##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbvCI BcgI BglI BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DrdI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
KasI KpnI MluI MroI NaeI
NarI NcoI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PshAI PshBI PvuI RsrII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2906 GACGTC GACGT^C
AclI 1064 AACGTT AA^CGTT
AflII 334 CTTAAG C^TTAAG
AflIII 1929 ACGTGT A^C[GA][CT]GT
AocI 1958 CCTCAGG CC^T[ACGT]AGG
Asp700I 2302 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1954 GGATCC G^GATCC
BbrPI 1928 CACGTG CAC^GTG
BciVI 1454 GTATCC GTATCC(6/5)
BfrI 334 CTTAAG C^TTAAG
BsaI 159 GAGACC GGTCTC(1/5)
BsmBI 1831 CGTCTC CGTCTC(1/5)
BsmI 1871 GCATTC GAATGC(1/-1)
Bsp1407I 457 TGTACA T^GTACA
BspHI 1906 TCATGA T^CATGA
BsrDI 1626 GCAATG GCAATG(2/0)
BsrGI 457 TGTACA T^GTACA
Bsu36I 1958 CCTCAGG CC^T[ACGT]AGG
CfoI 1414 GCGC GCG^C
Eco81I 1958 CCTCAGG CC^T[ACGT]AGG
EcoRI 1707 GAATTC G^AATTC
HgaI 2081 GACGC GACGC(5/10)
HhaI 1414 GCGC GCG^C
HinP1I 1414 GCGC G^CGC
HincII 2860 GTTAAC GT[CT]^[GA]AC
HindII 2860 GTTAAC GT[CT]^[GA]AC
HindIII 1519 AAGCTT A^AGCTT
HpaI 2860 GTTAAC GTT^AAC
KspI 1444 CCGCGG CCGC^GG
MfeI 2604 CAATTG C^AATTG
MunI 2604 CAATTG C^AATTG
NdeI 945 CATATG CA^TATG
NheI 2325 GCTAGC G^CTAGC
PmaCI 1928 CACGTG CAC^GTG
PmeI 925 GTTTAAAC GTTT^AAAC
PmlI 1928 CACGTG CAC^GTG
PpuMI 1362 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1064 AACGTT AA^CGTT
PvuII 496 CAGCTG CAG^CTG
RcaI 1906 TCATGA T^CATGA
SacII 1444 CCGCGG CCGC^GG
SapI 690 GAAGAGC GCTCTTC(1/4)
SexAI 1737 ACCAGGT A^CC[AT]GGT
SspBI 457 TGTACA T^GTACA
SspI 187 AATATT AAT^ATT
StuI 1962 AGGCCT AGG^CCT
XbaI 2916 TCTAGA T^CTAGA
XmnI 2302 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccI 680 GTAGAC GT^[AC][GT]AC
2138 GTCTAC
AccII 1413 CGCG CG^CG
1445 CGCG
AcyI 2081 GACGCC G[GA]^CG[CT]C
2906 GACGTC
Alw44I 748 GTGCAC G^TGCAC
1819 GTGCAC
AlwNI 1361 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1781 CAGCTACTG
ApaLI 748 GTGCAC G^TGCAC
1819 GTGCAC
BalI 441 TGGCCA TGG^CCA
1038 TGGCCA
BanI 1572 GGCACC G^G[CT][GA]CC
1640 GGCACC
BclI 632 TGATCA T^GATCA
2269 TGATCA
BglII 649 AGATCT A^GATCT
2825 AGATCT
BpmI 65 CTCCAG CTGGAG(16/14)
1778 CTCCAG
BsaAI 1811 TACGTG [CT]AC^GT[GA]
1928 CACGTG
BsaBI 859 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1898 GATAAAGATC
BsaHI 2081 GACGCC G[GA]^CG[CT]C
2906 GACGTC
BseRI 1337 CTCCTC GAGGAG(10/8)
2360 GAGGAG
BsiEI 1538 CGGCCG CG[GA][CT]^CG
1948 CGGCCG
BsmAI 159 GAGAC GTCTC(1/5)
1832 GTCTC
BspMI 162 ACCTGC ACCTGC(4/8)
1474 GCAGGT
BsrFI 74 GCCGGT [GA]^CCGG[CT]
1281 GCCGGT
BstAPI 646 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1850 GCATATTATGC
BstBI 1503 TTCGAA TT^CGAA
2629 TTCGAA
BstUI 1413 CGCG CG^CG
1445 CGCG
Cfr10I 74 GCCGGT [GA]^CCGG[CT]
1281 GCCGGT
DraIII 1042 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1044 CACAGTGTG
EagI 1538 CGGCCG C^GGCCG
1948 CGGCCG
EclXI 1538 CGGCCG C^GGCCG
1948 CGGCCG
Eco52I 1538 CGGCCG C^GGCCG
1948 CGGCCG
EcoT14I 846 CCAAGG C^C[AT][AT]GG
1965 CCTTGG
FbaI 632 TGATCA T^GATCA
2269 TGATCA
Hin1I 2081 GACGCC G[GA]^CG[CT]C
2906 GACGTC
MamI 859 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1898 GATAAAGATC
MluNI 441 TGGCCA TGG^CCA
1038 TGGCCA
MscI 441 TGGCCA TGG^CCA
1038 TGGCCA
MspA1I 496 CAGCTG C[AC]G^C[GT]G
1444 CCGCGG
MvnI 1413 CGCG CG^CG
1445 CGCG
NspI 1511 GCATGT [GA]CATG^[CT]
2792 ACATGC
NspV 1503 TTCGAA TT^CGAA
2629 TTCGAA
PflMI 108 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
444 CCAAGCTATGG
SacI 766 GAGCTC GAGCT^C
1334 GAGCTC
SfuI 1503 TTCGAA TT^CGAA
2629 TTCGAA
StyI 846 CCAAGG C^C[AT][AT]GG
1965 CCTTGG
Van91I 108 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
444 CCAAGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII