCGGG CCC^GGG SmlI 2173 CTCAAG C^T[CT][GA]AG Tth111I 2130 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 383 TCTAGA T^CTAGA XmaCI 339 CCCGGG C^CCGGG XmaI 339 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 1719 GTCTAC GT^[AC][GT]AC 2547 GTAGAC AcyI 38 GGCGCC G[GA]^CG[CT]C 923 GACGCC Aor51HI 680 AGCGCT AGC^GCT 852 AGCGCT BbvCI 1273 CCTCAGC CCTCAGC(-5/-2) 1385 CCTCAGC BlpI 1943 GCTCAGC GC^T[ACGT]AGC 2778 GCTGAGC Bpu1102I 1943 GCTCAGC GC^T[ACGT]AGC 2778 GCTGAGC BsaHI 38 GGCGCC G[GA]^CG[CT]C 923 GACGCC BsmBI 1043 GAGACG CGTCTC(1/5) 2527 GAGACG BsmI 1889 GCATTC GAATGC(1/-1) 2585 GCATTC Bsp1407I 456 TGTACA T^GTACA 1585 TGTACA BsrGI 456 TGTACA T^GTACA 1585 TGTACA BstAPI 1439 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2398 GCACCTCATGC CelII 1943 GCTCAGC GC^T[ACGT]AGC 2778 GCTGAGC Eco47III 680 AGCGCT AGC^GCT 852 AGCGCT EcoNI 1478 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2768 CCTGCCAGAGG Hin1I 38 GGCGCC G[GA]^CG[CT]C 923 GACGCC MspA1I 83 CAGCGG C[AC]G^C[GT]G 2013 CAGCTG PflMI 2429 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2537 CCACTTGATGG PpuMI 342 GGGACCT [GA]G^G[AT]CC[CT] 2854 GGGTCCC PstI 1650 CTGCAG CTGCA^G 2300 CTGCAG SspBI 456 TGTACA T^GTACA 1585 TGTACA Van91I 2429 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2537 CCACTTGATGG XcmI 825 CCATGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1483 CCAAGGGCTCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII