##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BciVI BlnI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FseI FspI
HincII HindII HpaI HphI KpnI
KspI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1319 GTCTAC GT^[AC][GT]AC
AccII 2456 CGCG CG^CG
AcyI 631 GGCGCC G[GA]^CG[CT]C
AflII 459 CTTAAG C^TTAAG
AflIII 2803 ACATGT A^C[GA][CT]GT
AlwNI 1000 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1440 ATTAAT AT^TAAT
AsnI 1440 ATTAAT AT^TAAT
Asp700I 1563 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 556 CTCGAG C^[CT]CG[GA]G
BamHI 254 GGATCC G^GATCC
BanI 631 GGCGCC G^G[CT][GA]CC
BbeI 631 GGCGCC GGCGC^C
BbsI 2367 GAAGAC GAAGAC(2/6)
BfrI 459 CTTAAG C^TTAAG
BlpI 1674 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1674 GCTGAGC GC^T[ACGT]AGC
BpuAI 2367 GAAGAC GAAGAC(2/6)
BsaAI 1482 TACGTG [CT]AC^GT[GA]
BsaHI 631 GGCGCC G[GA]^CG[CT]C
BsaI 489 GAGACC GGTCTC(1/5)
BsoBI 556 CTCGAG C^[CT]CG[GA]G
BspHI 2284 TCATGA T^CATGA
BspLU11I 2803 ACATGT A^CATGT
BstAPI 1289 GCAAGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2259 GGTTACC G^GT[ACGT]ACC
BstPI 2259 GGTTACC G^GT[ACGT]ACC
BstUI 2456 CGCG CG^CG
CelII 1674 GCTGAGC GC^T[ACGT]AGC
DraII 790 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 790 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2259 GGTTACC G^GT[ACGT]ACC
EcoT14I 2036 CCATGG C^C[AT][AT]GG
EcoT22I 1701 ATGCAT ATGCA^T
FauI 1542 CCCGC CCCGC(4/6)
HgaI 15 GCGTC GACGC(5/10)
Hin1I 631 GGCGCC G[GA]^CG[CT]C
KasI 631 GGCGCC G^GCGCC
MslI 1254 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2456 CGCG CG^CG
NarI 631 GGCGCC GG^CGCC
NcoI 2036 CCATGG C^CATGG
NheI 2139 GCTAGC G^CTAGC
NsiI 1701 ATGCAT ATGCA^T
NspI 2803 ACATGT [GA]CATG^[CT]
PaeR7I 556 CTCGAG C^TCGAG
PshBI 1440 ATTAAT AT^TAAT
PvuII 670 CAGCTG CAG^CTG
RcaI 2284 TCATGA T^CATGA
SexAI 2065 ACCAGGT A^CC[AT]GGT
SfoI 631 GGCGCC GGC^GCC
StyI 2036 CCATGG C^C[AT][AT]GG
XhoI 556 CTCGAG C^TCGAG
XmnI 1563 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 142 AAATTC [GA]^AATT[CT]
2725 AAATTT
ApoI 142 AAATTC [GA]^AATT[CT]
2725 AAATTT
AspHI 1923 GAGCAC G[AT]GC[AT]^C
1941 GAGCAC
BalI 313 TGGCCA TGG^CCA
568 TGGCCA
BanII 559 GAGCCC G[GA]GC[CT]^C
2280 GGGCTC
BclI 106 TGATCA T^GATCA
1813 TGATCA
BglI 497 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
561 GCCCGTCTGGC
BglII 846 AGATCT A^GATCT
1973 AGATCT
BsaBI 101 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2627 GATACTGATC
BsgI 1296 GTGCAG GTGCAG(16/14)
2412 GTGCAG
BsiHKAI 1923 GAGCAC G[AT]GC[AT]^C
1941 GAGCAC
BsmI 1699 GAATGC GAATGC(1/-1)
2079 GAATGC
BsrDI 199 CATTGC GCAATG(2/0)
344 CATTGC
EaeI 313 TGGCCA [CT]^GGCC[GA]
568 TGGCCA
FbaI 106 TGATCA T^GATCA
1813 TGATCA
HaeII 30 AGCGCC [GA]GCGC^[CT]
631 GGCGCC
HindIII 456 AAGCTT A^AGCTT
2056 AAGCTT
MaeII 1483 ACGT A^CGT
1604 ACGT
MamI 101 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2627 GATACTGATC
MluNI 313 TGGCCA TGG^CCA
568 TGGCCA
MscI 313 TGGCCA TGG^CCA
568 TGGCCA
PflMI 374 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGAGGATGG
StuI 1535 AGGCCT AGG^CCT
2739 AGGCCT
Van91I 374 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1446 CCAGAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII