##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AspEI AviII
BamHI BbeI BcgI BfrI BglI
BglII BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI BstEII
BstPI BstUI Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FseI FspI HgaI
Hin1I HindIII HpaI KasI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 860 GGTACC G^GTACC
AciI 2157 GCGG CCGC(-3/-1)
AclI 2544 AACGTT AA^CGTT
Aor51HI 1942 AGCGCT AGC^GCT
AseI 2881 ATTAAT AT^TAAT
AsnI 2881 ATTAAT AT^TAAT
Asp718I 860 GGTACC G^GTACC
AspHI 2407 GAGCAC G[AT]GC[AT]^C
AvaI 739 CCCGAG C^[CT]CG[GA]G
AvrII 1356 CCTAGG C^CTAGG
BalI 912 TGGCCA TGG^CCA
BanII 826 GGGCTC G[GA]GC[CT]^C
BbvCI 1144 GCTGAGG CCTCAGC(-5/-2)
BciVI 2621 GTATCC GTATCC(6/5)
BclI 1812 TGATCA T^GATCA
BcnI 466 CCCGG CC^[GC]GG
BlnI 1356 CCTAGG C^CTAGG
BlpI 30 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTTAGC GC^T[ACGT]AGC
BsaBI 685 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2407 GAGCAC G[AT]GC[AT]^C
BsmBI 2391 GAGACG CGTCTC(1/5)
BsoBI 739 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2985 TGTACA T^GTACA
BspHI 2057 TCATGA T^CATGA
BspMI 2975 ACCTGC ACCTGC(4/8)
BsrGI 2985 TGTACA T^GTACA
Bst1107I 3028 GTATAC GTA^TAC
BstAPI 2265 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2561 TTCGAA TT^CGAA
BstZ17I 3028 GTATAC GTA^TAC
CelII 30 GCTTAGC GC^T[ACGT]AGC
CfoI 1943 GCGC GCG^C
DraI 2470 TTTAAA TTT^AAA
Eco47III 1942 AGCGCT AGC^GCT
EcoRI 2851 GAATTC G^AATTC
EcoT22I 2872 ATGCAT ATGCA^T
FbaI 1812 TGATCA T^GATCA
HaeII 1942 AGCGCT [GA]GCGC^[CT]
HapII 467 CCGG C^CGG
HhaI 1943 GCGC GCG^C
HinP1I 1943 GCGC G^CGC
HpaII 467 CCGG C^CGG
KpnI 860 GGTACC GGTAC^C
MamI 685 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 528 CAATTG C^AATTG
MluNI 912 TGGCCA TGG^CCA
MscI 912 TGGCCA TGG^CCA
MspI 467 CCGG C^CGG
MunI 528 CAATTG C^AATTG
NciI 466 CCCGG CC^[GC]GG
NsiI 2872 ATGCAT ATGCA^T
NspV 2561 TTCGAA TT^CGAA
PshBI 2881 ATTAAT AT^TAAT
Psp1406I 2544 AACGTT AA^CGTT
PstI 1460 CTGCAG CTGCA^G
PvuII 696 CAGCTG CAG^CTG
RcaI 2057 TCATGA T^CATGA
SfuI 2561 TTCGAA TT^CGAA
SspBI 2985 TGTACA T^GTACA
SspI 646 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspI 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACTAAGTC
BbrPI 2923 CACGTG CAC^GTG
3121 CACGTG
BmyI 826 GGGCTC G[AGT]GC[ACT]^C
2407 GAGCAC
BsaAI 2923 CACGTG [CT]AC^GT[GA]
3121 CACGTG
BseRI 1500 CTCCTC GAGGAG(10/8)
1147 GAGGAG
BsmI 894 GCATTC GAATGC(1/-1)
1433 GCATTC
Bsp1286I 826 GGGCTC G[AGT]GC[ACT]^C
2407 GAGCAC
BsrDI 1137 CATTGC GCAATG(2/0)
2963 GCAATG
EaeI 912 TGGCCA [CT]^GGCC[GA]
2997 CGGCCA
MspA1I 696 CAGCTG C[AC]G^C[GT]G
2155 CAGCGG
NcoI 2508 CCATGG C^CATGG
2626 CCATGG
PflFI 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACTAAGTC
PflMI 505 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1922 CCATCGTGTGG
PmaCI 2923 CACGTG CAC^GTG
3121 CACGTG
PmlI 2923 CACGTG CAC^GTG
3121 CACGTG
SphI 492 GCATGC GCATG^C
2874 GCATGC
StuI 1058 AGGCCT AGG^CCT
2910 AGGCCT
Tth111I 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3186 GACTAAGTC
Van91I 505 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1922 CCATCGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AvaII BanI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI