##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccIII AclI AflII AflIII AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I AspEI AvrII BbeI
BbrPI BcgI BfrI BglII BlnI
BsaAI BseAI BsgI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CpoI DraI DrdI Eam1105I Eco81I
EcoRV EcoT22I FseI HindIII HpaI
KasI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SapI SbfI
ScaI SexAI SfoI SfuI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 598 GACGTC GACGT^C
Acc65I 501 GGTACC G^GTACC
AccI 1317 GTCGAC GT^[AC][GT]AC
Aor51HI 2013 AGCGCT AGC^GCT
Asp718I 501 GGTACC G^GTACC
AspI 595 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 483 CCCGGG C^[CT]CG[GA]G
BbsI 1256 GAAGAC GAAGAC(2/6)
BbvCI 13 GCTGAGG CCTCAGC(-5/-2)
BciVI 2302 GGATAC GTATCC(6/5)
BglI 61 GCCTGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1256 GAAGAC GAAGAC(2/6)
BsmBI 937 GAGACG CGTCTC(1/5)
BsoBI 483 CCCGGG C^[CT]CG[GA]G
BspDI 793 ATCGAT AT^CGAT
BspHI 875 TCATGA T^CATGA
BsrDI 144 CATTGC GCAATG(2/0)
BssHII 32 GCGCGC G^CGCGC
BstEII 1900 GGTGACC G^GT[ACGT]ACC
BstPI 1900 GGTGACC G^GT[ACGT]ACC
BstXI 50 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 793 ATCGAT AT^CGAT
DraIII 446 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 466 CGGCCG C^GGCCG
EarI 1543 CTCTTC CTCTTC(1/4)
EclXI 466 CGGCCG C^GGCCG
Eco47III 2013 AGCGCT AGC^GCT
Eco52I 466 CGGCCG C^GGCCG
EcoNI 1905 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1900 GGTGACC G^GT[ACGT]ACC
KpnI 501 GGTACC GGTAC^C
Ksp632I 1543 CTCTTC CTCTTC(1/4)
MaeII 599 ACGT A^CGT
NaeI 222 GCCGGC GCC^GGC
NgoMIV 222 GCCGGC G^CCGGC
PflFI 595 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 875 TCATGA T^CATGA
SacI 2226 GAGCTC GAGCT^C
SalI 1317 GTCGAC G^TCGAC
SfiI 60 GGCCTGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 483 CCCGGG CCC^GGG
SphI 1178 GCATGC GCATG^C
Tth111I 595 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AgeI 23 ACCGGT A^CCGGT
758 ACCGGT
AlwNI 1039 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1590 CAGCTGCTG
ApaI 251 GGGCCC GGGCC^C
487 GGGCCC
BamHI 1012 GGATCC G^GATCC
1212 GGATCC
BclI 1054 TGATCA T^GATCA
2151 TGATCA
BsaBI 1428 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2249 GATGGACATC
BsaWI 23 ACCGGT [AT]^CCGG[AT]
758 ACCGGT
BsiEI 466 CGGCCG CG[GA][CT]^CG
493 CGACCG
BstAPI 1718 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2370 GCACCGCCTGC
FbaI 1054 TGATCA T^GATCA
2151 TGATCA
HaeII 900 GGCGCT [GA]GCGC^[CT]
2013 AGCGCT
HincII 151 GTTGAC GT[CT]^[GA]AC
1317 GTCGAC
HindII 151 GTTGAC GT[CT]^[GA]AC
1317 GTCGAC
KspI 418 CCGCGG CCGC^GG
496 CCGCGG
MamI 1428 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2249 GATGGACATC
NspI 1178 GCATGC [GA]CATG^[CT]
1995 ACATGC
PflMI 369 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGTGGCTGG
PinAI 23 ACCGGT A^CCGGT
758 ACCGGT
SacII 418 CCGCGG CCGC^GG
496 CCGCGG
SgrAI 22 CACCGGTG C[GA]^CCGG[CT]G
282 CGCCGGTG
TfiI 651 GAATC G^A[AT]TC
1375 GAATC
Van91I 369 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
AviII BalI BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII