##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AccIII AclI AcsI AflIII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BcgI BclI BlnI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DrdI Eam1105I Eco47III
EcoNI EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI MfeI
MluI MroI MunI NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 2090 CTTAAG C^TTAAG
AspI 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 895 TGGCCA TGG^CCA
BbrPI 117 CACGTG CAC^GTG
BfrI 2090 CTTAAG C^TTAAG
BglI 888 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1204 AGATCT A^GATCT
BlpI 2720 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2720 GCTGAGC GC^T[ACGT]AGC
BsaAI 117 CACGTG [CT]AC^GT[GA]
BsaBI 1268 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 135 TCCGGT [AT]^CCGG[AT]
BsiEI 226 CGGCCG CG[GA][CT]^CG
Bsp1407I 1726 TGTACA T^GTACA
BsrBI 2390 CCGCTC CCGCTC(-3/-3)
BsrFI 1140 GCCGGC [GA]^CCGG[CT]
BsrGI 1726 TGTACA T^GTACA
BstAPI 2153 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2720 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1140 GCCGGC [GA]^CCGG[CT]
DraI 2757 TTTAAA TTT^AAA
DraIII 1742 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 226 CGGCCG C^GGCCG
EclXI 226 CGGCCG C^GGCCG
Eco52I 226 CGGCCG C^GGCCG
EcoRV 754 GATATC GAT^ATC
MamI 1268 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 895 TGGCCA TGG^CCA
MscI 895 TGGCCA TGG^CCA
NaeI 1140 GCCGGC GCC^GGC
NdeI 1307 CATATG CA^TATG
NgoMIV 1140 GCCGGC G^CCGGC
NotI 225 GCGGCCGC GC^GGCCGC
NspI 2694 GCATGC [GA]CATG^[CT]
PflFI 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1738 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 117 CACGTG CAC^GTG
PmlI 117 CACGTG CAC^GTG
PshAI 2622 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1743 ACCTGGT A^CC[AT]GGT
SphI 2694 GCATGC GCATG^C
SspBI 1726 TGTACA T^GTACA
StuI 2321 AGGCCT AGG^CCT
Tth111I 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1738 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 459 GACGTC GACGT^C
1063 GACGTC
Acc65I 189 GGTACC G^GTACC
2796 GGTACC
Alw44I 114 GTGCAC G^TGCAC
1326 GTGCAC
AlwNI 2715 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2938 CAGATGCTG
ApaLI 114 GTGCAC G^TGCAC
1326 GTGCAC
Asp718I 189 GGTACC G^GTACC
2796 GGTACC
AviII 742 TGCGCA TGC^GCA
1033 TGCGCA
BamHI 1172 GGATCC G^GATCC
1471 GGATCC
BbeI 158 GGCGCC GGCGC^C
2615 GGCGCC
BbsI 297 GTCTTC GAAGAC(2/6)
495 GTCTTC
BbvCI 1916 CCTCAGC CCTCAGC(-5/-2)
768 GCTGAGG
BciVI 1267 GGATAC GTATCC(6/5)
2288 GTATCC
BpuAI 297 GTCTTC GAAGAC(2/6)
495 GTCTTC
BseRI 1914 CTCCTC GAGGAG(10/8)
1147 GAGGAG
BsmBI 1065 CGTCTC CGTCTC(1/5)
1279 CGTCTC
FspI 742 TGCGCA TGC^GCA
1033 TGCGCA
KasI 158 GGCGCC G^GCGCC
2615 GGCGCC
KpnI 189 GGTACC GGTAC^C
2796 GGTACC
KspI 182 CCGCGG CCGC^GG
586 CCGCGG
NarI 158 GGCGCC GG^CGCC
2615 GGCGCC
SacII 182 CCGCGG CCGC^GG
586 CCGCGG
SfoI 158 GGCGCC GGC^GCC
2615 GGCGCC
TaqI 323 TCGA T^CGA
1167 TCGA
Tsp509I 2863 AATT ^AATT
3006 AATT
TthHB8I 323 TCGA T^CGA
1167 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AocI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssHII BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TfiI Tru9I TseI
Tsp45I TspRI XhoII XmaCI XmaI