################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AspEI AviII BamHI BbeI BcgI BfrI BglI BglII BsaHI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI BstEII BstPI BstUI Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HgaI Hin1I HindIII HpaI KasI KspI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 860 GGTACC G^GTACC AciI 2157 GCGG CCGC(-3/-1) AclI 2544 AACGTT AA^CGTT Aor51HI 1942 AGCGCT AGC^GCT AseI 2881 ATTAAT AT^TAAT AsnI 2881 ATTAAT AT^TAAT Asp718I 860 GGTACC G^GTACC AspHI 2407 GAGCAC G[AT]GC[AT]^C AvaI 739 CCCGAG C^[CT]CG[GA]G AvrII 1356 CCTAGG C^CTAGG BalI 912 TGGCCA TGG^CCA BanII 826 GGGCTC G[GA]GC[CT]^C BbvCI 1144 GCTGAGG CCTCAGC(-5/-2) BciVI 2621 GTATCC GTATCC(6/5) BclI 1812 TGATCA T^GATCA BcnI 466 CCCGG CC^[GC]GG BlnI 1356 CCTAGG C^CTAGG BlpI 30 GCTTAGC GC^T[ACGT]AGC Bpu1102I 30 GCTTAGC GC^T[ACGT]AGC BsaBI 685 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2407 GAGCAC G[AT]GC[AT]^C BsmBI 2391 GAGACG CGTCTC(1/5) BsoBI 739 CCCGAG C^[CT]CG[GA]G Bsp1407I 2985 TGTACA T^GTACA BspHI 2057 TCATGA T^CATGA BspMI 2975 ACCTGC ACCTGC(4/8) BsrGI 2985 TGTACA T^GTACA Bst1107I 3028 GTATAC GTA^TAC BstAPI 2265 GCATCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2561 TTCGAA TT^CGAA BstZ17I 3028 GTATAC GTA^TAC CelII 30 GCTTAGC GC^T[ACGT]AGC CfoI 1943 GCGC GCG^C DraI 2470 TTTAAA TTT^AAA Eco47III 1942 AGCGCT AGC^GCT EcoRI 2851 GAATTC G^AATTC EcoT22I 2872 ATGCAT ATGCA^T FbaI 1812 TGATCA T^GATCA HaeII 1942 AGCGCT [GA]GCGC^[CT] HapII 467 CCGG C^CGG HhaI 1943 GCGC GCG^C HinP1I 1943 GCGC G^CGC HpaII 467 CCGG C^CGG KpnI 860 GGTACC GGTAC^C MamI 685 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 528 CAATTG C^AATTG MluNI 912 TGGCCA TGG^CCA MscI 912 TGGCCA TGG^CCA MspI 467 CCGG C^CGG MunI 528 CAATTG C^AATTG NciI 466 CCCGG CC^[GC]GG NsiI 2872 ATGCAT ATGCA^T NspV 2561 TTCGAA TT^CGAA PshBI 2881 ATTAAT AT^TAAT Psp1406I 2544 AACGTT AA^CGTT PstI 1460 CTGCAG CTGCA^G PvuII 696 CAGCTG CAG^CTG RcaI 2057 TCATGA T^CATGA SfuI 2561 TTCGAA TT^CGAA SspBI 2985 TGTACA T^GTACA SspI 646 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspI 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3186 GACTAAGTC BbrPI 2923 CACGTG CAC^GTG 3121 CACGTG BmyI 826 GGGCTC G[AGT]GC[ACT]^C 2407 GAGCAC BsaAI 2923 CACGTG [CT]AC^GT[GA] 3121 CACGTG BseRI 1500 CTCCTC GAGGAG(10/8) 1147 GAGGAG BsmI 894 GCATTC GAATGC(1/-1) 1433 GCATTC Bsp1286I 826 GGGCTC G[AGT]GC[ACT]^C 2407 GAGCAC BsrDI 1137 CATTGC GCAATG(2/0) 2963 GCAATG EaeI 912 TGGCCA [CT]^GGCC[GA] 2997 CGGCCA MspA1I 696 CAGCTG C[AC]G^C[GT]G 2155 CAGCGG NcoI 2508 CCATGG C^CATGG 2626 CCATGG PflFI 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3186 GACTAAGTC PflMI 505 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1922 CCATCGTGTGG PmaCI 2923 CACGTG CAC^GTG 3121 CACGTG PmlI 2923 CACGTG CAC^GTG 3121 CACGTG SphI 492 GCATGC GCATG^C 2874 GCATGC StuI 1058 AGGCCT AGG^CCT 2910 AGGCCT Tth111I 3130 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3186 GACTAAGTC Van91I 505 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1922 CCATCGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanI BbsI BbvI BfaI BpmI BpuAI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI