################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AccIII AclI AcsI AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AvrII BcgI BclI BlnI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eam1105I Eco47III EcoNI EcoRI EcoT22I FbaI FseI HincII HindII HindIII HpaI MfeI MluI MroI MunI NheI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflII 2090 CTTAAG C^TTAAG AspI 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 895 TGGCCA TGG^CCA BbrPI 117 CACGTG CAC^GTG BfrI 2090 CTTAAG C^TTAAG BglI 888 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1204 AGATCT A^GATCT BlpI 2720 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2720 GCTGAGC GC^T[ACGT]AGC BsaAI 117 CACGTG [CT]AC^GT[GA] BsaBI 1268 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 135 TCCGGT [AT]^CCGG[AT] BsiEI 226 CGGCCG CG[GA][CT]^CG Bsp1407I 1726 TGTACA T^GTACA BsrBI 2390 CCGCTC CCGCTC(-3/-3) BsrFI 1140 GCCGGC [GA]^CCGG[CT] BsrGI 1726 TGTACA T^GTACA BstAPI 2153 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2720 GCTGAGC GC^T[ACGT]AGC Cfr10I 1140 GCCGGC [GA]^CCGG[CT] DraI 2757 TTTAAA TTT^AAA DraIII 1742 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 226 CGGCCG C^GGCCG EclXI 226 CGGCCG C^GGCCG Eco52I 226 CGGCCG C^GGCCG EcoRV 754 GATATC GAT^ATC MamI 1268 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 895 TGGCCA TGG^CCA MscI 895 TGGCCA TGG^CCA NaeI 1140 GCCGGC GCC^GGC NdeI 1307 CATATG CA^TATG NgoMIV 1140 GCCGGC G^CCGGC NotI 225 GCGGCCGC GC^GGCCGC NspI 2694 GCATGC [GA]CATG^[CT] PflFI 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1738 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 117 CACGTG CAC^GTG PmlI 117 CACGTG CAC^GTG PshAI 2622 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1743 ACCTGGT A^CC[AT]GGT SphI 2694 GCATGC GCATG^C SspBI 1726 TGTACA T^GTACA StuI 2321 AGGCCT AGG^CCT Tth111I 243 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1738 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AatII 459 GACGTC GACGT^C 1063 GACGTC Acc65I 189 GGTACC G^GTACC 2796 GGTACC Alw44I 114 GTGCAC G^TGCAC 1326 GTGCAC AlwNI 2715 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2938 CAGATGCTG ApaLI 114 GTGCAC G^TGCAC 1326 GTGCAC Asp718I 189 GGTACC G^GTACC 2796 GGTACC AviII 742 TGCGCA TGC^GCA 1033 TGCGCA BamHI 1172 GGATCC G^GATCC 1471 GGATCC BbeI 158 GGCGCC GGCGC^C 2615 GGCGCC BbsI 297 GTCTTC GAAGAC(2/6) 495 GTCTTC BbvCI 1916 CCTCAGC CCTCAGC(-5/-2) 768 GCTGAGG BciVI 1267 GGATAC GTATCC(6/5) 2288 GTATCC BpuAI 297 GTCTTC GAAGAC(2/6) 495 GTCTTC BseRI 1914 CTCCTC GAGGAG(10/8) 1147 GAGGAG BsmBI 1065 CGTCTC CGTCTC(1/5) 1279 CGTCTC FspI 742 TGCGCA TGC^GCA 1033 TGCGCA KasI 158 GGCGCC G^GCGCC 2615 GGCGCC KpnI 189 GGTACC GGTAC^C 2796 GGTACC KspI 182 CCGCGG CCGC^GG 586 CCGCGG NarI 158 GGCGCC GG^CGCC 2615 GGCGCC SacII 182 CCGCGG CCGC^GG 586 CCGCGG SfoI 158 GGCGCC GGC^GCC 2615 GGCGCC TaqI 323 TCGA T^CGA 1167 TCGA Tsp509I 2863 AATT ^AATT 3006 AATT TthHB8I 323 TCGA T^CGA 1167 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AocI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssHII BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI XhoII XmaCI XmaI